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Vol. 26 No. 8 (2014): Vol 26 Issue 8

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Simultaneous Determination of Three Active Ingredients in Schisandra chinensis (Turcz.) Baill by HPLC

https://doi.org/10.14233/ajchem.2014.16342
Weiming Cheng
Zhejiang Institute for Food and Drug Control, No 86 Lane 1 Jichang Road Jianggan Dist. 310004, Hangzhou Zhejiang Province, P.R. China
Jie Gao
Grade 2011, Zhejiang University of TCM, No. 548, Binwen Road, Binjiang Dist. 310053, Hangzhou Zhejiang Province, P.R. China
Shan Zhong
State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University (Guangzhou Medical University), No. 151 YanJiang Road Yuexiu Dist. 510120, Guangzhou, Guangdong Province, P.R. China

Corresponding Author(s) : Shan Zhong

zhong-shan2000@hotmail.com

Asian Journal of Chemistry, Vol. 26 No. 8 (2014): Vol 26 Issue 8

Abstract

In present study, an HPLC method was setup for the simultaneous determination of 3 active ingredients in Schisandra chinensis (Turcz.) Baill. HPLC conditions included Kromasil-C18 column (250 × 4.6 mm, 5 μm) and the mobile phase was a mixture of MeOH-CH3CN-H2O (5:1:4). The flow rate was 1 mL/min, the column temperature was at 30 °C and the UV detection wavelength was 246 nm. The 3 active ingredients had good linear relationships i.e., schizandrin in the range of 0.10-6.0 μg (R2 = 0.999 8), deoxyschizandrin in the range of 0.15-9.0 μg (R2 = 0.999 2) and schizandrin B in the range of 0.05-3.0 μg (R2 = 0.999 7). Their average recoveries were 99.3 % (RSD = 1.3 %), 97.1 % (RSD = 2 %) and 95.2 % (RSD = 2 %), respectively. This method was steady with high precision and good repeatability and could be used for the determination of the 3 active ingredients and quality control of S. chinensis.

Keywords

Schisandra chinensis Schizandrin Deoxyschizandrin Schizandrin B HPLC
Cheng, W., Gao, J., & Zhong, S. (2014). Simultaneous Determination of Three Active Ingredients in Schisandra chinensis (Turcz.) Baill by HPLC. Asian Journal of Chemistry, 26(8), 2434–2436. https://doi.org/10.14233/ajchem.2014.16342
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References
  1. W. Lin, Z.X. Huang, G.M. Chen, C.F. Lin and X.C. Zhong, Strait J. Preventive Medicine, 4, 51 (2009).
  2. X.G. Li, Q. Gao, W. Wen, P.F. Zhang, F. Xiao and H.M. Luo, J. Chin. Med.Mater., 28, 156 (2005).
  3. C.L. Li, S. Zhang and Z.Q. Liu, J. Zhangjiakou Med. College, 12, 123 (2002).
  4. L.H. Yu and G.T. Liu, Progr. Chem., 21, 66 (2009).
  5. J. Zaugg, S.N. Ebrahimi, M. Smiesko, I. Baburin, S. Hering and M. Hamburger, Phytochemistry, 72, 2385 (2011); doi:10.1016/j.phytochem.2011.08.014.
  6. T. Lu, H. Chen, Z. Huang, B. Cai, J. Hu, C. Mao and X. Gong, Pharmacogn. Maganize, 9, 155 (2013); doi:10.4103/0973-1296.111280.
  7. X. Ji, Y. Naito, G. Hirokawa, H. Weng, Y. Hiura, R. Takahashi and N. Iwai, Hypertens. Res., 35, 173 (2012); doi:10.1038/hr.2011.153.
  8. C.H. Ma, L. Yang, F.J. Yang, W.J. Wang, C.J. Zhao and Y.G. Zu, Int. J. Mol. Sci., 13, 14294 (2012); doi:10.3390/ijms131114294.
  9. J. Li, H.- Zhao, X.- Ruan, Y.- Xu, W.- Meng, K.- Li and J.- Zhang, J. Zhejiang Univ. Sci. B, 6, 1210 (2005); doi:10.1631/jzus.2005.B1210.
  10. E.H. Choi, N. Lee, H.J. Kim, M.K. Kim, S.-G. Chi, D.Y. Kwon and H.S. Chun, Genes. Nutr., 2, 337 (2008); doi:10.1007/s12263-007-0073-y.
  11. H.J. Yang and S.M. Yang, J. Chinese Med. Mater., 26, 618 (2004).
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