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Vol. 26 No. 24 (2014)

Copyright (c) 2014 Li Ma1, Han-Jing Zhu1, Cheng-Cheng Yao1, Kui Lu1

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Synthesis and Properties of Enkephalin Oligonucleotide Conjugates

https://doi.org/10.14233/ajchem.2014.18325
Li Ma1
1School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, P.R. China 2School of Materials and Chemical Engineering, Henan Institute of Engineering, Zhengzhou 451191, P.R. China *Corresponding author: Tel: +86 15803885965; E-mail: luckyluke1106@163.com
Han-Jing Zhu1
1School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, P.R. China 2School of Materials and Chemical Engineering, Henan Institute of Engineering, Zhengzhou 451191, P.R. China *Corresponding author: Tel: +86 15803885965; E-mail: luckyluke1106@163.com
Cheng-Cheng Yao1
1School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, P.R. China 2School of Materials and Chemical Engineering, Henan Institute of Engineering, Zhengzhou 451191, P.R. China *Corresponding author: Tel: +86 15803885965; E-mail: luckyluke1106@163.com
Kui Lu1
1School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, P.R. China 2School of Materials and Chemical Engineering, Henan Institute of Engineering, Zhengzhou 451191, P.R. China *Corresponding author: Tel: +86 15803885965; E-mail: luckyluke1106@163.com

Corresponding Author(s) : Li Ma1

Asian Journal of Chemistry, Vol. 26 No. 24 (2014)

Abstract

Peptide-oligonucleotide conjugates (POCs) is a kind of potential gene therapy drugs. It has several applications, including improving antisense agents for interfering with the RNA function and targeting disease-causing mRNA within cells. In this paper, an efficient synthesis of six enkephalin-oligonucleotide conjugates had been accomplished by azide-alkyne cycloaddition, in which the oligonucleotide and peptides were directly appended by azide and alkyne groups. Meanwhile, the hydrolytic stability of enkephalin-oligonucleotide conjugates was studied by reversed phase HPLC and the interaction of the products with pUC18 DNA also conducted by agarose gel electrophoresis. The results obtained showed that the triazole linkage is much more stable in slightly acidic environment than in slightly alkaline, the interaction between enkephalin-oligonucleotide conjugates and pUC18DNA is stronger than that between enkephalin and pUC18 DNA.

Keywords

Enkephalin Oligonucleotide Conjugates Synthesis.
Ma1, L., Zhu1, H.-J., Yao1, C.-C., & Lu1, K. (2014). Synthesis and Properties of Enkephalin Oligonucleotide Conjugates. Asian Journal of Chemistry, 26(24), 8547–8552. https://doi.org/10.14233/ajchem.2014.18325
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