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Copyright (c) 2014 Huina Zhao, Kai Cai, Bo Lei*, Zhu Ren, Bin Cai, Wenjie Pan*
This work is licensed under a Creative Commons Attribution 4.0 International License.
Development of Method to Determine C6-C3 Phenolic Acids in Tobacco (Nicotiana tabacum) by HPLC
Corresponding Author(s) : Huina Zhao
Asian Journal of Chemistry,
Vol. 26 No. 24 (2014)
Abstract
A method to simultaneous determination of four C6-C3 phenolic acids in tobacco by high performance liquid chromatography (HPLC) was established. Four C6-C3 phenolic acids were well separated in 40 min. The proposed method had high sensitivity, accuracy and repeatability with good linear relationship (R2 ³ 0.9992) when the concentration of phenolic acids was between 1.113 and 102.332 μg/mL. This method was applied to tobacco samples. The results showed that the content of four C6-C3 phenolic acids was significantly different in tobacco at different regions and growth stages, and the contents of p-coumaric acid, ferulic acid and sinapic acid in tobacco were influenced by the ecological conditions more than growth stages. This might be one of the reasons that the tobacco leaves in different regions had different quality.
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- E. Anouar, P. Košinová, D. Kozlowski, R. Mokrini, J.L. Duroux and P. Trouillas, Chem. Chem. Phys., 11, 7659 (2009).
- S.B. Revanappa and P.V. Salimath, J. Food Biochem., 35, 759 (2011).
- K.H. Kim, R. Tsao, R. Yang and S.W. Cui, Food Chem., 95, 466 (2006).
- U. Gawlik-Dziki, M. Swieca and D. Dziki, J. Agric. Food Chem., 60, 4603 (2012).
- A. Caniova and E. Brandsteterova, Chem. Anal. (Warsaw), 46, 757 (2001).
- K. Aaby, D. Ekeberg and G. Skrede, J. Agric. Food Chem., 55, 4395 (2007).
- P. Mattila and J. Kumpulainen, J. Agric. Food Chem., 50, 3660 (2002).
- C. del Mar Verde Méndez, M.Á. Rodríguez Delgado, E.M. Rodríguez Rodríguez and C. Díaz Romero, J. Agric. Food Chem., 52, 1323 (2004).
- D.L. Luthria, S. Mukhopadhyay and D. Krizek, J. Food Compos. Anal., 19, 771 (2006).
- B. Dimitrova, R. Gevrenova and E. Anklam, Phytochem. Anal., 18, 24 (2007).
- K. Pyrzynska and M. Biesaga, Trends Analyt. Chem., 28, 893 (2009).
- J. Lachman, A. Hejtmánkova, J. Sykora, J. Karban, M. Orsák and B. Rygerová, Czech J. Food Sci., 28, 412 (2010).
- S. Qiao, R. Shi, M. Liu, C. Zhang, W. Yang, X. Shi, X. Jiang, C. Wang and Q. Wang, Food Chem., 129, 1297 (2011).
- Z.C. Zhang, M. Xu, S.F. Sun, X. Qiao, B.R. Wang, J. Han and D.A. Guo, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 871, 7 (2008).
- A. Olkowski, R. Amarowicz, P. Yu, J.J. McKinnon and D.D. Maenz, Pol. J. Food Nutr. Sci., 53, 53 (2003).
- M. Nikolova and R. Gevrenova, Pharm. Biol., 43, 289 (2005).
- P.A. Kroon and G. Williamson, Biotechnol. Appl. Biochem., 23, 263 (1996).
- R. Amarowicz and P.P. Kolodziejczyk, Nahrung, 45, 62 (2001).
- M.F. Andreasen, L.P. Christensen, A.S. Meyer and Hansen, J. Sci. Food Agric., 79, 411 (1999).
- L. Barros, M. Dueñas, I.C.F.R. Ferreira, P. Baptista and C. Santos-Buelga, Food Chem. Toxicol., 47, 1076 (2009).
- K. Saito and F. Matsuda, Annu. Rev. Plant Biol., 61, 463 (2010).
References
E. Anouar, P. Košinová, D. Kozlowski, R. Mokrini, J.L. Duroux and P. Trouillas, Chem. Chem. Phys., 11, 7659 (2009).
S.B. Revanappa and P.V. Salimath, J. Food Biochem., 35, 759 (2011).
K.H. Kim, R. Tsao, R. Yang and S.W. Cui, Food Chem., 95, 466 (2006).
U. Gawlik-Dziki, M. Swieca and D. Dziki, J. Agric. Food Chem., 60, 4603 (2012).
A. Caniova and E. Brandsteterova, Chem. Anal. (Warsaw), 46, 757 (2001).
K. Aaby, D. Ekeberg and G. Skrede, J. Agric. Food Chem., 55, 4395 (2007).
P. Mattila and J. Kumpulainen, J. Agric. Food Chem., 50, 3660 (2002).
C. del Mar Verde Méndez, M.Á. Rodríguez Delgado, E.M. Rodríguez Rodríguez and C. Díaz Romero, J. Agric. Food Chem., 52, 1323 (2004).
D.L. Luthria, S. Mukhopadhyay and D. Krizek, J. Food Compos. Anal., 19, 771 (2006).
B. Dimitrova, R. Gevrenova and E. Anklam, Phytochem. Anal., 18, 24 (2007).
K. Pyrzynska and M. Biesaga, Trends Analyt. Chem., 28, 893 (2009).
J. Lachman, A. Hejtmánkova, J. Sykora, J. Karban, M. Orsák and B. Rygerová, Czech J. Food Sci., 28, 412 (2010).
S. Qiao, R. Shi, M. Liu, C. Zhang, W. Yang, X. Shi, X. Jiang, C. Wang and Q. Wang, Food Chem., 129, 1297 (2011).
Z.C. Zhang, M. Xu, S.F. Sun, X. Qiao, B.R. Wang, J. Han and D.A. Guo, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 871, 7 (2008).
A. Olkowski, R. Amarowicz, P. Yu, J.J. McKinnon and D.D. Maenz, Pol. J. Food Nutr. Sci., 53, 53 (2003).
M. Nikolova and R. Gevrenova, Pharm. Biol., 43, 289 (2005).
P.A. Kroon and G. Williamson, Biotechnol. Appl. Biochem., 23, 263 (1996).
R. Amarowicz and P.P. Kolodziejczyk, Nahrung, 45, 62 (2001).
M.F. Andreasen, L.P. Christensen, A.S. Meyer and Hansen, J. Sci. Food Agric., 79, 411 (1999).
L. Barros, M. Dueñas, I.C.F.R. Ferreira, P. Baptista and C. Santos-Buelga, Food Chem. Toxicol., 47, 1076 (2009).
K. Saito and F. Matsuda, Annu. Rev. Plant Biol., 61, 463 (2010).