Copyright (c) 2014 AJC
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Estimation of Germanicol by Validated HPTLC Densitometric Method in Leaves of Different Species of Genus Ficus
Corresponding Author(s) : P. Alam
Asian Journal of Chemistry,
Vol. 26 No. 22 (2014): Vol 26 Issue 22
Abstract
A simple and sensitive HPTLC method was developed for estimation of germanicol in the leaves of five different species of genus Ficus (Ficus carica, Ficus nitida, Ficus ingens, Ficus palmata and Ficus vasta) grown in Kingdom of Saudi Arabia. The chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with solvents hexane: chloroform (55:45, v/v) as mobile phase. After the development of HPTLC plate it was derivatized with p-anisaldehyde reagent to give well resolved and compact spot of germanicol. The developed method was found to give a compact spot for germanicol (Rf = 0.39 ± 0.01) at 525 nm. Germanicol was found to be present in four species i.e., F. carica (0.21%, w/w), F. nitida (0.22%, w/w), F. palmata (1.06%, w/w) and F. vasta (0.27%, w/w) while it was absent in the F. final. The proposed method can be used in study of degradation kinetics of germenicol and its determination in plasma.
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J. Hutchinson, J.M. Dalziel and R.W.J. Keay, Flora of West Tropical Africa, Crown Agents, London, edn. 2 (1958).
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C. Chunyan, S. Bo, L. Ping, L. Jingmei and Y. Ito, J. Liq. Chromatogr. Rel. Technol., 32, 136 (2008); doi:10.1080/10826070802548747.
M.S. Chang, Y.C. Yang, Y.C. Kuo, Y.H. Kuo, C. Chang, C.M. Chen and T.H. Lee, J. Nat. Prod., 68, 11 (2005); doi:10.1021/np0401056.
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S.C. Mandal, T.K. Maity, J. Das, B.P. Saha and M. Pal, Nat. Prod. Sci., 4, 174 (1998).
C.V. Rao, A.R. Verma, M. Vijayakumar and S. Rastogi, J. Ethnopharmacol., 115, 323 (2008); doi:10.1016/j.jep.2007.09.019.
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G. Topcua, A. Ertas, U. Kolakb, M. Ozturk and A. Ulubelenb, ARKIVOC, 195 (2007).
L.B. Rojas, M. Grignon-Dubois, B. Rezzonico and A. Usubillaga, Nat. Comput., 40, 565 (2004); doi:10.1007/s10600-005-0037-7.
S. Mishra and S. Sahoo, World J. Pharm. Pharm. Sci., 2, 1781 (2013).
C.Y. Ragasa, D.L. Espineli and C.C. Shen, Nat. Prod. Res., 26, 1869 (2012); doi:10.1080/14786419.2011.619187.
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D.K. Kim, J.P. Lim, J.W. Kim, H.W. Park and J.S. Eun, Arch. Pharm. Res., 28, 39 (2005); doi:10.1007/BF02975133.
A.C. Casabuono and Pomilio, Lipids, 32, 205 (1997); doi:10.1007/s11745-997-0026-z.
International Conference on Harmonization (ICH) of Technical Requirements for Registration of Pharmaceuticals for Human Use, Harmonised Triplicate Guideline on Validation of Analytical Procedures: Methodology, Recommended for Adoption at Step 4 of the ICH Process on November 1996 by the ICH Steering Committee, IFPMA, Geneva.
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M.F. Alajmi, P. Alam and F. Shakeel, J. Planar Chromatogr., 26, 475 (2013); doi:10.1556/JPC.26.2013.6.3.
G. Biringanine, M.T. Chiarelli, M. Faes and P. Duez, Talanta, 69, 418 (2006); doi:10.1016/j.talanta.2005.10.007.
L. Berkovich, I. Ron, G. Earon, S. Abu-Ghanem, A. Rimmon and S. Lev-Ari, Harefuah, 151, 629 (2012).
E.P. Lansky, H.M. Paavilainen, A.D. Pawlus and R.A. Newman, J. Ethnopharmacol., 119, 195 (2008); doi:10.1016/j.jep.2008.06.025.