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Vol. 26 No. 16 (2014): Vol 26 Issue 16

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Simultaneous Determination of Four Classes of Acanthopanax giraldii Hormone by HPLC Method

https://doi.org/10.14233/ajchem.2014.16293
Shun-Lin Zheng
College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, P.R. China
Jin-Liang Liu
College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, P.R. China
Rui Gu
National Medical College, Chengdu University of Traditional Chinese Medicine, Chengdu 611131, P.R. China
Meng-Liang Tian
College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, P.R. China
Shi-Hong Zhongbi
Pharmaceutical School, Chengdu Medical College, Chengdu 610083, P.R. China
Yun Cao
National Medical College, Chengdu University of Traditional Chinese Medicine, Chengdu 611131, P.R. China

Corresponding Author(s) : Rui Gu

664893924@qq.com

Asian Journal of Chemistry, Vol. 26 No. 16 (2014): Vol 26 Issue 16

Abstract

A simple and quick method is described for the confirmation of hormones in annual branches of Acanthopanax giraldii. The hormones in the samples were extracted by using methanol and analyzed by HPLC. Chromatography separation was performed by using Agilent 1100 series HPLC system with symmetry C18 column and gradient elution with a mixture of two solvents: methanol and 1 % aqueous acetic acid. The effluent was monitored by a VWD detector set at 254 nm. The average recoveries of zeatin, gibberellins, indole acetic acid and abscisic acid were 99.43, 97.64, 93.16 and 95.28, respectively (n = 3). The limits of detection and quantification were 0.044 and 0.133 μg mL-1, respectively. The method has been successfully applied to analysis of 64 samples, zeatin, gibberellins, indole acetic acid and abscisic acid content of leaves ranged from 3.57-84.83, 0.00-43.09, 0.00-92.51 and 0.00-53.78 ng g-1, the zeatin, gibberellins, indole acetic acid and abscisic acid content of rinds ranged from 3.71-38.91, 0.00-34.36, 0.00-6.57 and 0.00-22.07 ng g-1.

Keywords

Hormones HPLC Acanthopanax giraldii Quantitative determination Method Validation
Zheng, S.-L., Liu, J.-L., Gu, R., Tian, M.-L., Zhongbi, S.-H., & Cao, Y. (2014). Simultaneous Determination of Four Classes of Acanthopanax giraldii Hormone by HPLC Method. Asian Journal of Chemistry, 26(16), 5011–5014. https://doi.org/10.14233/ajchem.2014.16293
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References
  1. R. Chao-Qin, L. Yuan, M. Qing-Yan and L. Chao, West Chin. J. Pharm. Sci., 23, 602 (2008).
  2. L. Ying, Y. Wei, L. Chao and L. Yuan, Lishizhen Med. Mat. Med. Res., 20, 1617 (2009).
  3. L. Ying, L. Yuan, Y. Wei, L. Yong-Heng and R. Ye, West Chin. J. Pharm. Sci., 25, 339 (2010).
  4. W. Zhu-Wei, Z. Qing-Hai, Z. Li-Xia and S. Yu-Qing, Chin. J. Pharm. Anal., 25, 765 (2005).
  5. L. Yuan, M. Qing-Yan and R. Chao-Qin, Chin. J. Pharm. Anal., 27, 1586 (2007).
  6. M. Xiu-Ying and D. Yue-Lan, Pharmacol. Clin. Chinese Mater. Med., 15, 18 (1999).
  7. Z. Ou-Yang and L. Qi-Cai, Chin. Med. Pharmacy, 2, 23 (2012).
  8. C. Ping, Z. Li-Xia and C. Xiu-Juan, Chinese Pharmaceut. J., 28, 351 (1993).
  9. J. Zhi-Quan, Z. Chong-Jie, W. Cheng-Xiao, Y. Pei-Quan and L. Guang-Xi, West Chin. J. Pharm. Sci., 8, 211 (1993).
  10. A.A. Kotov and L.M. Kotova, J. Plant Physiol., 156, 438 (2000); doi:10.1016/S0176-1617(00)80156-X.
  11. L. Arigita, B. Fernández, A. González and R.S. Tamés, Plant Physiol. Biochem., 43, 161 (2005); doi:10.1016/j.plaphy.2005.01.012.
  12. R.M. Suzuki, G.B. Kerbauy and G.R. Zaffari, J. Plant Physiol., 161, 929 (2004); doi:10.1016/j.jplph.2003.11.001.
  13. M. Iqbal and M. Ashraf, Environ. Exp. Bot., 86, 76 (2013); doi:10.1016/j.envexpbot.2010.06.002.
  14. L. Arrom and S. Munne-Bosch, J. Plant Physiol., 169, 1542 (2012); doi:10.1016/j.jplph.2012.06.012.
  15. P. Rui-Chi, W. Xiao-Qing and L. Niang-Hui, Higher Education Press (China), pp. 167-204 (2008).
  16. W. Xue-Kui, Z. Wen-Hua, H. Zai-Bin, L. Xiu-Ru, Z. Yu-Qiong and Z. Shuang-Ming, Higher Education Press (China), pp. 93-96 (2006).
  17. W. Xue-Kui, Z. Wen-Hua, H. Zai-Bin, L. Xiu-Ru, Z. Yu-Qiong and Z. Shuang-Ming, Higher Education Press (China), p. 250 (2006).
  18. Z. Ma, L. Ge, A.S. Lee, J.W. Yong, S.N. Tan and E.S. Ong, Anal. Chim. Acta, 610, 274 (2008); doi:10.1016/j.aca.2008.01.045.
  19. V. Gupta, M. