Issue

Vol. 26 No. 24 (2014)

Copyright (c) 2014 Masoom Raza Siddiqui1, Saikh Mohammad Wabaidur1, Zeid A. Alothman1, Moonis Ali Khan1, Mohammad Shamsul Ola2

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Quantitative Assessment of Cefuroxime: A Second Generation Cephalosporin in Pharmaceutical Formulations and Spiked Plasma Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

https://doi.org/10.14233/ajchem.2014.18060
Masoom Raza Siddiqui1
1Advanced Materials Research Chair, Chemistry Department, College of Science, King Saud University, Riyadh , Saudi Arabia 2Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia *Corresponding author: Fax: +966 14675992; Tel: +966 14674198; E-mail: tarabai22@yahoo.com.sg
Saikh Mohammad Wabaidur1
1Advanced Materials Research Chair, Chemistry Department, College of Science, King Saud University, Riyadh , Saudi Arabia 2Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia *Corresponding author: Fax: +966 14675992; Tel: +966 14674198; E-mail: tarabai22@yahoo.com.sg
Zeid A. Alothman1
1Advanced Materials Research Chair, Chemistry Department, College of Science, King Saud University, Riyadh , Saudi Arabia 2Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia *Corresponding author: Fax: +966 14675992; Tel: +966 14674198; E-mail: tarabai22@yahoo.com.sg
Moonis Ali Khan1
1Advanced Materials Research Chair, Chemistry Department, College of Science, King Saud University, Riyadh , Saudi Arabia 2Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia *Corresponding author: Fax: +966 14675992; Tel: +966 14674198; E-mail: tarabai22@yahoo.com.sg
Mohammad Shamsul Ola2
1Advanced Materials Research Chair, Chemistry Department, College of Science, King Saud University, Riyadh , Saudi Arabia 2Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia *Corresponding author: Fax: +966 14675992; Tel: +966 14674198; E-mail: tarabai22@yahoo.com.sg

Corresponding Author(s) : Masoom Raza Siddiqui1

Asian Journal of Chemistry, Vol. 26 No. 24 (2014)

Abstract

A simple and rapid method has been developed for the determination of cefuroxime in pharmaceutical formulations and spiked plasma. The method involves the application of ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the quantitative assessment of cefuroxime. The chromatographic separation of the cefuroxime was carried out using BEH C18 column (100 mm × 2.1 mm, 1.7 μm particle size) using a binary mobile phase mixture of 1-octane sulfonic acid sodium salt (5.78 × 10-4 M) and acetonitrile (50:50, v/v) at a flow rate of 0.5 mL min-1. The UPLC-MS/MS have shorter analysis time of 1 min was found to be linear over the concentration range of 0.5-5 μg mL-1. Limit of detection and limit of quantitation of the UPLC-MS/MS method was obtained to be 0.10 and 0.301 μg mL-1. The method was validated as per the ICH guidelines. The recovery studies suggest an excellent recovery of the procedure which was found in the range of 99.94-100.47 % for the pharmaceutical formulation and 95.63 to 96.89 %. for the spiked plasma. The RSD range of the developed analytical procedure ranged from 0.90 %-1.68 % in intra-day studies and 1.42 %-1.97 %, respectively.

Keywords

Cefuroxime Ultraperformance liquid chromatography-tandem mass spectrometry.
Raza Siddiqui1, M., Mohammad Wabaidur1, S., A. Alothman1, Z., Ali Khan1, M., & Shamsul Ola2, M. (2014). Quantitative Assessment of Cefuroxime: A Second Generation Cephalosporin in Pharmaceutical Formulations and Spiked Plasma Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry. Asian Journal of Chemistry, 26(24), 8479–8482. https://doi.org/10.14233/ajchem.2014.18060
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. C.R. Gelman, B.H. Rumakc and T.A. Hutchinson; Product Information, Ceftin®, Cefuroxime Axetil. Glaxo Inc., Research Triangle Park, NC, USA, In: L DRUGEX® System; Micromedex Inc.: Englewood, CO, USA (1999).
  2. C. Dollery, Therapeutic Drugs, Churchill Livingstone, London, U.K., edn 2 (1999).
  3. Y.Y. Zhang, P.J. Wu and Q. Zhang, Zhonghua NeiKe ZaZhi, 28, 340 (1989).
  4. M.S. Al-Said, K.I. Al-Khamis, E.M. Niazy, Y.M. El-Sayed, K.A. Al-Rashood, S. Al-Bella, M.A. Al-Yamani, A. Al-Najjar, S.M. Alam, R. Dham and Q. Zaman Qumaruzaman, Biopharm. Drug Dispos., 21, 205 (2000).
  5. http://reference.medscape.com/drug/ceftin-zinacef-cefuroxime-342500.
  6. M. Sasinoska-Motyl, I.W. Wisniewska and I. Gumulka, Acta Pol. Pharm., 52, 391 (1995).
  7. R.D. Foord, Antimicrob. Agents Chemother., 9, 741 (1976).
  8. M.R. Sengar, S.V. Gandhi, U.P. Patil and V.S. Rajmane, Int. J. Chem. Tech. Res., 1, 1105 (2009).
  9. A. Szlagowska, M. Kaza and P.J. Rudzki, Acta Polon. Pharm. Drug Res., 67, 677 (2010).
  10. G. Piva, D. Farin, I .Gozlan and R. Kitzes-Cohen, Chromatographia, 51, 154 (2000).
  11. P. Jain, M. Patel and S. Surana, Int. J. Drug Develop. Res., 3, 318 (2011).
  12. S. Shelk, S. Dongre, A. Rathi, D. Dhamecha, S. Maria and M.H.G. Dehghan, Asian J. Res. Chem., 2, 222 (2009).
  13. M.D. Game, D.M. Sakarkar, K.B. Gabhane and K.K. Tapar, Int. J. Chem. Tech. Res., 2, 1259 (2010).
  14. M.S. Elazazy and A. Shalaby, E-J. Chem., 9, 2261 (2012).
  15. G. Pajchel and S. Tyski, J. Chromatogr. A, 895, 27 (2000).
  16. K.R. Sireesha, D.V. Mhaske, S.S. Kadam and S.R. Dhaneshwar, Indian J. Pharm. Sci., 66, 278 (2004).
  17. C.-C. Mondea, L. Vlase, A. Gyéresi and M. Bojita, Farmacia, 58, 408 (2010).
  18. C.J. Briscoe, M.R. Stiles and D.S. Hage, J. Pharm. Biomed. Anal., 44, 484 (2007).
Read More
Author biographies is not available.
Download
Statistic
Read Counter : 0