Detection of Thrombin Using Label-Free Electrochemical Aptamer Sensor Based on Bimetallic Pd-Au Nanoparticles

G.Y. Shen; S.B. Zhang; X. Hu; A.G. Xiao

doi:10.14233/ajchem.2014.17450

Issue

Vol. 26 No. 22 (2014): Vol 26 Issue 22

Issue Published : November 6, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Detection of Thrombin Using Label-Free Electrochemical Aptamer Sensor Based on Bimetallic Pd-Au Nanoparticles

https://doi.org/10.14233/ajchem.2014.17450

G.Y. Shen

College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, P.R. China

S.B. Zhang

College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, P.R. China

X. Hu

College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, P.R. China

A.G. Xiao

College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, P.R. China

Corresponding Author(s) : G.Y. Shen

sgyrab@163.com

Asian Journal of Chemistry, Vol. 26 No. 22 (2014): Vol 26 Issue 22
Article Published : November 6, 2014

Abstract

In this paper, we reported a novel label-free electrochemical aptamer sensor based on the catalytic activity of Pd-Au nanoparticles towards H₂O₂ for the thrombin detection. In the sensing strategy, the Au electrode was first modified with Pd-Au-chitosan film and then the aptamer was immobilized on the surface of Pd-Au nanoparticles. In the presence of thrombin, the aptamer on the surface of the modified electrode would capture thrombin on the electrode interface, which makes a barrier to electro-transfer, resulting in the decreased cyclic voltammetry signals. Using this strategy, thrombin was detected within the range from 0.1 to 25 nM with a detection limit of 0.06 nM (S/N = 3). The aptamer sensor had a good specificity, stability and reproducibility. This simple strategy may pave a way to fabricate a simple aptamer sensors in clinical application.

Keywords

Pd-Au nanoparticles Aptamer sensor Label-free Thrombin

Shen, G., Zhang, S., Hu, X., & Xiao, A. (2014). Detection of Thrombin Using Label-Free Electrochemical Aptamer Sensor Based on Bimetallic Pd-Au Nanoparticles. Asian Journal of Chemistry, 26(22), 7647–7650. https://doi.org/10.14233/ajchem.2014.17450

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S.R. Coughlin, Nature, 407, 258 (2000); doi:10.1038/35025229.

M.L. Nierodzik and S. Karpatkin, Cancer Cell, 10, 355 (2006); doi:10.1016/j.ccr.2006.10.002.

S. Jayasena, Clin. Chem., 45, 1628 (1999).

S. Clark and V. Remcho, Electrophoresis, 23, 1335 (2002); doi:10.1002/1522-2683(200205)23:9<1335::AID-ELPS1335>3.0.CO;2-E.

R. Nutiu and Y. Li, Chem. Eur. J., 10, 1868 (2004); doi:10.1002/chem.200305470.

C.K. O’Sullivan, Anal. Bioanal. Chem., 372, 44 (2002); doi:10.1007/s00216-001-1189-3.

M. Mir, M. Vreeke and I. Katakis, Electrochem. Commun., 8, 505 (2006); doi:10.1016/j.elecom.2005.12.022.

F. Le Floch, H.A. Ho and M. Leclerc, Anal. Chem., 78, 4727 (2006); doi:10.1021/ac0521955.

A.-E. Radi, J.L. Acero Sánchez, E. Baldrich and C.K. O’Sullivan, Anal. Chem., 77, 6320 (2005); doi:10.1021/ac0505775.

S. Centi, S. Tombelli, M. Minunni and M. Mascini, Anal. Chem., 79, 1466 (2007); doi:10.1021/ac061879p.

K. Ikebukuro, C. Kiyohara and K. Sode, Biosens. Bioelectron., 20, 2168 (2005); doi:10.1016/j.bios.2004.09.002.

H. Yang, J. Ji, Y. Liu, J. Kong and B. Liu, Electrochem. Commun., 11, 38 (2009); doi:10.1016/j.elecom.2008.10.024.

G.D. Liu, T.M.H. Lee and J.S. Wang, J. Am. Chem. Soc., 127, 38 (2005); doi:10.1021/ja043780a.

Y. Han, J. Zheng and S. Dong, Electrochim. Acta, 90, 35 (2013); doi:10.1016/j.electacta.2012.11.117.

J.M. You, D. Kim, S.K. Kim, M.S. Kim, H.S. Han and S. Jeon, Sens. Actuators B, 178, 450 (2013); doi:10.1016/j.snb.2013.01.006.

F. Meng, X. Yan, J. Liu, J. Gu and Z. Zou, Electrochim. Acta, 56, 4657 (2011); doi:10.1016/j.electacta.2011.02.105.

H. Lu, S. Yu, Y. Fan, C. Yang and D. Xu, Colloids Surf. B, 101, 106 (2013); doi:10.1016/j.colsurfb.2012.05.033.

S. Alayoglu, A.U. Nilekar, M. Mavrikakis and B. Eichhorn, Nat. Mater., 7, 333 (2008); doi:10.1038/nmat2156.

Q. Wei, Y.F. Zhao, B. Du, D. Wu, Y.Y. Cai, K.X. Mao, H. Li and C.X. Xu, Adv. Funct. Mater., 21, 4193 (2011); doi:10.1002/adfm.201100773.

D.S. Wang and Y.D. Li, Adv. Mater., 23, 1044 (2011); doi:10.1002/adma.201003695.

D. Kim, Y.W. Lee, S.B. Lee and S.W. Han, Angew. Chem. Int. Ed., 51, 159 (2012); doi:10.1002/anie.201106899.

M. Tao, X. Li, Z. Wu, M. Wang, M. Hua and Y. Yang, Clin. Chim. Acta, 412, 550 (2011); doi:10.1016/j.cca.2010.12.004.

L. Zhao, S. Li, J. He, G. Tian, Q. Wei and H. Li, Biosens. Bioelectron., 49, 222 (2013); doi:10.1016/j.bios.2013.05.016.

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X. Ren and P.G. Pickup, J. Electroanal. Chem., 420, 251 (1997); doi:10.1016/S0022-0728(96)04784-5.

H.Y. Liu, R. Malhotra, M.W. Peczuh and J.F. Rusling, Anal. Chem., 82, 5865 (2010); doi:10.1021/ac101110q.

H. Xu, X. Mao, Q. Zeng, S. Wang, A. Kawde and G. Liu, Anal. Chem., 81, 669 (2009); doi:10.1021/ac8020592.

C.F. Ding, Y. Ge and J.M. Lin, Biosens. Bioelectron., 25, 1290 (2010); doi:10.1016/j.bios.2009.10.017.

Issue

Vol. 26 No. 22 (2014): Vol 26 Issue 22

Detection of Thrombin Using Label-Free Electrochemical Aptamer Sensor Based on Bimetallic Pd-Au Nanoparticles

Corresponding Author(s) : G.Y. Shen

Abstract

Keywords

Full Article

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