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Vol. 30 No. 8 (2018): Vol 30 Issue 8

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Stopped-Flow Injection and Spectrophotometric Methods of Quercetin Dihydrate Determination by Using Two Reagents (2,4-Dichloroaniline and p-Aminoacetophenone)

https://doi.org/10.14233/ajchem.2018.21185
Hinda Ali Mahmood
Department of Chemistry, College of Science, University of Baghdad, Jadriyah, Baghdad, Iraq *Corresponding author: E-mail: sadeem.subhi@yahoo.com
Sadeem Subhi Abed*
Department of Chemistry, College of Science, University of Baghdad, Jadriyah, Baghdad, Iraq *Corresponding author: E-mail: sadeem.subhi@yahoo.com

Asian Journal of Chemistry, Vol. 30 No. 8 (2018): Vol 30 Issue 8

Abstract

A stopped flow injection and spectrophotometric method were used to estimate of quercetin dihydrate in pure and supplement preparations were suggested. This method was depend on coupling reaction between quercetin dihydrate and diazotized reagents 2,4-dichloroaniline (method A) and p-aminoacetophenone (method B) in alkaline medium to form water soluble with orange colour dyes. These dyes are steady and have a maximum absorbance at 420 nm in method A and 413 nm in method B. Calibration graphs showed that a Beer's law is obeyed over the range of concentration 0.4-50 and 0.4-45 μg mL-1 for quercetein dihydrate with a detection limit 0.3715 and 0.385 for method A and B, respectively. The sample through put is 27 and 24 for method A and B, respectively. The suggested methods were successfully applied for the evaluation of quercetin dihydrate in dietary supplements.

Keywords

Flavonoids Quercetin dihydrate Stopped flow injection Spectrophotometric methods.
Ali Mahmood, H., & Subhi Abed*, S. (2018). Stopped-Flow Injection and Spectrophotometric Methods of Quercetin Dihydrate Determination by Using Two Reagents (2,4-Dichloroaniline and p-Aminoacetophenone). Asian Journal of Chemistry, 30(8), 1691–1695. https://doi.org/10.14233/ajchem.2018.21185
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