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Degradation Properties of Melamine Wastewater with Streptomyces lincolnensis
Corresponding Author(s) : Yong Xie
Asian Journal of Chemistry,
Vol. 26 No. 16 (2014): Vol 26 Issue 16
Abstract
This study focused on three experiments about utilization of ammonia-nitrogen from melamine wastewater to produce lincomycin by Streptomyces lincolnensis screened from melamine wastewater. All three experiments based on mixing the melamine wastewater and the cultural medium in volume ratio 1:10 in shaking flasks. The results showed that during the first experiment fermentation process, there was no differences in biosynthetic activity of the mycelium between the screened and the control groups. The reductive sugar was rapidly utilized in fermentation anaphase with the concentration of 0.23 mg/L. The starting potency of lincomycin was 2030 IU/mL in the lowest stage and reduced by 70 IU/mL compared to the control. In the second experiment, pH value was 5.96-6.98, which was comparatively lower. The speed of metabolism of amino nitrogen was slow and it was 40 mg/100 mL. The potency of lincomycin was 4550 IU/mL in the lowest stage. The third experiment proved that it was effective to promote mycelia secretion by feeding melamine wastewater medium at mid-late stage of fermentation. The potency of lincomycin was 5040 IU/mL in the end, which was 5.88 % higher than the control group. Therefore, the study of recycling utilization for melamine wastewater in the third experiment is quite necessary and significant.
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References
L. Jian and L. Zhe, Mining Metallurg. Eng., 27, 54 (2007).
L. Jiwu and C. Jingjing, Environ. Prot. Chem. Ind., 28, 8 (2010).
M.A. Castillo, N. Felis, P. Aragón, G. Cuesta and C. Sabater, Int. Biodeter. Biodegrad., 58, 196 (2006); doi:10.1016/j.ibiod.2006.06.020.
L. Xiaobing, Chinese J. Pharm., 30, 246 (1999).
X.-B. Li, G.-R. Zhao, H. Zheng and Y.-J. Yuan, Process Biochem., 42, 662 (2007); doi:10.1016/j.procbio.2006.12.007.
J. Spízek and T. Řezanka, Appl. Microbiol. Biotechnol., 63, 510 (2004); doi:10.1007/s00253-003-1431-3.
Z.S. Liang and C. Ju, Multiscale Process Optimization of Microorganisms, Chemical Industry Press, Beijing (2004).
L. Xiao, H.F. Hang and C. Ju, Ind. Microorg., 38, 7 (2008).
Z. Jin, Y.L. Yang and R.S. Jiao, Chin. J. Biotechnol., 14, 103 (1998).
Z. Jian, Biochemical Test, Shanghai Science and Technology Press, Shanghai, Vol. 24, pp. 50-55 (1980).
Chinese Pharmacopoeia (Part 2), Chemical Industry Press, Beijing, p. 154 (1995).
A.E. Kanouni, I. Zerdani, S. Zaafa, M. Znassni, M. Loutfi and M. Boudouma, World J. Microbiol. Biotechnol., 14, 431 (1998); doi:10.1023/A:1008881731894.
L.M. Liu, Y. Li, G.C. Du and J. Chen, Chin. J. Biotechnol., 19, 745 (2003).