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Vol. 26 No. 23 (2014)

Copyright (c) 2014 Ayla Demirci*, Emel Gevrek

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Optimization of Hollow Fiber Liquid-Phase Microextraction Combined with GC-ECD for Determination of Organochlorine Pesticides in Orange Juice

https://doi.org/10.14233/ajchem.2014.17910
Ayla Demirci*
Department of Chemistry, Kirikkale University, Kirikkale, Turkey *Corresponding author: Fax: +90 318 3572461; Tel: +90 318 3574242; E-mail: aydemirci55@gmail.com
Emel Gevrek
Department of Chemistry, Kirikkale University, Kirikkale, Turkey *Corresponding author: Fax: +90 318 3572461; Tel: +90 318 3574242; E-mail: aydemirci55@gmail.com

Corresponding Author(s) : Ayla Demirci*

Asian Journal of Chemistry, Vol. 26 No. 23 (2014)

Abstract

The hollow fiber protected liquid phase microextraction (HF-LPME) technique was evaluated for the extraction of organochlorine pesticides in orange juice prior to gas chromatography-electron capture detection (GC-ECD) analysis. At first, the effects of the HF-LPME experimental parameters on the enrichment factor were studied simultaneously using a 25–1 fractional factorial design. The HF-LPME variables of interest were extraction time, stirring speed, acceptor phase, salt (NaCl, w/v %) and pH. In the next step, a two-level plus centre point fractional factorial experiment was performed to evaluate the effects of three parameters, including the extraction time, stirring speed and pH of donor phase. The optimized HF-LPME conditions were as follows: an extraction time of 20 min, a stirring speed of 800 rpm, an acceptor phase of toluene, no salt and a source pH of 6. The extraction calibration plots were linear over the range of 0.25-10 ng mL–1 (r2: 0.9863-0.9995) and the limits of detection for ten pesticides studied were from 0.012 to 0.456 ng mL–1. Good enrichment factors were achieved (66-251-fold) with this method and good repeatabilities were obtained, with relative standard deviations below 19.9 %.

Keywords

Chlorinated pesticides Experimental design GC-ECD Hollow fiber microextraction Juice.
Demirci*, A., & Gevrek, E. (2014). Optimization of Hollow Fiber Liquid-Phase Microextraction Combined with GC-ECD for Determination of Organochlorine Pesticides in Orange Juice. Asian Journal of Chemistry, 26(23), 8197–8201. https://doi.org/10.14233/ajchem.2014.17910
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