Issue

Vol. 26 No. 23 (2014)

Copyright (c) 2014 Y.J. Zou, C.L. Xiang, L.X. Sun*, F. Xu, H.Y. Zhou

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Ultrasensitive Prostate Specific Antigen Immunosensor Based on Gold Nanoparticles Functionalized Polypyrrole@Carbon Nanotubes

https://doi.org/10.14233/ajchem.2014.16896
Y.J. Zou
Guangxi Key Laboratory of Information Materials, Guilin University of Electronic Technology, 1 Jinji Road, Guilin 541004, P.R. China *Corresponding author: Fax: +86 773 2191903; Tel: +86 773 2303763; E-mail: sunlx@guet.edu.cn
C.L. Xiang
Guangxi Key Laboratory of Information Materials, Guilin University of Electronic Technology, 1 Jinji Road, Guilin 541004, P.R. China *Corresponding author: Fax: +86 773 2191903; Tel: +86 773 2303763; E-mail: sunlx@guet.edu.cn
L.X. Sun*
Guangxi Key Laboratory of Information Materials, Guilin University of Electronic Technology, 1 Jinji Road, Guilin 541004, P.R. China *Corresponding author: Fax: +86 773 2191903; Tel: +86 773 2303763; E-mail: sunlx@guet.edu.cn
F. Xu
Guangxi Key Laboratory of Information Materials, Guilin University of Electronic Technology, 1 Jinji Road, Guilin 541004, P.R. China *Corresponding author: Fax: +86 773 2191903; Tel: +86 773 2303763; E-mail: sunlx@guet.edu.cn
H.Y. Zhou
Guangxi Key Laboratory of Information Materials, Guilin University of Electronic Technology, 1 Jinji Road, Guilin 541004, P.R. China *Corresponding author: Fax: +86 773 2191903; Tel: +86 773 2303763; E-mail: sunlx@guet.edu.cn

Corresponding Author(s) : Y.J. Zou

Asian Journal of Chemistry, Vol. 26 No. 23 (2014)

Abstract

In this paper, an ultrasensitive electrochemical immunosensor for the detection of prostate specific antigen (PSA) based on gold nanoparticles functionalized polypyrrole@carbon nanotubes nanocomposite was reported. Pyrrole monomer was polymerized on MWNTs substrate in acid solution. Negatively charged gold nanoparticles (AuNPs) were assembled on the PPy@MWNTs by electrostatic interactions. With the primary antibody (Ab1) immobilized on the AuNPs/PPy@MWNTs nanocomposite, a sandwich electrochemical immunosensor for the detection of prostate specific antigen was constructed. The electrochemical signal was amplified substantially by the nanocomposite. A wide linear range from 0.002 to 20 ng mL-1 with a detection limit of 0.001 ng mL-1 (the ratio of signal to noise, S/N = 3:1) was obtained, which is comparable or even better than most of the prostate specific antigen immunosensor. Furthermore, the prepared immunosensor showed excellent selectivity and reproducibility.

Keywords

Gold nanoparticles MWNT Polypyrrole Prostate specific antigen Electrochemical immunosensor.
Zou, Y., Xiang, C., Sun*, L., Xu, F., & Zhou, H. (2014). Ultrasensitive Prostate Specific Antigen Immunosensor Based on Gold Nanoparticles Functionalized Polypyrrole@Carbon Nanotubes. Asian Journal of Chemistry, 26(23), 8002–8006. https://doi.org/10.14233/ajchem.2014.16896
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. W. Chen, Z. Lu and C.M. Li, Anal. Chem., 80, 8485 (2008).
  2. X. Yang, L. Li and Y. Zhao, Synth. Met., 160, 1822 (2010).
  3. K.V. Singh, D.K. Bhura, G. Nandamuri, A.M. Whited, D. Evans, J. King and R. Solanki, Langmuir, 27, 13931 (2011).
  4. H. Su, R. Yuan, Y. Chai and Y. Zhuo, Biosens. Bioelectron., 33, 288 (2012).
  5. W. Sun, K. Jiao, S. Zhang, C. Zhang and Z. Zhang, Anal. Chim. Acta, 434, 43 (2001).
  6. Y. Wan, P. Qi, D. Zhang, J. Wu and Y. Wang, Biosens. Bioelectron., 33, 69 (2012).
  7. C. Chen, D. Liu, Z. Wu, Q. Luo, G.L. Shen and R.Q. Yu, Electrochem. Commun., 11, 1869 (2009).
  8. Q. Wei, Z. Xiang, J. He, G. Wang, H. Li, Z. Qian and M. Yang, Biosens. Bioelectron., 26, 627 (2010).
  9. J. Nie, Y. Zhang, H. Wang, S. Wang and G. Shen, Biosens. Bioelectron., 33, 23 (2012).
  10. J. Wang, G. Liu and M.R. Jan, J. Am. Chem. Soc., 126, 3010 (2004).
  11. L. Qu, S. Xia, C. Bian, J. Sun and J. Han, Biosens. Bioelectron., 24, 3419 (2009).
  12. Y.G. Zhou, N.V. Rees and R.G. Compton, Chem. Commun., 48, 2510 (2012).
  13. H. Liu, X. Wu, X. Zhang, C. Burda and J.J. Zhu, J. Phys. Chem. C, 116, 2548 (2012).
  14. C. Xiang, Y. Zou, L.X. Sun and F. Xu, Sens. Actuator B, 136, 158 (2009).
  15. J.J. Lu, S. Liu, S. Ge, M. Yan, J. Yu and X. Hu, Biosens. Bioelectron., 33, 29 (2012).
  16. R. Zanella, E.V. Basiuk, P. Santiago, V.A. Basiuk, E. Mireles, I. Puente-Lee and J.M. Saniger, J. Phys. Chem. B, 109, 16290 (2005).
  17. B. Kim and W.M. Sigmund, Langmuir, 20, 8239 (2004).
  18. K.C. Grabar, K.J. Allison, B.E. Baker, R.M. Bright, K.R. Brown, R.G. Freenman, A.P. Fox, C.D. Keating, M.D. Musick and M.J. Natan, Langmuir, 122, 353 (1996).
  19. G.D. Liu, Y.Y. Lin, J. Wang, H. Wu, C.M. Wai and Y.H. Lin, Anal. Chem., 79, 7644 (2007).
  20. K. Mao, D. Wu, Y. Li, H. Ma, Z. Ni, H. Yu, C. Luo, Q. Wei and B. Du, Anal. Biochem., 422, 22 (2012).
  21. Q. Wei, Z. Xiang, J. He, G. Wang, H. Li, Z. Qian and M. Yang, Biosens. Bioelectron., 26, 627 (2010).
  22. H. Li, Q. Wei, G. Wang, M. Yang, F. Qu and Z. Qian, Biosens. Bioelectron., 26, 3044 (2011).
Read More
Author biographies is not available.
Download
Statistic
Read Counter : 0