Issue

Vol. 26 No. 16 (2014): Vol 26 Issue 16

Copyright (c) 2014 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Comparison of Natural and Synthetic a-Tocopherol in Rat Plasma by HPLC and Euryale ferox Seed as a Source of Natural a-Tocopherol

https://doi.org/10.14233/ajchem.2014.17197
Xiaoxiao Wang
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, P.R. China
Yongxian Cheng
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P.R. China
Xiaoshuang Chen
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, P.R. China
Jinhua Liao
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, P.R. China
Juan Yang
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, P.R. China
Xujia Hu
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, P.R. China

Corresponding Author(s) : Xujia Hu

huxjia@gmail.com

Asian Journal of Chemistry, Vol. 26 No. 16 (2014): Vol 26 Issue 16

Abstract

A validated HPLC with UV method was developed to evaluate the difference between natural (RRR) a-tocopherol and synthetic (all-rac) a-tocopherol in rat plasma by an intragastric administrated does of a-tocopherol acetate. The direct extraction of analytes in two steps with methanol/isopropanol (8:2, v/v) and n-hexane. This method employed a Kromasil C18 column and methanol/water (98:2, v/v) as mobile phase, at a flow rate of 1 mL min-1. The limits of detection (LOD) were 0.12 μg mL-1 for DL-a-tocopherol acetate and 0.1 μg mL-1 for DL-a-tocopherol, respectively. The recoveries of the method were near 95 %. Intra-assay and inter-assay precision were, respectively 2.3-5.5 % and 2.1-7.9 %. The method was employed to evaluate natural (RRR) and synthetic (all rac) a-tocopherol in rat plasma. Meanwhile, the a-tocopherol content in Euryale ferox seed was measured. The results obtained from this study demonstrated that natural a-tocopherol was less accumulated than synthetic a-tocopherol and safe for long term use. After measurement, Euryale ferox seed is a potentially rich source of natural tocopherols and could be used as antioxidants in food for nutraceutical purpose.

Keywords

a-tocopherol Vitamin E HPLC Rat plasma Euryale ferox seed
Wang, X., Cheng, Y., Chen, X., Liao, J., Yang, J., & Hu, X. (2014). Comparison of Natural and Synthetic a-Tocopherol in Rat Plasma by HPLC and Euryale ferox Seed as a Source of Natural a-Tocopherol. Asian Journal of Chemistry, 26(16), 5279–5282. https://doi.org/10.14233/ajchem.2014.17197
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. H. Esterbauer, D.R. Mieber, G. Striegl and G. Waeg, Am. J. Clin. Nutr., 53, 314S (1991).
  2. H. Weiser and M. Vecchi, Int. J. Vitam. Nutr. Res., 51, 100 (1981).
  3. T. Netscher, Chimia, 50, 563 (1996).
  4. T. Netscher, Synthesis and Production of Vitamin E, Sheffield, UK, p. 26 (1999).
  5. L. Shargel and A.B.C. Yu, Applied Biopharmaceutics and Pharmacokinetics, Appleton-Lange, Stamford CT, p.34(1999).
  6. S. Kijima and M. Mino, Chemistry of Vitamin E, Japan Sci. Soc., Tokyo, p. 45 (1993).
  7. G. Riss, A.W. Kormann, E. Glinz, W. Walther and U.B. Ranalder, Methods Enzymol., 234, 302 (1994); doi:10.1016/0076-6879(94)34097-8.
  8. M.G. Traber, A. Elsner and R. Brigelius-Flohé, FEBS Lett., 437, 145 (1998); doi:10.1016/S0014-5793(98)01210-1.
  9. W.B. Graham, G.T. Maret and V.A. Robert, Am. J. Clin. Nutr., 67, 669 (1998).
  10. H. Weiser, M. Vecchi and M. Schlachter, Int. J. Vitam. Nutr. Res., 56, 45 (1986).
  11. E. Wooltorton, Health Drug Alerts, 169, 47 (2003).
  12. D.C. Schwenke, J. Nutr. Biochem., 13, 2 (2002); doi:10.1016/S0955-2863(01)00207-8.
  13. China Pharmacopoeia Committee, Pharmacopoeia of People’s Republic of China, Beijing (2010).
  14. M. Thompson, S.L.R. Ellison and R. Wood, Pure Appl. Chem., 74, 835 (2002); doi:10.1351/pac200274050835.
  15. D.H. Emmert and J.T. Kirchner, Arch. Fam. Med., 8, 537 (1999); doi:10.1001/archfami.8.6.537.
  16. A.P. Jain, A. Mohan, O.P. Gupta, U.N. Jajoo, S.P. Kalantri and L.M. Srivastava, Ind. J. Clin. Biol., 13, 119 (1998); doi:10.1007/BF02867873.
  17. J. Fuchs, Free Radic. Biol. Med., 25, 848 (1998); doi:10.1016/S0891-5849(98)00161-0.
  18. I.M. Ernst, K. Pallauf, J.K. Bendall, L. Paulsen, S. Nikolai, P. Huebbe, T. Roeder and G. Rimbach, Ageing Res. Rev., 12, 365 (2013); doi:10.1016/j.arr.2012.10.002.
  19. D.E. Kaempf-Rotzoll, M.G. Traber and H. Arai, Curr. Opin. Lipidol., 14, 249 (2003); doi:10.1097/00041433-200306000-00004.
  20. D.H. Blatt, S.W. Leonard and M.G. Traber, Nutrition, 17, 799 (2001); doi:10.1016/S0899-9007(01)00637-2.
  21. M.G. Traber and H. Arai, Annu. Rev. Nutr., 19, 343 (1999); doi:10.1146/annurev.nutr.19.1.343.
  22. Food and Nutrition Board, Institute of Medicine Dietary Reference Intakes for Vitamin C, Vitamin E, Selenium, and Carotenoids. National Academy Press, Washington, DC (2000).
  23. G.W. Burton, M.G. Traber, R.V. Acuff, D.N. Walters, H.J. Kayden, L. Hughes and K.U. Ingold, Am. J. Clin. Nutr., 67, 669 (1998).
  24. M.G. Traber, D. Rader, R.V. Acuff, H.B. Brewer Jr. and H.J. Kayden, Atherosclerosis, 108, 27 (1994); doi:10.1016/0021-9150(94)90035-3.
  25. C.-W. Song, S.-M. Wang, L.-L. Zhou, F.-F. Hou, K.-J. Wang, Q.-B. Han, N. Li and Y.-X. Cheng, J. Agric. Food Chem., 59, 1199 (2011); doi:10.1021/jf1041933.
  26. EC, Council Directive of September 1990 on Nutrition Labelling for Foodstuffs (90/496/EEC); The Council of the European Communities, Brussels (1990).
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 1 Download : 0