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Vol. 26 No. 16 (2014): Vol 26 Issue 16

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A Fast HPLC Quantitative Determination of Ligustrazine in Rhizome of Ligusticum chuanxiong

https://doi.org/10.14233/ajchem.2014.16303
Jin-Liang Liu
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R. China ;Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of P.R. China, Chengdu 611130, P.R. China
Shun-Lin Zheng
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R. China
Qiao-Jia Fan
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, P.R. China
Ji-Chao Yuan
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R. China ;Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of P.R. China, Chengdu 611130, P.R. China
Shi-Min Yang
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R. China
Fan-Lei Kong
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R. China ;Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of P.R. China, Chengdu 611130, P.R. China

Corresponding Author(s) : Ji-Chao Yuan

yuanjichao5@163.com

Asian Journal of Chemistry, Vol. 26 No. 16 (2014): Vol 26 Issue 16

Abstract

A simple and quick method is described for the determination of ligustrazine in rhizomes of Ligusticum chuanxiong. The ligustrazine in the sample was extracted using methanol and analyzed by reversed-phase high performance liquid chromatography. Chromatography separation was performed using Agilent 1100 series HPLC system with a waters symmetry C18 column and isocratic elution with a mixture of three solvents: acetonitrile, methanol and 1 % aqueous acetic acid (20: 40: 40, v/v/v). The effluent was monitored using a VWD detector set at 294 nm. The average recoveries were 84.93 - 88.65 % (n = 3). The limits of detection and quantification were 0.044 and 0.133 μg mL-1, respectively. The method has been successfully applied to the analysis of L. chuanxiong samples. For 37 samples, the ligustrazine content varied from 0.22 to 1.06 mg g-1.

