Analysis of Isoliquiritin and Isoliquiritigenin from Insampaedoksan Fermented by Lactobacillus plantarum 144 Using RP-HPLC

K.J. Lee; P.M. Jung; J.H. Roh; J.Y. Ma

doi:10.14233/ajchem.2014.16154

Issue

Vol. 26 No. 8 (2014): Vol 26 Issue 8

Issue Published : April 15, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Analysis of Isoliquiritin and Isoliquiritigenin from Insampaedoksan Fermented by Lactobacillus plantarum 144 Using RP-HPLC

https://doi.org/10.14233/ajchem.2014.16154

K.J. Lee

Korean Institute of Oriental Medicine, KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea

P.M. Jung

Korean Institute of Oriental Medicine, KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea

J.H. Roh

Korean Institute of Oriental Medicine, KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea

J.Y. Ma

Korean Institute of Oriental Medicine, KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea

Corresponding Author(s) : J.Y. Ma

jyma@kiom.re.kr

Asian Journal of Chemistry, Vol. 26 No. 8 (2014): Vol 26 Issue 8
Article Published : April 16, 2014

Abstract

Insampaedoksan is a traditional herbal medicine used for antipyretic and antiinflammatory diseases. Thus isoliquiritigenin have bioactivity effects such as antioxidant, estrogenic and anticancer. The level of isoliquiritin and isoliquiritigenin in insampaedoksan were investigated before and after fermentation with Lactobacillus plantarum 144, along with isoliquiritin and isoliquiritigenin extraction efficiency with 100 % water, 60 % aqueous MeOH and 100 % MeOH. The characterization of isoliquiritin and isoliquiritigenin were achieved by comparing its retention time (t_R), molecular weight and UV absorption with those of the standard compound. The extraction efficiency of isoliquiritin and isoliquiritigenin were the highest with 100 % MeOH. After fermentation, the amount of 100 % MeOH extract isoliquiritigenin was selectively increased (202.73 %) and isoliquiritin was selectively decreased (82.57 %). The bioconversion rate of isoliquiritin to isoliquritigenin was 39.93 %. Also, the molecular mass of the fermented insampaedoksan fraction (#1) were shown via LC-MS and, its high purity was shown to be above > 95 %. Thus, qualitative and quantitative analyses of isoliquiritin and isoliquiritigenin in insampaedoksan and fermented insampaedoksan were conducted.

Keywords

Analysis Bioconversion HPLC-DAD Insampaedoksan Isolation

Lee, K., Jung, P., Roh, J., & Ma, J. (2014). Analysis of Isoliquiritin and Isoliquiritigenin from Insampaedoksan Fermented by Lactobacillus plantarum 144 Using RP-HPLC. Asian Journal of Chemistry, 26(8), 2425–2428. https://doi.org/10.14233/ajchem.2014.16154

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References

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J. Tilburt, Bull. World Health Organ., 86, 594 (2008); doi:10.2471/BLT.07.042820.

J.H. Kim, W.J. Lee, Y.W. Cho and K.Y. Kim, J. Kor. Soc. Food Sci. Nutr, 38, 787 (2009); doi:10.3746/jkfn.2009.38.6.787.

E.S. Doh, J.P. Chang, K.H. Lee and N.S. Seong, Kor. J. Med. Crop Sci, 18, 255 (2010).

J.S. Hyon, S.M. Kang, S.W. Han, M.C. Kang, M.C. Oh, C.K. Oh, D.-W. Kim, Y.-J. Jeon and S.-H. Kim, J. Kor. Soc. Food Sci. Nutr, 38, 1310 (2009); doi:10.3746/jkfn.2009.38.10.1310.

D. Nakano, C.J. Kwak, K. Fuji, K. Ikemura, A. Satake, M. Ohkita, M. Takaoka, Y. Ohno, M. Nakai, N. Tomomori, Y. Kiso and Y. Matsumura, J. Pharmacol. Exp. Ther., 318, 328 (2006); doi:10.1124/jpet.105.100149.

Y.W. Lin and B.H. Chiang, Process Biochem., 43, 244 (2008); doi:10.1016/j.procbio.2007.11.020.

S. Bose, M.Y. Song, J.K. Nam, M.J. Lee and H.J. Kim, Food Chem., 134, 758 (2012); doi:10.1016/j.foodchem.2012.02.175.

H.A. Eum, J.H. Lee, M.C. Yang, K.S. Shim, J.H. Lee and J.Y. Ma, J. Tradit. Complement. Altern. Med, 8, 312 (2011).

Y.C. Oh, W.K. Cho, J.H. Oh, G.Y. Im, Y.H. Jeong, M.C. Yang and J.Y. Ma, BMC Complement. Altern. Med., 12, 17 (2012); doi:10.1186/1472-6882-12-17.

X. Chen, J. Fruehauf, J.D. Goldsmith, H. Xu, K.K. Katchar, H.W. Koon, D. Zhao, E.G. Kokkotou, C. Pothoulakis and C.P. Kelly, Gastroenterol., 137, 914 (2009); doi:10.1053/j.gastro.2009.05.050.

H. Haraguchi, H. Ishikawa, K. Mizutani, Y. Tamura and T. Kinoshita, Med. Chem., 6, 339 (1998).

C. Hu, H. Liu, J. Du, B. Mo, H. Qi, X. Wang, S. Ye and Z. Li, J. Steroid Biochem. Mol. Biol., 113, 209 (2009); doi:10.1016/j.jsbmb.2008.12.019.

M. Baba, R. Asano, I. Takigami, T. Takahashi, M. Ohmura, Y. Okada, H. Sugimoto, T. Arika, H. Nishino and T. Okuyama, Biol. Pharm. Bull., 25, 247 (2002); doi:10.1248/bpb.25.247.

M. Kanazawa, Y. Satomi, Y. Mizutani, O. Ukimura, A. Kawauchi, T. Sakai, M. Baba, T. Okuyama, H. Nishino and T. Miki, Eur. Urol., 43, 580 (2003); doi:10.1016/S0302-2838(03)00090-3.

K.J. Lee, M.J. Gu, J.H. Roh, P.M. Jung and J.Y. Ma, Yakkak Hoeji, 57, 1 (2013).

J. Parekh, D. Jadeja and S. Chanda, Turk. J. Biol., 29, 203 (2005).

J.M. Halket, D. Waterman, A.M. Przyborowska, R.K.P. Pakel, P.D. Fraser and P.M. Bramley, J. Exp. Bot., 56, 219 (2004); doi:10.1093/jxb/eri069.

J.A. Marazza, M.S. Garro and G. Savoy de Giori, Food Microbiol., 26, 333 (2009); doi:10.1016/j.fm.2008.11.004.

S.P. Ham, B.L. Lim, J.H. Ka, S.O. Ka, B.H. Park, Korean J. Orient. Physiol. Pathol., 22, 340 (2008).

Issue

Vol. 26 No. 8 (2014): Vol 26 Issue 8

Analysis of Isoliquiritin and Isoliquiritigenin from Insampaedoksan Fermented by Lactobacillus plantarum 144 Using RP-HPLC

Corresponding Author(s) : J.Y. Ma

Abstract

Keywords

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