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Vol. 26 No. 8 (2014): Vol 26 Issue 8

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Extraction of Chlorophyll-a from Eutrophic Water by Repeated Freezing and Thawing-Extraction Method

https://doi.org/10.14233/ajchem.2014.15700
G.M. Zeng
Faculty of Urban Construction and Environmental Engineering, Key Laboratory of Three Gorges Reservoir Region's Eco-Environment (Ministry of Education), Chongqing University, Sha Zheng Street, Chongqing 400045, P.R. China
J. Zhou
Faculty of Urban Construction and Environmental Engineering, Key Laboratory of Three Gorges Reservoir Region's Eco-Environment (Ministry of Education), Chongqing University, Sha Zheng Street, Chongqing 400045, P.R. China
T. Huang
Faculty of Urban Construction and Environmental Engineering, Key Laboratory of Three Gorges Reservoir Region's Eco-Environment (Ministry of Education), Chongqing University, Sha Zheng Street, Chongqing 400045, P.R. China
S.Y. Liu
Faculty of Urban Construction and Environmental Engineering, Key Laboratory of Three Gorges Reservoir Region's Eco-Environment (Ministry of Education), Chongqing University, Sha Zheng Street, Chongqing 400045, P.R. China
F.F. Ji
Faculty of Urban Construction and Environmental Engineering, Key Laboratory of Three Gorges Reservoir Region's Eco-Environment (Ministry of Education), Chongqing University, Sha Zheng Street, Chongqing 400045, P.R. China
P. Wang
Faculty of Urban Construction and Environmental Engineering, Key Laboratory of Three Gorges Reservoir Region's Eco-Environment (Ministry of Education), Chongqing University, Sha Zheng Street, Chongqing 400045, P.R. China

Corresponding Author(s) : J. Zhou

zhoujiantt@126.com

Asian Journal of Chemistry, Vol. 26 No. 8 (2014): Vol 26 Issue 8

Abstract

This work described the extraction processes of chlorophylls was analyze during two extraction techniques, namely the cool acetone method and acetone repeated freezing and thawing-extraction method as the chlorophyll-a extraction agent, and the results were compared. The objective of this study was to use the uniform design to optimization of the extraction of chlorophyll-a from freshwater green algae by two different methods used acetone. Extracted chlorophyll-a was investigated by fourier transform infrared spectroscopy. These results suggested that there is significant statistical difference between these two methods. The acetone repeated freezing and thawing-extraction method has more advantages than the rubbing-acetone method, which is faster and easier to handle, more complete extraction and lower toxic.

Keywords

Chlorophyll a Rubbing Uniform design Repeated freezing and thawing-extraction
Zeng, G., Zhou, J., Huang, T., Liu, S., Ji, F., & Wang, P. (2014). Extraction of Chlorophyll-a from Eutrophic Water by Repeated Freezing and Thawing-Extraction Method. Asian Journal of Chemistry, 26(8), 2289–2292. https://doi.org/10.14233/ajchem.2014.15700
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