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Vol. 26 No. 23 (2014)

Copyright (c) 2014 Xinquan Liang1, Xianhui Liang1, Huihuan He2, Jidong Liu1, Dengjun Lu1

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Determination of Monomer Phenols in Cane Juice by Reversed-Phase High-Performance Liquid Chromatography with UV Detection

https://doi.org/10.14233/ajchem.2014.17596
Xinquan Liang1
1Light Industry and Food Engineering Institute, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, P.R. China 2Guangxi Vocational & Technical Institute of Industry, 37 Xiuling Road, Nanning, Guangxi 530004, P.R. China *Corresponding author: Fax: +86 771 3231590; Tel: +86 771 3237329; E-mail: liuan6126@126.com
Xianhui Liang1
1Light Industry and Food Engineering Institute, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, P.R. China 2Guangxi Vocational & Technical Institute of Industry, 37 Xiuling Road, Nanning, Guangxi 530004, P.R. China *Corresponding author: Fax: +86 771 3231590; Tel: +86 771 3237329; E-mail: liuan6126@126.com
Huihuan He2
1Light Industry and Food Engineering Institute, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, P.R. China 2Guangxi Vocational & Technical Institute of Industry, 37 Xiuling Road, Nanning, Guangxi 530004, P.R. China *Corresponding author: Fax: +86 771 3231590; Tel: +86 771 3237329; E-mail: liuan6126@126.com
Jidong Liu1
1Light Industry and Food Engineering Institute, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, P.R. China 2Guangxi Vocational & Technical Institute of Industry, 37 Xiuling Road, Nanning, Guangxi 530004, P.R. China *Corresponding author: Fax: +86 771 3231590; Tel: +86 771 3237329; E-mail: liuan6126@126.com
Dengjun Lu1
1Light Industry and Food Engineering Institute, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, P.R. China 2Guangxi Vocational & Technical Institute of Industry, 37 Xiuling Road, Nanning, Guangxi 530004, P.R. China *Corresponding author: Fax: +86 771 3231590; Tel: +86 771 3237329; E-mail: liuan6126@126.com

Corresponding Author(s) : Xinquan Liang1

Asian Journal of Chemistry, Vol. 26 No. 23 (2014)

Abstract

A reversed phase high-performance liquid chromatography method was used in simultaneous determination of 6 monomer phenols (e.g., gallic acid, catechin, chlorogenic acid, caffeic acid, epicatechin and ferulic acid) in cane juice. A variable wavelength UV detector and a C18 column (250 mm × 4.6 mm 5 μm) were used. The mixture of methanol, acetic acid and water were used as the mobile phase in a gradient mode. Five monomer phenols (gallic acid, chlorogenic acid, caffeic acid, epicatechin and ferulic acid) were detected in cane juice with an average recovery ratio of 81.6-93.7 % and the relative standard deviations (RSD) were less than 5 %. This method is a convenient, rapid and accurate way for simultaneous determination of monomer phenols in cane juice.

Keywords

RP-HPLC Cane juice Phenols.
Liang1, X., Liang1, X., He2, H., Liu1, J., & Lu1, D. (2014). Determination of Monomer Phenols in Cane Juice by Reversed-Phase High-Performance Liquid Chromatography with UV Detection. Asian Journal of Chemistry, 26(23), 8123–8126. https://doi.org/10.14233/ajchem.2014.17596
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