HPLC Method for Simultaneous Analysis of Ranitidine and Metronidazole in Dosage Forms

Loti David King'ori; Roderick B. Walker*

doi:10.14233/ajchem.2014.15432

Issue

Vol. 26 No. 2 (2014): Vol 26 Issue 2

Issue Published : January 15, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

HPLC Method for Simultaneous Analysis of Ranitidine and Metronidazole in Dosage Forms

https://doi.org/10.14233/ajchem.2014.15432

Loti David King'ori

Division of Pharmaceutics, Faculty of Pharmacy, Rhodes University, Grahamstown 6140, South Africa *Corresponding author: Fax: +27 46 603 7506; Tel: +27 46 603 8381; E-mail: R.B.Walker@ru.ac.za

Roderick B. Walker*

Division of Pharmaceutics, Faculty of Pharmacy, Rhodes University, Grahamstown 6140, South Africa *Corresponding author: Fax: +27 46 603 7506; Tel: +27 46 603 8381; E-mail: R.B.Walker@ru.ac.za

Asian Journal of Chemistry, Vol. 26 No. 2 (2014): Vol 26 Issue 2
Article Published : January 16, 2014

Abstract

A simple, rapid, precise and accurate stability indicating HPLC method for the simultaneous analysis of metronidazole and ranitidine in dosage forms has been developed and validated. Calibration curves for metronidazole and ranitidine exhibited linearity (R² = 0.9995 for both compounds) over the concentration ranges investigated. The method was sensitive, selective and accurate for both compounds. Both drugs were found to be stable following acid hydrolysis studies. However, following alkali hydrolysis degradation of both compounds was observed. Furthermore metronidazole appeared to be stable following oxidative studies however ranitidine underwent complete degradation under these conditions. Both drugs were well resolved from the degradation products. The stability indicating chromatographic method has the necessary precision and accuracy for the simultaneous analysis of metronidazole and ranitidine in dosage forms.

Keywords

Metronidazole Ranitidine HPLC Stability-indicating Validation.

David King’ori, L., & B. Walker*, R. (2014). HPLC Method for Simultaneous Analysis of Ranitidine and Metronidazole in Dosage Forms. Asian Journal of Chemistry, 26(2), 426–430. https://doi.org/10.14233/ajchem.2014.15432

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References

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References

R.L. Snyder, J.J. Kirkland and W.J. Dolan, Introduction to Modern Liquid Chromatography, John Wiley & Sons, United States of America, edn 3, Vol. 1 (2010)..

T.G. do Nascimento, E. de Jesus Oliveira and R.O. Macédo, J. Pharm. Biomed. Anal., 37, 777 (2005); doi:10.1016/j.jpba.2004.11.042.

J.S. Munro and T.A. Walker, J. Chromatogr. A, 914, 13 (2001); doi:10.1016/S0021-9673(01)00540-4.

G.A. Shabir, J. Chromatogr. A, 987, 57 (2003); doi:10.1016/S0021-9673(02)01536-4.

C. Flores Pérez, H. Juárez Olguín, J. Flores Pérez, A. Toledo López, I. Lares Asseff and C. Alvarez García, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 795, 141 (2003); doi:10.1016/S1570-0232(03)00474-4.

G. Lunn and R.N. Schmuff, Ranitidine: In HPLC Methods for Pharmaceutical Analysis John Wiley & Sons, United States of America, p. 1209 (2000)..

M. Bakshi, B. Singh, A. Singh and S. Singh, J. Pharmaceut. Biomed.Anal., 26, 891 (2001); doi:10.1016/S0731-7085(01)00475-7.

G. Lunn, Metronidazole: In HPLC Methods for Pharmaceutical Analysis John Wiley & Sons, United States of America, pp. 1354-1362 (2000).

R.B. Shah, P.R. Hullahalli, M.A. Tawakkul, P.J. Faustino, A. Nguyenpho and M.A. Khan, Clin. Res. Regul. Aff., 23, 35 (2006); doi:10.1080/10601330500545556.

M. Bakshi and S. Singh, Pharm. Technol., 27, 148 (2003).

K.I. Baren, Drugs of Acid Related Disorders. In South Africa Medicine Formulary, 8th[A02], Health and Medical Publishing Group: Cape Town, pp. 39-44 (2008).

J.Q. Huang and R.H. Hunt, Best Pract. Res.Cl. Ga., 15, 355 (2001); doi:10.1053/bega.2001.0184.

D. Clark and J. Strandell, Eur. J. Clin. Pharmacol., 62, 473 (2006); doi:10.1007/s00228-006-0131-1.

S. Dial, J.A. Delaney, A.N. Barkun and S. Suissa, JAMA, 294, 2989 (2005); doi:10.1001/jama.294.23.2989.

C. Scarpignato, Drug Discov. Today Ther. Strateg., 4, 155 (2007); doi:10.1016/j.ddstr.2007.09.003.

J.P. Gisbert, L. Gonzalez, X. Calvet, M. Roque, R. Gabriel and J.M. Pajares, Aliment. Pharmacol. Ther., 15, 917 (2001); doi:10.1046/j.1365-2036.2001.01012.x.

M.S. Khuroo, K.L. Farhat and I.E. Kageyi, Gastroenterol. Hepatol., 20, 11 (2005).

Sung, Leung, Ling, Yung, Chan, Lee, Cheng and Chung, Aliment. Pharmacol. Ther., 12, 725 (1998); doi:10.1046/j.1365-2036.1998.00367.x.

C. Melikian, T.J. White, A. Vanderplas, C.M. Dezii and E. Chang, Clin. Ther., 24, 460 (2002); doi:10.1016/S0149-2918(02)85047-0.

R.L. Snyder, J.J. Kirkland and L.J. Glajch, Practical HPLC Method Development., John Wiley and Sons, USA, edn 2 (1997)..

M. Bakshi and S. Singh, J. Pharm. Biomed. Anal., 28, 1011 (2002); doi:10.1016/S0731-7085(02)00047-X.

Issue

Vol. 26 No. 2 (2014): Vol 26 Issue 2

HPLC Method for Simultaneous Analysis of Ranitidine and Metronidazole in Dosage Forms

Abstract

Keywords

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