Copyright (c) 2014 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Rapid Fluorescence Polarization Immunoassay for Highly Sensitive Detection of 2,4,6-Trinitrotoluene
Corresponding Author(s) : Wenzong Lu
Asian Journal of Chemistry,
Vol. 26 No. 3 (2014): Vol 26 Issue 3
Abstract
Detection of explosives is of great significance in public security programmer and environmental science. In the present work, a fluorescence polarization immunoassay based on a monoclonal antibody for the detection of 2,4,6-trinitrotoluene was established and optimized. Fluorescein-labeled (tracer) was synthesized and purified by column chromatography. The immunoassay exhibited excellent sensitivity for the detection of 2,4,6-trinitrotoluene with good stability. The detection limit was 5 μg mL-1, within a response time of 4 min. The cross-reactivity of the method to 1,3,5-trinitrohexahydro-1,3,5-triazine, 2,4,6-trinitrophenol, 2,4,6-trinitrobenzoic acid, 2-nitrotoluene and 2,4-dinitrotoluene, were very low (cross-reactivity < 0.01 %). The method developed is characterized by high specificity and reproducibility (coefficients of variance ranged from 2.7-6.5 % for interassay and from 1.6-4.4 % for intra-assay). The relative error value was less 1 % in assay of simulating environmental sample. The fluorescence polarization immunoassay method can be applied to the screening of environmental sample for 2,4,6-trinitrotoluene without complicated operation.
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- A.A. Adams, P.T. Charles, J.R. Deschamps and A.W. Kusterbeck, Anal. Chem., 83, 8411 (2011); doi:10.1021/ac2009788.
- E.P. Agency, Health advisory for TNT, Criteria and Standard Division, Office of Drinking Water, Washington, DC, USA, (1989).
- B. Balan, C. Vijayakumar, M. Tsuji, A. Saeki and S. Seki, J. Phys. Chem. B, 116, 10371 (2012); doi:10.1021/jp304791r.
- F. Rowell, J. Seviour, A.Y. Lim, C.G. Elumbaring-Salazar, J. Loke and J. Ma, Forensic Sci. Int., 221, 84 (2012); doi:10.1016/j.forsciint.2012.04.007.
- M. Najarro, M.E. Davila Morris, M.E. Staymates, R. Fletcher and G. Gillen, Analyst, 137, 2614 (2012); doi:10.1039/c2an16145a.
- M. Tabrizchi and V. Ilbeigi, J. Hazard. Mater., 176, 692 (2010); doi:10.1016/j.jhazmat.2009.11.087.
- H. Wackerbarth, C. Salb, L. Gundrum, M. Niederkruger, K. Christou, V. Beushausen and W. Viöl, Appl. Opt., 49, 4362 (2010); doi:10.1364/AO.49.004362.
- M.R. Leahy-Hoppa, M.J. Fitch and R. Osiander, Anal. Bioanal. Chem., 395, 247 (2009); doi:10.1007/s00216-009-2803-z.
- K. Wells and D.A. Bradley, Appl. Radiat. Isot., 70, 1729 (2012); doi:10.1016/j.apradiso.2012.01.011.
- D.R. Shankaran, K.V. Gobi, T. Sakai, K. Matsumoto, K. Toko and N. Miura, Biosens. Bioelectron., 20, 1750 (2005); doi:10.1016/j.bios.2004.06.044.
- E.S. Bromage, T. Lackie, M.A. Unger, J. Ye and S.L. Kaattari, Biosens. Bioelectron., 22, 2532 (2007); doi:10.1016/j.bios.2006.10.001.
- M. Altstein, A. Bronshtein, B. Glattstein, A. Zeichner, T. Tamiri and J. Almog, Anal. Chem., 73, 2461 (2001); doi:10.1021/ac001376y.
- Y. Mizuta, T. Onodera, P. Singh, K. Matsumoto, N. Miura and K. Toko, Biosens. Bioelectron., 24, 191 (2008); doi:10.1016/j.bios.2008.03.042.
- G.V. Zyryanov, M.A. Palacios and P. Anzenbacher Jr., Org. Lett., 10, 3681 (2008); doi:10.1021/ol801030u.
