Copyright (c) 2014 Paweena Porrawatkul*, Rungnapa Pimsen
This work is licensed under a Creative Commons Attribution 4.0 International License.
Antioxidant Activity of Ethanolic Extract in Different Parts of Nutmeg (Myristica fragrants)
Corresponding Author(s) : Paweena Porrawatkul*
Asian Journal of Chemistry,
Vol 26 No Supplementary Issue
Abstract
Myristica fragrants (nutmeg) is a tropical fruit in Banda Island. It is also used to cultivate in the South of Thailand. It is used as conventional medicine for disease prevention and treatment. However, there are no research studies about its antioxidant activities using ethanol solvent. The total phenolic content and antioxidant capacity in different parts of ethanolic extract (pulp, mace and seed) from nutmeg were evaluated. Dried samples were extracted using 95 % ethanol, while Folin-Ciocalteu assay was employed to determine total phenolic content. Whereas, antioxidant activities were determined by ferric reducing antioxidant power and DPPH assay. The results revealed that the nutmeg seed contained the highest total phenolic content, which was 465.91 ± 03 mg GAE/100 g DW. For antioxidant activity, the nutmeg seed possessed the highest inhibited DPPH (IC50 = 4.6 ± 15 mg/L) and the highest ferric reducing antioxidant power values (542.39 ± 22 μmol TE/100 gDW), which were significantly higher than reference standard. Phenolic compounds in nutmeg samples have exhibited strong correlation with antioxidant capacity. Therefore, nutmeg, especially nutmeg seed is potentially functional food with high antioxidants.
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- O. Bamidele, A.M. Akinnuga, I.A. Alagbonsi, O.A. Ojo, J.O. Olorunfemi and M.A. Akuyoma, Int. J. Med. Medical Sci., 3, 215 (2011).
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- E.A. Weiss, Spice Crops, CAB international, USA (2002).
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- B. Vittaya, Encyclopaedia (Thai-China definition), vol. 1, p. 180 (2011).
- T.K. Lim, Edible Medicinal and Non-Medicinal Plants: Volume 3, Fruits, Springer, p. 583 (2012).
- R. Somani, S. Karve, D. Jain, K. Jain and A.K. Singhai, Pharmacog. Rev., 2, 68 (2008).
- B. Sultana, F. Anwar and M. Ashraf, Molecules, 14, 2167 (2009).
- B. Fuhrman and M. Aviram, J. Nutr., 136, 2273 (2006).
- P. Mahakunakorn, M. Tohda, Y. Murakami, K. Matsumoto and H. Watanabe, Biol. Pharm. Bull., 27, 38 (2004).
- I.F.F. Benzie and J.J. Strain, Anal. Biochem., 239, 70 (1996).
- Association of Official Analytical Chemists, Washington D.C., edn. 15 (1990).
- K.P. Tan, H.E. Khoo and A. Azrina, Int. Food Res. J., 20, 1049 (2013).
- A.D. Gupta and D. Rajpurohit, in eds.: V.R. Preedy, R.R. Watson and V. B. Patel, Antioxidant and antimicrobial activity of nutmeg (Myristica fragrans), In: Nuts & Seeds in Health and Disease Prevention, Elsevier Inc., Ch. 1, pp. 831-839 (2011).
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References
O. Bamidele, A.M. Akinnuga, I.A. Alagbonsi, O.A. Ojo, J.O. Olorunfemi and M.A. Akuyoma, Int. J. Med. Medical Sci., 3, 215 (2011).
R.N. Chopra and S.L. Nayer, Glossary of Indian Medicinal Plants, Council of Scientific and Industrial Research, New Delhi, edn. 3, p. 246 (1992).
M.A. Lyengar, Study of Crude Drugs, College of Pharmaceutical Science, Manipal, India, edn. 2, pp. 13-78 (1985).
S.B. Harborne and H. Baxter, A Handbook of Bioactive Compounds from Plants, Taylor and Francis, London (1995).
E.A. Weiss, Spice Crops, CAB international, USA (2002).
R. Nijsiri and M. Thawatchai, Thailand Herbs, edn. 2, vol. 1, p. 90 (2004).
B. Vittaya, Encyclopaedia (Thai-China definition), vol. 1, p. 180 (2011).
T.K. Lim, Edible Medicinal and Non-Medicinal Plants: Volume 3, Fruits, Springer, p. 583 (2012).
R. Somani, S. Karve, D. Jain, K. Jain and A.K. Singhai, Pharmacog. Rev., 2, 68 (2008).
B. Sultana, F. Anwar and M. Ashraf, Molecules, 14, 2167 (2009).
B. Fuhrman and M. Aviram, J. Nutr., 136, 2273 (2006).
P. Mahakunakorn, M. Tohda, Y. Murakami, K. Matsumoto and H. Watanabe, Biol. Pharm. Bull., 27, 38 (2004).
I.F.F. Benzie and J.J. Strain, Anal. Biochem., 239, 70 (1996).
Association of Official Analytical Chemists, Washington D.C., edn. 15 (1990).
K.P. Tan, H.E. Khoo and A. Azrina, Int. Food Res. J., 20, 1049 (2013).
A.D. Gupta and D. Rajpurohit, in eds.: V.R. Preedy, R.R. Watson and V. B. Patel, Antioxidant and antimicrobial activity of nutmeg (Myristica fragrans), In: Nuts & Seeds in Health and Disease Prevention, Elsevier Inc., Ch. 1, pp. 831-839 (2011).
B. Shan, Y.Z. Cai, M. Sun and H. Corke, J. Agric. Food Chem., 53, 7749 (2005).
D. Sreeramulu and M. Raghunath, Food Res. Int., 43, 1017 (2010).
S. Nair and R. Nagar, J. Assoc. Physicians of India, 46, 708 (1998).