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Improvement of Antioxidant Activity of Whey Protein Hydrolyzate by Conjugation with Glycosylation
Corresponding Author(s) : Baohua Kong
Asian Journal of Chemistry,
Vol. 26 No. 16 (2014): Vol 26 Issue 16
Abstract
This study was conducted to investigate the antioxidant activity of whey protein hydrolyzate by conjugation with glucosylation. The reaction conditions of whey protein hydrolyzates conjugated with glucose were optimized with the response surface methodology using the central composite rotatable design. The optimisation parameters studied were glucose concentration 8 %, temperature 94 °C and reaction time 3.2 h. Under this optimum condition, the reducing power, hydroxyl radical scavenging activity, scavenging of the ABTS radical, copper and iron chelating, thiobarbituric acid-reactive substances of whey protein hydrolyzates by conjugation with glucose through Maillard reaction are higher than whey protein hydrolyzates. The result showed that whey protein hydrolyzates by conjugation with glucose had high antioxidant activities.
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M. Hattori, Y. Aiba, K. Nagasawa and K. Takahashi, J. Food Sci., 61, 1171 (1996); doi:10.1111/j.1365-2621.1996.tb10954.x.
K. Nagasawa, K. Takahashi and M. Hattori, Food Hydrocoll., 10, 63 (1996); doi:10.1016/S0268-005X(96)80055-5.
C.M. Oliver, L.D. Melton and R.A. Stanley, Food Sci. Nutr., 46, 337 (2006).
L. Jiménez-Castaño, M. Villamiel, P.J. Martín-Alvarez, A. Olano, and R. López-Fandiño, Food Hydrocoll., 19, 831 (2005); doi:10.1016/j.foodhyd.2004.10.033.
E. Dickinson and V.B. Galazka, Food Hydrocoll., 5, 281 (1991); doi:10.1016/S0268-005X(09)80114-8.
C.A. Dunlap and G.L. Cote, J. Agric. Food Chem., 53, 419 (2005); doi:10.1021/jf049180c.
A. Kato, Y. Sasaki, R. Furuta and K. Kobayashi, Agric. Biol. Chem., 54, 107 (1990); doi:10.1271/bbb1961.54.107.
Y. Shu, S. Sahara, S. Nakamura and A. Kato, J. Agric. Food Chem., 44, 2544 (1996); doi:10.1021/jf950586m.
F. Chevalier, J.M. Chobert, C. Genot and T. Haertlé, J. Agric. Food Chem., 49, 5031 (2001); doi:10.1021/jf010549x.
K. Damianou and V. Kiosseoglou, Food Hydrocoll., 20, 793 (2006); doi:10.1016/j.foodhyd.2005.07.011.
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T.J. Wooster and M.A. Augustin, J. Colloid Interf. Sci., 303, 564 (2006); doi:10.1016/j.jcis.2006.07.081.
T.J. Wooster and M.A. Augustin, J. Colloid Interf. Sci., 313, 665 (2007); doi:10.1016/j.jcis.2007.04.054.
E.A. Peña-Ramos and Y.L. Xiong, J. Dairy Sci., 84, 2577 (2001); doi:10.3168/jds.S0022-0302(01)74711-X.
T. Juntachote, E. Berghofer, F. Bauer and S. Siebenhandl, Int. J. Food Sci. Technol., 41, 121 (2006); doi:10.1111/j.1365-2621.2005.00987.x.
G.C. Yen and P.D. Duh, J. Am. Oil Chem. Soc., 70, 383 (1993); doi:10.1007/BF02552711.
R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans, Free Radic. Biol. Med., 26, 1231 (1999); doi:10.1016/S0891-5849(98)00315-3.
J.C. Lee, H.R. Kim, J. Kim and Y.S. Jang, J. Agric. Food Chem., 50, 6490 (2002); doi:10.1021/jf020388c.
T.C.P. Dinis, V.M.C. Madeira and L.M. Almeida, Arch. Biochem. Biophys., 315, 161 (1994); doi:10.1006/abbi.1994.1485.
E.A. Decker and H.O. Hultin, J. Food Sci., 55, 947 (1990); doi:10.1111/j.1365-2621.1990.tb01571.x.
B.H. Kong and Y.L. Xiong, J. Agric. Food Chem., 54, 6059 (2006); doi:10.1021/jf060632q.
J.S. Kim and Y.S. Lee, Food Chem., 116, 227 (2009); doi:10.1016/j.foodchem.2009.02.038.
F. Chevalier, J.M. Chobert, Y. Popineau, M.G. Nicolas and T. Haertlé, Int. Dairy J., 11, 145 (2001); doi:10.1016/S0958-6946(01)00040-1.
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J. Saha, A. Biswas, A. Chhetri and P.K. Sarkar, Food Chem., 129, 507 (2011); doi:10.1016/j.foodchem.2011.04.108.
C. Beermann, M. Euler, J. Herzberg and B. Stahl, Eur. Food Res. Technol., 229, 637 (2009); doi:10.1007/s00217-009-1093-1.
A. Jimenez-Escrig, M. Alaiz, J. Vioque and P. Ruperez, Eur. Food Res. Technol., 230, 655 (2010); doi:10.1007/s00217-009-1203-0.
A. Saiga, S. Tanabe and T. Nishimura, J. Agric. Food Chem., 51, 3661 (2003); doi:10.1021/jf021156g.
C. Torres-Fuentes, M. Alaiz and J. Vioque, Food Chem., 129, 485 (2011); doi:10.1016/j.foodchem.2011.04.103.