Keywords

Ligustrazine HPLC Ligusticum chuanxiong Quantitative determination Method Validation
Liu, J.-L., Zheng, S.-L., Fan, Q.-J., Yuan, J.-C., Yang, S.-M., & Kong, F.-L. (2014). A Fast HPLC Quantitative Determination of Ligustrazine in Rhizome of Ligusticum chuanxiong. Asian Journal of Chemistry, 26(16), 5026–5028. https://doi.org/10.14233/ajchem.2014.16303
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References
  1. C. Zhang, M. Qi, Q. Shao, S. Zhou and R. Fu, J. Pharm. Biomed. Anal., 44, 464 (2007); doi:10.1016/j.jpba.2007.01.024.
  2. Q.H. Yang, Y. Liang, Q. Xu, Y. Zhang, L. Xiao and L.Y. Si, Phytomedicine, 18, 1148 (2011); doi:10.1016/j.phymed.2011.05.003.
  3. R. Yan, S.L. Li, H.S. Chung, Y.K. Tam and G. Lin, J. Pharm. Biomed. Anal., 37, 87 (2005); doi:10.1016/j.jpba.2004.09.054.
  4. Y.- Su, Z.-y. Fu, C.- Quan and W.- Wang, Nonferr. Metal Soc., 16, s393 (2006); doi:10.1016/S1003-6326(06)60218-5.
  5. Y. Jin, T. Liang, Q. Fu, Y.S. Xiao, J.T. Feng, Y.X. Ke and X.M. Liang, J. Chromatogr. A, 1216, 2136 (2009); doi:10.1016/j.chroma.2008.04.010.
  6. H. Zheng, X.L. Chen, Z.X. Han, Z. Zhang, S.Y. Wang and Q.L. Xu, Burns, 31, 453 (2005); doi:10.1016/j.burns.2004.10.023.
  7. X.P. Zhang, J. Jiang, Q.H. Cheng, Q. Ye, W.J. Li, H. Zhu and J.Y. Shen, Hepatobiliary Pancreat. Dis. Int., 10, 632 (2011); doi:10.1016/S1499-3872(11)60107-0.
  8. F. Zhang, C. Ni, D. Kong, X. Zhang, X. Zhu, L. Chen, Y. Lu and S. Zheng, Toxicol. Appl. Pharmacol., 265, 51 (2012); doi:10.1016/j.taap.2012.09.016.
  9. Y. Xue, C.R. Tie, J. Li, T. Tian and Q.X. Li, Eur. J. Pharmacol., 665, 8 (2011); doi:10.1016/j.ejphar.2011.05.004.
  10. H.J. Wu, J. Hao, S.Q. Wang, B.L. Jin and X.B. Chen, Eur. J. Pharmacol., 674, 365 (2012); doi:10.1016/j.ejphar.2011.10.046.
  11. J. Shi, Q. Liu, Y. Wang and G. Luo, Pharmacol. Biochem. Behav., 96, 449 (2010); doi:10.1016/j.pbb.2010.07.004.
  12. X.H. Liu, J. Li, Q.X. Li, Y.X. Ai and L. Zhang, Environ. Toxicol. Pharmacol., 26, 49 (2008); doi:10.1016/j.etap.2008.01.006.
  13. S. Gao, Z.W. Chen, H. Zheng and X.L. Chen, Burns, 33, 321 (2007); doi:10.1016/j.burns.2006.07.002.
  14. C. Gao, H. Peng, S. Wang and X. Zhang, Burns, 38, 102 (2012); doi:10.1016/j.burns.2011.04.022.
  15. C. Gao, L. Feng, Y.P. Li and Y. Cheng, Transplant. Proc., 39, 3415 (2007); doi:10.1016/j.transproceed.2007.04.026.
  16. H. Fu, J. Li, Q.X. Li, L. Xia and L. Shao, Eur. J. Pharmacol., 563, 197 (2007); doi:10.1016/j.ejphar.2007.02.015.
  17. L. Feng, Y. Xiong, F. Cheng, L. Zhang, S. Li and Y. Li, Transplant. Proc., 36, 1949 (2004); doi:10.1016/j.transproceed.2004.07.050.
  18. L. Feng, N. Ke, F. Cheng, Y. Guo, S. Li, Q. Li and Y. Li, J. Surg. Res., 166, 298 (2011); doi:10.1016/j.jss.2009.04.005.
  19. G.H. Lu, K. Chan, K. Leung, C.L. Chan, Z.Z. Zhao and Z.H. Jiang, J. Chromatogr. A, 1068, 209 (2005); doi:10.1016/j.chroma.2005.01.082.
  20. X.F. Xue, J.H. Zhou, L.M. Wu, L.H. Fu and J. Zhao, Food Chem., 115, 715 (2009); doi:10.1016/j.foodchem.2008.12.003.
  21. L. Xie, X.-N. Li, D.-X. Jiang and D. Zhang, J. Pharmaceutical Anal., 1, 297 (2011); doi:10.1016/j.jpha.2011.09.004.
  22. J.-i. Wakamatsu, H. Odagiri, T. Nishimura and A. Hattori, Meat Sci., 82, 139 (2009); doi:10.1016/j.meatsci.2008.12.011.
  23. N. Tavakoli, J. Varshosaz, F. Dorkoosh and M.R. Zargarzadeh, J. Pharm. Biomed. Anal., 43, 325 (2007); doi:10.1016/j.jpba.2006.06.002.
  24. C.I. Rodrigues, L. Marta, R. Maia, M. Miranda, M. Ribeirinho and C. Máguas, J. Food Compos. Anal., 20, 440 (2007); doi:10.1016/j.jfca.2006.08.005.
  25. S. Kongkiatpaiboon, S. Mikulicic, V. Keeratinijakal, H. Greger and W. Gritsanapan, Ind. Crops Prod., 43, 648 (2013); doi:10.1016/j.indcrop.2012.08.014.
  26. M.N. Irakli, V.F. Samanidou, C.G. Biliaderis and I.N. Papadoyannis, Food Chem., 134, 1624 (2012); doi:10.1016/j.foodchem.2012.03.046.
  27. W.H. Organization, International Conference on Harmonization Guideline (Validation of Analytical Procedure-Methodology), vol. 14(3), p. 153 (2000).
  28. W. Weiyu, S. Teng, X. Huinan, Z. Jianfang, H. Jianming, H. Zhaochang and C. Jia, Chinese J. Chromatogr., 23, 276 (2005).
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