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Simultaneous Determination of Total Flavonoids and Organic Acids in Chrysanthemum by UV-Visible Spectrophotometry
Corresponding Author(s) : Jing Tang
Asian Journal of Chemistry,
Vol. 26 No. 19 (2014): Vol 26 Issue 19
Abstract
A rapid method for simultaneous determination of total flavonoids and total organic acids in different kinds of chrysanthemum was developed. The content of total flavonoids was determined at 270 nm by UV method using luteolin as standard and that of total phenols was done at 510 nm using the color reaction with Al(NO3)3 using the colored luteolin as standard. The optimum extraction conditions of flavonoids from chrysanthemum were investigated by using orthogonal design. The content of total organic acids is the value for the differences between the content of total phenols and that of total flavonoids. Due to the reports of the methods of determining the content of total organic acids in chrysanthemum is few. This method can be relatively simple measure the content of total organic acids in chrysanthemum.
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References
J.W. Jian and Q.X. Xiao, Phytomedicine Effective Component Book, People's Hygiene Press, Beijing, p. 351 (1986).
T. Wu, Y. Guan and J. Ye, Food Chem., 100, 1573 (2007); doi:10.1016/j.foodchem.2005.12.042.
D. Prakash, B.N. Singh and G. Upadhyay, Food Chem., 102, 1389 (2007); doi:10.1016/j.foodchem.2006.06.063.
J. Wang, Y.M. Zhao, C.Y. Guo, S.M. Zhang, C.L. Liu, D.S. Zhang and X.M. Bai, Phcog. Mag., 8, 166 (2012); doi:10.4103/0973-1296.96581.
Y. Chen, J. Wang and D. Wan, Phcog Mag, 6, 259 (2010); doi:10.4103/0973-1296.71784.
S.P. Wang and K.J. Huang, J. Chromatogr. A, 1032, 273 (2004); doi:10.1016/j.chroma.2003.11.099.
G.S. Kartashova and L.V. Sudos, Pharm. Chem. J., 31, 488 (1997); doi:10.1007/BF02464310.
D. Heimler, A. Pieroni, M. Tattini and A. Cimato, Chromatographia, 33, 369 (1992); doi:10.1007/BF02275920.
D. Marinova, F. Ribarova and M. Atanassova, J. Univ. Chem. Technol. Metallurgy, 40, 255 (2005).
L.A. Soares, V.L. Bassani, G.G. Ortega and P.R. Petrovick, Lat. Am. J. Pharm., 22, 203 (2003).