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Vol. 26 No. 4 (2014): Vol 26 Issue 4

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Study on Alkaline Protease Immobilized on Mesoporous Materials

https://doi.org/10.14233/ajchem.2014.16033
Hong Zhuang
College of Quartermaster Technology, Jilin University, Xi'an Road 5333#, Changchun 130062, P.R. China
Shuting Dong
College of Quartermaster Technology, Jilin University, Xi'an Road 5333#, Changchun 130062, P.R. China
Ting Zhang
College of Quartermaster Technology, Jilin University, Xi'an Road 5333#, Changchun 130062, P.R. China
Ning Tang
College of Quartermaster Technology, Jilin University, Xi'an Road 5333#, Changchun 130062, P.R. China
Na Xu
College of Quartermaster Technology, Jilin University, Xi'an Road 5333#, Changchun 130062, P.R. China
Bin Sun
College of Quartermaster Technology, Jilin University, Xi'an Road 5333#, Changchun 130062, P.R. China
Jingbo Liu
College of Quartermaster Technology, Jilin University, Xi'an Road 5333#, Changchun 130062, P.R. China
Mingdi Zhang
College of Quartermaster Technology, Jilin University, Xi'an Road 5333#, Changchun 130062, P.R. China
Yuan Yuan
College of Quartermaster Technology, Jilin University, Xi'an Road 5333#, Changchun 130062, P.R. China

Corresponding Author(s) : Yuan Yuan

happymissdong@sina.cn

Asian Journal of Chemistry, Vol. 26 No. 4 (2014): Vol 26 Issue 4

Abstract

In the present study, MCM-41 and SBA-15 were used to immobilize alkaline protease. SBA-15 was selected as the better material to immobilize alkaline protease because of its higher enzyme loading and energy recovery of immobilized alkaline protease. Factors affecting the catalytic activity of the immobilized enzyme, such as immobilization's time, pH and enzyme concentration were studied. The properties of immobilized enzyme were also investigated. Under the optimal condition (1 h, pH 8, enzyme concentration 10 mg/mL), the immobilized enzyme displayed maximum activity (enzyme loading 589.43 μg/mg, energy recovery 70.86 %). Obtained immobilized enzyme had a good pH and temperature stability.

Keywords

Mesoporous materials Immobilization Alkaline protease Stability
Zhuang, H., Dong, S., Zhang, T., Tang, N., Xu, N., Sun, B., … Yuan, Y. (2014). Study on Alkaline Protease Immobilized on Mesoporous Materials. Asian Journal of Chemistry, 26(4), 1139–1144. https://doi.org/10.14233/ajchem.2014.16033
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