Issue

Vol. 26 No. 1 (2014): Vol 26 Issue 1

Copyright (c) 2014 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Isolation and Characterization of Pseudomonas sp. nai8 Capable of Naphthalene Degradation

https://doi.org/10.14233/ajchem.2014.15365
Zhiqiang Cai
Lab. of Applied Microbiology, Key Lab. of Drug Manufacturing and Quality Control Engineering of Changzhou, Changzhou University, Changzhou 213164, P.R. China
Sai Shi
Lab. of Applied Microbiology, Key Lab. of Drug Manufacturing and Quality Control Engineering of Changzhou, Changzhou University, Changzhou 213164, P.R. China
Shanshan Li
Lab. of Applied Microbiology, Key Lab. of Drug Manufacturing and Quality Control Engineering of Changzhou, Changzhou University, Changzhou 213164, P.R. China
Wenjie Zhang
Lab. of Applied Microbiology, Key Lab. of Drug Manufacturing and Quality Control Engineering of Changzhou, Changzhou University, Changzhou 213164, P.R. China
Jinyan Cai
Lab. of Applied Microbiology, Key Lab. of Drug Manufacturing and Quality Control Engineering of Changzhou, Changzhou University, Changzhou 213164, P.R. China
Guanghua Yang
Lab. of Applied Microbiology, Key Lab. of Drug Manufacturing and Quality Control Engineering of Changzhou, Changzhou University, Changzhou 213164, P.R. China
Xiaolin Zhu
Lab. of Applied Microbiology, Key Lab. of Drug Manufacturing and Quality Control Engineering of Changzhou, Changzhou University, Changzhou 213164, P.R. China

Corresponding Author(s) : Zhiqiang Cai

zhqcai@hotmail.com

Asian Journal of Chemistry, Vol. 26 No. 1 (2014): Vol 26 Issue 1

Abstract

Using the base mineral medium containing 800 mg/L of naphthalene, a degrading bacteria with higher degradation activity was isolated and identified as Pseudomonas sp. based on physiological, biochemical characteristics and 16s rDNA sequence. The effects of Pseudomonas sp. Nai8’s degrading ability, e.g., naphthalene concentration, temperature and pH, were investigated. The results showed that under the condition of 30 ºC, pH 7.0 and concentration of naphthalene of 1000 mg/L, the degradation rate in 48 and 96 h was 70.4 and 96.2 %, respectively. Pseudomonas sp. Nai8 could also degrade other aromatic hydrocarbons, such as dimethylbenzene, phenol, benzyl acid, utilizing each of them as sole carbon and energy source for its growth, which showed the strain Nai8 has good biodegradation diversity. The kinetics of naphthalene degradation by Nai8 was also calculated and the naphthalene biodegradation constant (k) was 0.038 h-1 with R2 value 0.9679, which showed that the strain nai8 could degrade naphthalene remarkably.

Keywords

Pseudomonas Naphthalene Biodegradation Kinetics Characterization
Cai, Z., Shi, S., Li, S., Zhang, W., Cai, J., Yang, G., & Zhu, X. (2013). Isolation and Characterization of Pseudomonas sp. nai8 Capable of Naphthalene Degradation. Asian Journal of Chemistry, 26(1), 164–168. https://doi.org/10.14233/ajchem.2014.15365
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. P. Nigam, I.M. Banat, R. Marchant and D. Singh, Environ. Int., 24, 671 (1998); doi:10.1016/S0160-4120(98)00037-3.
  2. E.C. Santos, R.J.S. Jacques, F.M. Bento, M.C.R. Peralba, P.A. Selbach, E.L.S. Sá and F.A.O. Camargo, Bioresour. Technol., 99, 2644 (2008); doi:10.1016/j.biortech.2007.04.050.
  3. F.J. Rivas, J. Hazard. Mater., 138, 234 (2006); doi:10.1016/j.jhazmat.2006.07.048.
  4. K.T. Semple, K.J. Doick, L.Y. Wick and H. Harms, Environ. Pollut., 150, 166 (2007); doi:10.1016/j.envpol.2007.07.023.
  5. Z. Cai, Q. Chen, H. Wang, Y. He, W. Wang, X. Zhao and Q. Ye, Appl. Microbiol. Biotechnol., 96, 1371 (2012); doi:10.1007/s00253-011-3867-1.
  6. G. Yang, Y. He, Z. Cai, X. Zhao, L. Wang and L. Wang, Afr. J. Biotechnol., 10, 10456 (2011).
  7. C. Calvo, F.L. Toledo and J. González-López, J. Biotechnol., 109, 255 (2004); doi:10.1016/j.jbiotec.2004.01.009.
  8. G. Hwang, S.R. Park, C.H. Lee, I.S. Ahn, Y.J. Yoon and B.J. Mhin, J. Hazard. Mater., 172, 491 (2009); doi:10.1016/j.jhazmat.2009.07.009.
  9. Y. Wang, J. Xin, H. Song, J. Zhang and C. Xia, China Biotechnol., 29, 119 (2009).
  10. M. Zeinali, M. Vossoughi and S.K. Ardestani, Chemosphere, 72, 905 (2008); doi:10.1016/j.chemosphere.2008.03.038.
  11. Z. Cai, L. Huang, Y. He, S. Shi, X. Zhao and L. Wang, Biol. Environ., 3, 293 (2012).
  12. Z. Cai, X. Xu, Q. Chen, X. Zhao, H. Wang, L. Huang and Q. Ye, J. Chem. Eng. Chinese U., 26, 93 (2012).
  13. X. Dong and M. Cai, “Manual of Bacteria Identify”. Science Press, Beijing, 2001.
  14. P. Buchanan, H.W. Schubert and J.K. Holt, “Bergey’s Manual of Determinative Bacteriology”. 9th [M]. The Williams and Wilklins Company, Beltimore, 1984.
  15. L. Ayed, K. Chaieb, A. Cheref and A. Bakhrouf, World J. Microbiol. Biotechnol., 25, 705 (2009); doi:10.1007/s11274-008-9941-x.
  16. T. Hadibarata, A.R.M. Yusoff, A. Aris and R.A. Kristanti, J. Environ. Sci., 24, 728 (2012); doi:10.1016/S1001-0742(11)60843-7.
  17. C. Lin, L. Gan and Z. Chen, J. Hazard. Mater., 182, 771 (2010); doi:10.1016/j.jhazmat.2010.06.101.
  18. V. Patel, S. Jain and D. Madamwar, Bioresour. Technol., 107, 122 (2012); doi:10.1016/j.biortech.2011.12.056.
  19. S.M. Bamforth and I. Singleton, J. Chem. Technol. Biotechnol., 80, 723 (2005); doi:10.1002/jctb.1276.
  20. J.L. Chen, M.H. Wong, Y.S. Wong and N.F.Y. Tam, Mar. Pollut. Bull., 57, 695 (2008); doi:10.1016/j.marpolbul.2008.03.013.
  21. B. Morasch, E. Annweiler, R.J. Warthmann and R.U. Meckenstock, J. Microbiol. Methods, 44, 183 (2001); doi:10.1016/S0167-7012(00)00242-6.
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 0 Download : 0