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Simultaneous Determination of Three Active Ingredients in Wubishan Capsule by HPLC
Corresponding Author(s) : Zhen Du
Asian Journal of Chemistry,
Vol. 26 No. 16 (2014): Vol 26 Issue 16
Abstract
To establish an HPLC method for the simultaneous determination of 3 bioactive ingredients in Wubishan capsule. HPLC conditions included Kromasil-C18 column (250 mm × 4.6 mm, 5 μm) and the mobile phase was a mixture of MeOH-CH3CN-H2O (13:6:81). The flow rate was 1.0 mL/min, the column temperature was at 30 °C and the UV detection wavelength was 254 nm. The 3 active ingredients had good linear relationships: guanosine in the range of 0.0915-4.575 μg (R2 = 0.999 3), loganin in the range of 0.116-5.801 μg (R2 = 0.999 0) and schizandrin in the range of 0.0398-1.990 μg (R2 = 0.999 3). Their average recoveries were 99.1 % (RSD = 1 %), 98.7 % (RSD = 2.4 %) and 97.1 % (RSD = 1.0 %), respectively. This method was steady with high precision and good repeatability and could be used for the determination of the 3 active ingredients and quality control of Wubishan capsule.
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References
V.P. Doyle, P.V. Oudemans, S.A. Rehner and A. Litt, PLoS One, 8, e62394 (2013); doi:10.1371/journal.pone.0062394.
Y. Huang, D. Nikolic, S. Pendland, B.J. Doyle, T.D. Locklear and G.B. Mahady, Pharm. Biol., 47, 18 (2009); doi:10.1080/13880200802397996.
Y. Feng, K.-F. Cheung, N. Wang, P. Liu, T. Nagamatsu and Y. Tong, Chin. Med., 4, 16 (2009); doi:10.1186/1749-8546-4-16.
S. Wang, J. Leng, Y. Xu and M. Feng, J. Zhejiang Univ. Sci. B, 14, 604 (2013); doi:10.1631/jzus.B1200295.
J.-N. Lai, H.-J. Chen, C.-C. Chen, J.-H. Lin, J.-S. Hwang and J.-D. Wang, Chin. Med., 12, 185 (2007).
M. Schumacher, C. Cerella, S. Reuter, M. Dicato and M. Diederich, Yale J. Biol. Med., 64, 79 (1997).
D. Liu, S.-H. Zeng, J.-J. Chen, Y.-J. Zhang, G. Xiao, L.-Y. Zhu and Y. Wang, Int. J. Mol. Sci., 14, 13559 (2013); doi:10.3390/ijms140713559.
E.I. Karchmer, Cult. Med. Psychiatry, 37, 14 (2003).
J.S. Haller Jr., Bull. N. Y. Acad. Med., 66, 233 (2005).
S. Kim, S. Shin, B. Hyun, H. Kong, S. Han, A. Lee, S. Lee and K. Kim, Immune Netw., 12, 181 (2012); doi:10.4110/in.2012.12.5.181.