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Vol. 26 No. 23 (2014)

Copyright (c) 2014 Xiaoshuang Chen1, Gaofeng Li2, Xujia Hu1

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Simultaneous Quantification of Berberine Hydrochloride and Fibrauretine Tablets in Rat Urine, Feces, Bile and Plasma by HPLC/DAD

https://doi.org/10.14233/ajchem.2014.16876
Xiaoshuang Chen1
1Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, P.R. China 2The Third Affiliated Hospital of Kunming Medical University, Kunming, P.R. China *Corresponding author: Fax: +86 871 5920570; Tel: +86 871 5920672; E-mail: huxjia@gmail.com; 526146702@qq.com
Gaofeng Li2
1Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, P.R. China 2The Third Affiliated Hospital of Kunming Medical University, Kunming, P.R. China *Corresponding author: Fax: +86 871 5920570; Tel: +86 871 5920672; E-mail: huxjia@gmail.com; 526146702@qq.com
Xujia Hu1
1Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, P.R. China 2The Third Affiliated Hospital of Kunming Medical University, Kunming, P.R. China *Corresponding author: Fax: +86 871 5920570; Tel: +86 871 5920672; E-mail: huxjia@gmail.com; 526146702@qq.com

Corresponding Author(s) : Xiaoshuang Chen1

Asian Journal of Chemistry, Vol. 26 No. 23 (2014)

Abstract

Isoquinoline alkaloids i.e., berberine and palmatine are bioactive components of berberine hydrochloride tablets and fibrauretine tablets, respectively. For pharmacological and pharmacokinetics purposes, a simple, rapid and sensitive HPLC/DAD method was employed to measure them in rat urine, feces, bile and plasma. After simple deproteinization by acetonitrile, the ethyl acetate/methanol extract was directly analyzed by HPLC/DAD using a kromasil C18 column (200 × 4.6 mm 5 μm) with UV detection at 265 nm, with the mobile phase comprising 0.2 % phosphoric acid buffer-acetonitrile (74:26, v/v), at a flow rate of 1 mL min-1. Calibration curves of palmatine hydrochloride and berberine hydrochloride in rat urine, feces, bile and plasma were linear over the concentration range of 0.3-75 ng/mL. After oral administration of 30 mg/kg berberine hydrochloride tablets and fibrauretine tablets, cumulative excretion amount of palmatine hydrochloride and berberine hydrochloride in rat plasma were 10.14 ± 0.71 and 0.13 ± 0.06 ng at 24 h, respectively. The cumulative amounts of palmatine hydrochloride and berberine hydrochloride in rat urine reached 1.18 ± 0.28 and 16.32 ± 0.43 ng at 36 h after dosing, respectively. The results showed for their pharmacological differences study. Validation revealed that the method was specific, accurate and precise. The fully validated method was applied to the excretion study of berberine hydrochloride and palmatine hydrochloride in rats.

Keywords

HPLC/DAD Berberine hydrochloride Fibrauretine Rat urine Feces Bile and plasma Pharmacological profile study.
Chen1, X., Li2, G., & Hu1, X. (2014). Simultaneous Quantification of Berberine Hydrochloride and Fibrauretine Tablets in Rat Urine, Feces, Bile and Plasma by HPLC/DAD. Asian Journal of Chemistry, 26(23), 7994–7998. https://doi.org/10.14233/ajchem.2014.16876
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