Rapid Determination of Vitamin B2 in Foods by HPLC with in Capillary Optical Fiber Laser-Induced Fluorescence Detection Technique

Y. Tang; J. Zhang; L.M. Teng; Y.Y. He; D. Xiao

doi:10.14233/ajchem.2014.16278

Issue

Vol. 26 No. 16 (2014): Vol 26 Issue 16

Issue Published : July 28, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Rapid Determination of Vitamin B2 in Foods by HPLC with in Capillary Optical Fiber Laser-Induced Fluorescence Detection Technique

https://doi.org/10.14233/ajchem.2014.16278

Y. Tang

Chongqing Key Laboratory of Environmental Materials & Remediation Technologies, Chongqing University of Arts and Sciences, Chongqing 402160, P.R. China

J. Zhang

Chongqing Key Laboratory of Environmental Materials & Remediation Technologies, Chongqing University of Arts and Sciences, Chongqing 402160, P.R. China

L.M. Teng

Chongqing Key Laboratory of Environmental Materials & Remediation Technologies, Chongqing University of Arts and Sciences, Chongqing 402160, P.R. China

Y.Y. He

2College of Chemistry and College of Chemical Engineering, Sichuan University, Chengdu 610064, P.R. China

D. Xiao

2College of Chemistry and College of Chemical Engineering, Sichuan University, Chengdu 610064, P.R. China

Corresponding Author(s) : J. Zhang

hxxzj2282@163.com

Asian Journal of Chemistry, Vol. 26 No. 16 (2014): Vol 26 Issue 16
Article Published : August 4, 2014

Abstract

In this paper, HPLC with in-capillary optical fiber laser-induced fluorescence (HPLC-ICOF-laser induced fluorescence) detection method has been developed for determination of vitamin B2 (riboflavin). The HPLC separation of vitamin B2 in foods and the ICOF-laser induced fluorescence detection system are studied and optimized. Optimum separation conditions are: mobile phase consisted of methanol/distilled water was 6:4(v/v)in Zorbax Eclipse XDB-C18 column, flow rate was constant at 1 mL min^-1, the injection volume was 20 μL. The ICOF-laser induced fluorescence detection system comprises a 530 μm capillary and a 380 μm optical fiber. This method is not only relatively simple chromatographic conditions but also high sensitivity and good precision. It reduces the limit of detection to 0.2 nM and expends the linear range to 0.35-177.14 nM. The correlation coefficient in this range was 0.999. This method can be used in the analysis of trace riboflavin in the food.

Keywords

Column liquid chromatography In-capillary optical fiber laser-induced Fluorescence detection Vitamin B2 (riboflavin)

Tang, Y., Zhang, J., Teng, L., He, Y., & Xiao, D. (2014). Rapid Determination of Vitamin B2 in Foods by HPLC with in Capillary Optical Fiber Laser-Induced Fluorescence Detection Technique. Asian Journal of Chemistry, 26(16), 4968–4970. https://doi.org/10.14233/ajchem.2014.16278

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References

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References

X. Tang, D.A. Cronin and N.P. Brunton, J. Food Compos. Anal., 19, 831 (2006); doi:10.1016/j.jfca.2005.12.013.

P. Dubbert, A. King, S. Rapp, D. Brief, J. Martin and M. Lake, J. Behav. Med., 8, 287 (1985); doi:10.1007/BF00870315.

B. Switzer, A. Star, J. Atwood, C. Ritenbaugh, R. Travis and H. Wu, Cancer Epidemiol., 6, 439 (1997).

A.M. Bishop, C. Fernandez, R.D. Whitehead Jr., P. Morales-A, D.B. Barr, L.C. Wilder and S.E. Baker, J. Chromatogr. B, 879, 1823 (2011); doi:10.1016/j.jchromb.2011.04.032.

B.J. Petteys and E.L. Frank, Clin. Chim. Acta, 412, 38 (2011); doi:10.1016/j.cca.2010.08.037.

P.F. Chatzimichalakis, V.F. Samanidou, R. Verpoorte and I.N. Papadoyannis, J. Sep. Sci., 27, 1181 (2004); doi:10.1002/jssc.200401858.

V.J. Gatautis and H.K. Natio, Clin. Chem., 27, 1672 (1981).

W.E. Lambert, P.M. Cammaert and A.P.D. Leenheer, Clin. Chem., 31, 1371 (1985).

M. Chen, D.M. Andrenyak, D.E. Moody and R.L. Foltz, J. Chromatogr. B, 820, 147 (2005); doi:10.1016/j.jchromb.2005.03.018.

S.M. Mandal, M. Mandal, A.K. Ghosh and S. Dey, Anal. Chim. Acta, 640, 110 (2009); doi:10.1016/j.aca.2009.03.009.

P. Britz-McKibbin, M.J. Markuszewski, T. Iyanagi, K. Matsuda, T. Nishioka and S. Terabe, Anal. Biochem., 313, 89 (2003); doi:10.1016/S0003-2697(02)00510-9.

L. Hu, X. Yang, C. Wang, H. Yuan and D. Xiao, J. Chromatogr. B, 856, 245 (2007); doi:10.1016/j.jchromb.2007.06.011.

Y. He, L. Zhao, H. Yuan, Z. Xu, Y. Tang, D. Xiao and M.M.F. Choi, Chromatographia, 74, 541 (2011); doi:10.1007/s10337-011-2112-5.

Issue

Vol. 26 No. 16 (2014): Vol 26 Issue 16

Rapid Determination of Vitamin B2 in Foods by HPLC with in Capillary Optical Fiber Laser-Induced Fluorescence Detection Technique

Corresponding Author(s) : J. Zhang

Abstract

Keywords

Full Article

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