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Fluorescence Spectral Study on the Interaction Between Brilliant Blue and Trypsin
Corresponding Author(s) : Benzhi Liu
Asian Journal of Chemistry,
Vol. 26 No. 4 (2014): Vol 26 Issue 4
Abstract
The interaction between brilliant blue and trypsin was studied by using fluorescent technique. The fluorescence quenching data were analyzed according to Stern-Volmer equation and Line weaver-Burk equation. The binding constants, thermodynamic parameters and binding sites were obtained. According to the thermodynamic parameters, the main binding force between brilliant blue and trypsin was ascertained as electrostatic interaction. The binding distance between brilliant blue and trypsin was also given based on the Förster energy transfer theory.
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- G. Otterstatter, Die Farbung von Lebensmitteln, Arzneimitteln, Kosmetika, Behr's Verlag, Hamburg, edn 2 (1995).
- J.J. Berzas Nevado, C. Guiberteau Cabanillas and A.M. Contento Salcedo, Anal. Chim. Acta, 378, 63 (1999); doi:10.1016/S0003-2670(98)00574-1.
- E.A. Allegretto and J.W. Pike, J. Biol. Chem., 260, 10139 (1985).
- J.R. Lakowicz, Principles of Fluorescence Spectroscopy, Springer Science, Business Media, New York (2006).
- G. Zhang, B. Keita, C.T. Craescu, S. Miron, P. de Oliveira and L. Nadjo, Biomacromolecules, 9, 812 (2008); doi:10.1021/bm701120j.
- J.R. Lakowicz, Principles of Fluorescence Spectroscopy, Plenum Press, New York (1983).
- J.R. Lakowicz and G. Weber, Biochemistry, 12, 4161 (1973); doi:10.1021/bi00745a020.
- W.R. Ware, J. Phys. Chem., 66, 455 (1962); doi:10.1021/j100809a020.
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- M.X. Xie, X.-Y. Xu and Y.-D. Wang, Biochim. Biophys. Acta, 1724, 215 (2005); doi:10.1016/j.bbagen.2005.04.009.
- P.D. Ross and S. Subramanian, Biochemistry, 20, 3096 (1981); doi:10.1021/bi00514a017.
- T. Forster and O. Sinaoglu, Modern Quantum Chemistry, Academic Press, New York, vol. 3 (1965).
- D.C. Saha, K. Ray and T.N. Misra, Spectrochim. Acta A, 56, 797 (2000); doi:10.1016/S1386-1425(99)00169-9.
- C. Bertucci and E. Domenici, Curr. Med. Chem., 9, 1463 (2002); doi:10.2174/0929867023369673.
- B. Valeur and J.C. Brochon, New Trends in Fluorescence Spectroscopy, Springer Press, Berlin, edn 6 (1999).
References
G. Otterstatter, Die Farbung von Lebensmitteln, Arzneimitteln, Kosmetika, Behr's Verlag, Hamburg, edn 2 (1995).
J.J. Berzas Nevado, C. Guiberteau Cabanillas and A.M. Contento Salcedo, Anal. Chim. Acta, 378, 63 (1999); doi:10.1016/S0003-2670(98)00574-1.
E.A. Allegretto and J.W. Pike, J. Biol. Chem., 260, 10139 (1985).
J.R. Lakowicz, Principles of Fluorescence Spectroscopy, Springer Science, Business Media, New York (2006).
G. Zhang, B. Keita, C.T. Craescu, S. Miron, P. de Oliveira and L. Nadjo, Biomacromolecules, 9, 812 (2008); doi:10.1021/bm701120j.
J.R. Lakowicz, Principles of Fluorescence Spectroscopy, Plenum Press, New York (1983).
J.R. Lakowicz and G. Weber, Biochemistry, 12, 4161 (1973); doi:10.1021/bi00745a020.
W.R. Ware, J. Phys. Chem., 66, 455 (1962); doi:10.1021/j100809a020.
J. Min, X. Meng-Xia, Z. Dong, L. Yuan, L. Xiao-Yu and C. Xing, J. Mol. Struct., 692, 71 (2004); doi:10.1016/j.molstruc.2004.01.003.
M.X. Xie, X.-Y. Xu and Y.-D. Wang, Biochim. Biophys. Acta, 1724, 215 (2005); doi:10.1016/j.bbagen.2005.04.009.
P.D. Ross and S. Subramanian, Biochemistry, 20, 3096 (1981); doi:10.1021/bi00514a017.
T. Forster and O. Sinaoglu, Modern Quantum Chemistry, Academic Press, New York, vol. 3 (1965).
D.C. Saha, K. Ray and T.N. Misra, Spectrochim. Acta A, 56, 797 (2000); doi:10.1016/S1386-1425(99)00169-9.
C. Bertucci and E. Domenici, Curr. Med. Chem., 9, 1463 (2002); doi:10.2174/0929867023369673.
B. Valeur and J.C. Brochon, New Trends in Fluorescence Spectroscopy, Springer Press, Berlin, edn 6 (1999).