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Vol. 26 No. 21 (2014): Vol 26 Issue 21

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Synthesis of Thiosemicarbazones as Substrates for Xanthine Oxidase Enzyme Activity

https://doi.org/10.14233/ajchem.2014.17768
S. Beyaztas
Balikesir University, Science and Art Faculty, Department of Chemistry/Biochemistry Section, Balikesir, Turkey
E. Findik
Gaziosmanpasa University, Science and Art Faculty, Department of Chemistry/Organic Chemistry Section, Tokat, Turkey
M. Ceylan
Gaziosmanpasa University, Science and Art Faculty, Department of Chemistry/Organic Chemistry Section, Tokat, Turkey
O. Arslan
Balikesir University, Science and Art Faculty, Department of Chemistry/Biochemistry Section, Balikesir, Turkey

Corresponding Author(s) : S. Beyaztas

beyaztas@balikesir.edu.tr

Asian Journal of Chemistry, Vol. 26 No. 21 (2014): Vol 26 Issue 21

Abstract

Thiosemicarbazones were synthesized from 7-arylbicyclo[3.2.0]hept-2-en-6-ones (1a-d) and thiosemicarbazide. The compounds were purified on a silica gel column chromatography. The thiosemicarbazone compounds were tested in vitro effect on xanthine oxidase (XO) purified from bovine milk. These compounds exhibited activator effects on xanthine oxidase enzyme activity at low concentration. We examined KM and Vmax values for thiosemicarbazone derivatives at different pH values. The derivatives showed better values of KM, Vmax and Vmax/KM than xanthine. Particularly, (Z)-1-((1R,5S,E)-7-(4-methylbenzylidene)bicyclo[3.2.0]hept-2-en-6-ylidene) thiosemicarbazide (4Mtc) was the most suitable substrate, due to the lowest KM and the highest Vmax/KM values. KM and Vmax/KM values were 1 ×10-4 M and 1.11 × 106 min-1, respectively. We proposed here a novel substrate for xanthine oxidase which can be used to assess the activity of this enzyme.

Keywords

Xanthine oxidase in vitro Effect Kinetic parameters Substrate Thiosemicarbazone derivatives
Beyaztas, S., Findik, E., Ceylan, M., & Arslan, O. (2014). Synthesis of Thiosemicarbazones as Substrates for Xanthine Oxidase Enzyme Activity. Asian Journal of Chemistry, 26(21), 7467–7471. https://doi.org/10.14233/ajchem.2014.17768
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