- S. Girotti, S. Eremin, A. Montoya, M.J. Moreno, P. Caputo, M. D'Elia, L. Ripani, F.S. Romolo and E. Maiolini, Anal. Bioanal. Chem., 396, 687 (2010); doi:10.1007/s00216-009-3264-0.
- L. Feng, H. Li, Y. Qu and C. Lu, Chem. Commun., 48, 4633 (2012);doi:10.1039/C2CC16115J.
- C.M. Maragos, Mycotoxin Res., 22, 96 (2006); doi:10.1007/BF02956771.
- G.P. Anderson and E.R. Goldman, J. Immunol. Methods, 339, 47 (2008); doi:10.1016/j.jim.2008.08.001.
References
A.A. Adams, P.T. Charles, J.R. Deschamps and A.W. Kusterbeck, Anal. Chem., 83, 8411 (2011); doi:10.1021/ac2009788.
E.P. Agency, Health advisory for TNT, Criteria and Standard Division, Office of Drinking Water, Washington, DC, USA, (1989).
B. Balan, C. Vijayakumar, M. Tsuji, A. Saeki and S. Seki, J. Phys. Chem. B, 116, 10371 (2012); doi:10.1021/jp304791r.
F. Rowell, J. Seviour, A.Y. Lim, C.G. Elumbaring-Salazar, J. Loke and J. Ma, Forensic Sci. Int., 221, 84 (2012); doi:10.1016/j.forsciint.2012.04.007.
M. Najarro, M.E. Davila Morris, M.E. Staymates, R. Fletcher and G. Gillen, Analyst, 137, 2614 (2012); doi:10.1039/c2an16145a.
M. Tabrizchi and V. Ilbeigi, J. Hazard. Mater., 176, 692 (2010); doi:10.1016/j.jhazmat.2009.11.087.
H. Wackerbarth, C. Salb, L. Gundrum, M. Niederkruger, K. Christou, V. Beushausen and W. Viöl, Appl. Opt., 49, 4362 (2010); doi:10.1364/AO.49.004362.
M.R. Leahy-Hoppa, M.J. Fitch and R. Osiander, Anal. Bioanal. Chem., 395, 247 (2009); doi:10.1007/s00216-009-2803-z.
K. Wells and D.A. Bradley, Appl. Radiat. Isot., 70, 1729 (2012); doi:10.1016/j.apradiso.2012.01.011.
D.R. Shankaran, K.V. Gobi, T. Sakai, K. Matsumoto, K. Toko and N. Miura, Biosens. Bioelectron., 20, 1750 (2005); doi:10.1016/j.bios.2004.06.044.
E.S. Bromage, T. Lackie, M.A. Unger, J. Ye and S.L. Kaattari, Biosens. Bioelectron., 22, 2532 (2007); doi:10.1016/j.bios.2006.10.001.
M. Altstein, A. Bronshtein, B. Glattstein, A. Zeichner, T. Tamiri and J. Almog, Anal. Chem., 73, 2461 (2001); doi:10.1021/ac001376y.
Y. Mizuta, T. Onodera, P. Singh, K. Matsumoto, N. Miura and K. Toko, Biosens. Bioelectron., 24, 191 (2008); doi:10.1016/j.bios.2008.03.042.
G.V. Zyryanov, M.A. Palacios and P. Anzenbacher Jr., Org. Lett., 10, 3681 (2008); doi:10.1021/ol801030u.
S. Girotti, S. Eremin, A. Montoya, M.J. Moreno, P. Caputo, M. D'Elia, L. Ripani, F.S. Romolo and E. Maiolini, Anal. Bioanal. Chem., 396, 687 (2010); doi:10.1007/s00216-009-3264-0.
L. Feng, H. Li, Y. Qu and C. Lu, Chem. Commun., 48, 4633 (2012);doi:10.1039/C2CC16115J.
C.M. Maragos, Mycotoxin Res., 22, 96 (2006); doi:10.1007/BF02956771.
G.P. Anderson and E.R. Goldman, J. Immunol. Methods, 339, 47 (2008); doi:10.1016/j.jim.2008.08.001.