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Vol. 26 No. 2 (2014): Vol 26 Issue 2

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Spectrophotometric Determination of Tiopronin in Pharmaceutical Samples by Phosphorus Molybdenum Blue

https://doi.org/10.14233/ajchem.2014.15375
Binglin Fan1
1College of Chemistry and Environmental Science, Henan Normal University, Key Laboratory of Environmental Pollution Control Technology of Henan Province, Xinxiang 453007, Henan Province, P.R. China 2School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, Henan Province, P.R. China *Corresponding authors: Fax: +86 373 3326336; Tel: +86 373 3326335; E-mail: mercury6068@hotmail.com; binglinfan2006@126.com
Yingling Wang2
1College of Chemistry and Environmental Science, Henan Normal University, Key Laboratory of Environmental Pollution Control Technology of Henan Province, Xinxiang 453007, Henan Province, P.R. China 2School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, Henan Province, P.R. China *Corresponding authors: Fax: +86 373 3326336; Tel: +86 373 3326335; E-mail: mercury6068@hotmail.com; binglinfan2006@126.com
Jie Yang2
1College of Chemistry and Environmental Science, Henan Normal University, Key Laboratory of Environmental Pollution Control Technology of Henan Province, Xinxiang 453007, Henan Province, P.R. China 2School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, Henan Province, P.R. China *Corresponding authors: Fax: +86 373 3326336; Tel: +86 373 3326335; E-mail: mercury6068@hotmail.com; binglinfan2006@126.com
Tianjun Ni2
1College of Chemistry and Environmental Science, Henan Normal University, Key Laboratory of Environmental Pollution Control Technology of Henan Province, Xinxiang 453007, Henan Province, P.R. China 2School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, Henan Province, P.R. China *Corresponding authors: Fax: +86 373 3326336; Tel: +86 373 3326335; E-mail: mercury6068@hotmail.com; binglinfan2006@126.com
Quanmin Li1
1College of Chemistry and Environmental Science, Henan Normal University, Key Laboratory of Environmental Pollution Control Technology of Henan Province, Xinxiang 453007, Henan Province, P.R. China 2School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, Henan Province, P.R. China *Corresponding authors: Fax: +86 373 3326336; Tel: +86 373 3326335; E-mail: mercury6068@hotmail.com; binglinfan2006@126.com

Asian Journal of Chemistry, Vol. 26 No. 2 (2014): Vol 26 Issue 2

Abstract

A novel method is established for spectrophotometric determination of tiopronin by phosphorus molybdenum blue. It is based on the fact that PO43- reacts with Mo7O246- in 0.47 mol/L H2SO4 solution to form a product of phosphorus-molybdenum heteropoly acid ([H2PMo12O40]–) which is reduced to phosphorus molybdenum blue (H3PO4·10MoO3·Mo2O5) by tiopronin. The absorbance of phosphorus molybdenum blue is measured at the absorption maximum of 730 nm and the amount of tiopronin can be determined based on this absorbance. Absorbance is linear with the concentration of tiopronin in the range of 1.6-40 μg/mL and the regression equation is A = -0.1193 + 0.01927c (μg/mL) with a correlation coefficient of 0.9989 and the apparent molar absorption coefficient of 3.14 × 103 L/(mol cm). The detection limit and the RSD are 1 μg/mL and 0.92 %, respectively. Thus, the method is validated and commonly available to determine tiopronin in pharmaceutical samples.

Keywords

Phosphorus molybdenum blue Tiopronin Spectrophotometry.
Fan1, B., Wang2, Y., Yang2, J., Ni2, T., & Li1, Q. (2014). Spectrophotometric Determination of Tiopronin in Pharmaceutical Samples by Phosphorus Molybdenum Blue. Asian Journal of Chemistry, 26(2), 331–334. https://doi.org/10.14233/ajchem.2014.15375
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