Determination of 20 Amino Acids by HPLC/TOF-MS

Zhen Du; Chenling Fan

doi:10.14233/ajchem.2014.16634

Issue

Vol. 26 No. 16 (2014): Vol 26 Issue 16

Issue Published : July 28, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Determination of 20 Amino Acids by HPLC/TOF-MS

https://doi.org/10.14233/ajchem.2014.16634

Zhen Du

Department of Pharmacy, The First Affiliated Hospital of China Medical University, No. 155 Nanjingbei Road, Heping Dist. 110001, Shenyang Liaoning Province, P.R. China ; School of Pharmacy, China Medical University, No. 92 Beier Road, Heping Dist. 110001, Shenyang Liaoning Province, P.R. China

Chenling Fan

Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, Shenyang 110001, P.R. China

Corresponding Author(s) : Zhen Du

zhong-shan2000@hotmail.com

Asian Journal of Chemistry, Vol. 26 No. 16 (2014): Vol 26 Issue 16
Article Published : August 4, 2014

Abstract

To establish an HPLC/TOF-MS method in the determination of human serum amino acids for the ultra-preclinical diagnosis of different diseases. The human serum samples were added inside the isotope-labeled amino acids as the internal standards, then protein-precipitated with CH₃CN and then dried with nitrogen current. After the synchronous derivation, the Diamond RP-C₁₈ column was used, with 0.1 % formic acid/water-CH₃CN (0.03 % trifluoroacetic acid) as mobile phase. With the separation and the HR scan mode, the 20 kinds of amino acids and their relative isomers could be qualitatively identified and quantitatively determined inside human serum, which could provide the real time monitoring towards the plasma amino acids spectrum, directly and quickly reflecting the metabolism and nutritional status of the patients with malignant diseases.

Keywords

HPLC/TOF-MS Amino acid Simultaneous determination

Du, Z., & Fan, C. (2014). Determination of 20 Amino Acids by HPLC/TOF-MS. Asian Journal of Chemistry, 26(16), 5174–5176. https://doi.org/10.14233/ajchem.2014.16634

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References

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References

D.H. Spackman, W.H. Stein and S. Moore, Anal. Chem., 30, 1190 (1958); doi:10.1021/ac60139a006.

M. Biermann, B. Bardl, S. Vollstädt, J. Linnemann, U. Knüpfer, G. Seidel and U. Horn, Amino Acids, 44, 1225 (2013); doi:10.1007/s00726-013-1459-3.

M. Carnicer, A. ten Pierick, J. van Dam, J.J. Heijnen, J. Albiol, W. van Gulik and P. Ferrer, Microb. Cell Fact., 11, 83 (2012); doi:10.1186/1475-2859-11-83.

V. Sibilia, F. Pagani, E. Mrak, E. Dieci, G. Tulipano and F. Ferrucci, Amino Acids, 43, 1751 (2012); doi:10.1007/s00726-012-1260-8.

J. Bischof, J. Leban, M. Zaja, A. Grothey, B. Radunsky, O. Othersen, S. Strobl, D. Vitt and U. Knippschild, Amino Acids, 43, 1577 (2012); doi:10.1007/s00726-012-1234-x.

S.J. Cooper, G.L. Finney, S.L. Brown, S.K. Nelson, J. Hesselberth, M.J. MacCoss and S. Fields, Genome Res., 20, 1288 (2010); doi:10.1101/gr.105825.110.

F. Ladwig, M. Stahl, U. Ludewig, A.A. Hirner, U.Z. Hammes, R. Stadler, K. Harter and W. Koch, Plant Physiol., 158, 1643 (2012); doi:10.1104/pp.111.192583.

E.T. Parker, H.J. Cleaves, J.P. Dworkin, D.P. Glavin, M. Callahan, A. Aubrey, A. Lazcano and J.L. Bada, Proc. Natl. Acad. Sci. USA, 108, 5526 (2011); doi:10.1073/pnas.1019191108.

S.J. Mihalik, S.F. Michaliszyn, J. de lasHeras, F. Bacha, S. Lee, D.H. Chace, V.R. DeJesus, J. Vockley and S.A. Arslanian, Diabetes Care, 35, 605 (2012); doi:10.2337/DC11-1577.

E. Marco-Urrea, J. Seifert, M. von Bergen and L. Adrian, J. Bacteriol., 194, 4169 (2012); doi:10.1128/JB.00049-12.

R.C.A. Schellekens, F. Stellaard, H.J. Woerdenbag, H.W. Frijlink and J.G.W. Kosterink, Br. J. Clin. Pharmacol., 72, 879 (2011); doi:10.1111/j.1365-2125.2011.04071.x.

B.-M. Iresjö and K. Lundholm, J. Transl. Med., 10, 238 (2012); doi:10.1186/1479-5876-10-238.

Issue

Vol. 26 No. 16 (2014): Vol 26 Issue 16

Determination of 20 Amino Acids by HPLC/TOF-MS

Corresponding Author(s) : Zhen Du

Abstract

Keywords

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