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Vol. 26 No. 1 (2014): Vol 26 Issue 1

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Investigation on the Antioxidant Activity of Roots and Stem of Colchicum turcicum L.

https://doi.org/10.14233/ajchem.2014.15160
Ismail Kili&ccedil
Faculty of Education, Science Section, Trakya University, Edirne, Turkey
Yesim Yesiloglu
Department of Chemistry, Faculty of Science, Trakya University, Edirne, Turkey
Yüksel Bayrak
Department of Chemistry, Faculty of Science, Trakya University, Edirne, Turkey

Corresponding Author(s) : Yesim Yesiloglu

yesimyesiloglu@trakya.edu.tr

Asian Journal of Chemistry, Vol. 26 No. 1 (2014): Vol 26 Issue 1

Abstract

Water and acetone extracts from Colchicum turcicum L. were investigated for their antioxidant and radical scavenging activities in four different assays, namely, total antioxidant activity, 1,1-dipenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, hydrogen peroxide scavenging and ferrous chelating activities. Antioxidant activity, ferrous chelating and hydrogen peroxide scavenging activities of C. turcicum extracts showed dose dependence and increased with concentration of C. turcicum extract. At the same concentration (100 μg/mL), water extract of C. turcicum (WECT) and acetone extract of C. turcicum (AECT) showed 53 and 48 % inhibition of lipid peroxidation of linoleic acid emulsion, respectively. On the other hand, butylated hydroxyanisole, butylated hydroxytoluene and ascorbic acid indicated inhibitions of 77, 71 and 59 % on the peroxidation of linoleic acid emulsion, respectively, at 50 μg/mL. Also, the total phenolic content in both water extract of C. turcicum and acetone extract of C. turcicum were determined as catechol equivalents. The total phenolics content of water and acetone extracts were determined by the Folin-Ciocalteu procedure and 0.454 and 2.172 mg catechol equivalent of phenols was detected in 1 g water extract of C. turcicum and acetone extract of C. turcicum.

Keywords

Colchicum turcicum L. Antioxidant activity Total phenol content Total flavonoids DPPH
Kili&ccedil, I., Yesiloglu, Y., & Bayrak, Y. (2013). Investigation on the Antioxidant Activity of Roots and Stem of Colchicum turcicum L. Asian Journal of Chemistry, 26(1), 5–9. https://doi.org/10.14233/ajchem.2014.15160
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