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Vol. 26 No. 16 (2014): Vol 26 Issue 16

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Determination of Four Flavonoids in Cuscuta chinensis Lam. by HPLC with Ultrahigh Pressure-Assisted Cloud Point Extraction

https://doi.org/10.14233/ajchem.2014.16318
Ronghua Zhu
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, Shandong Province, P.R. China
Feng Liu
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, Shandong Province, P.R. China
Wei Liu
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, Shandong Province, P.R. China
Shuqin Zhang
College of Chemistry and Material Science, Shandong Agricultural University, 61 Daizong Street, Taian 271018, Shandong Province, P.R. China
Xiao Wang
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, Shandong Province, P.R. China ;College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Taian 271018, Shandong Province, P.R. China
Luqi Huang
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, Shandong Province, P.R. China

Corresponding Author(s) : Feng Liu

liufeng8109@163.com

Asian Journal of Chemistry, Vol. 26 No. 16 (2014): Vol 26 Issue 16

Abstract

In this paper, a novel two-step extraction technique combining ultrahigh pressure-assisted micellar extraction with cloud point extraction was presented for the extraction and pre-concentration of four flavonoids (hyperoside, quercitrin, quercetin and kaempferol) from the seeds of Cuscuta chinensis Lam. (Tu-si-zi) for the first time. Various experimental conditions were investigated to optimize the extraction process. Under the optimal conditions, i.e., 5 % (w/w) Triton X-100 solution, solid/liquid ratio of 1:30 (g/mL), with 100 MPa for only 1 min, the extraction efficiency of four flavonoids reached the highest value. The pre-concentration of four flavonoids by cloud point extraction were established as follows: the solution was incubated in a thermostatic water bath at 65 °C for 5 min and 15 % NaCl was added into the solution to facilitate the phase separation and increase the pre-concentration factor during the cloud point extraction process. The results indicated that the proposed method, combining two different and efficient techniques, offers satisfactory analytical features in terms of repeatability and reproducibility.

Keywords

Ultrahigh pressure extraction Cloud point extraction Flavonoids Triton X-100 HPLC
Zhu, R., Liu, F., Liu, W., Zhang, S., Wang, X., & Huang, L. (2014). Determination of Four Flavonoids in Cuscuta chinensis Lam. by HPLC with Ultrahigh Pressure-Assisted Cloud Point Extraction. Asian Journal of Chemistry, 26(16), 5058–5062. https://doi.org/10.14233/ajchem.2014.16318
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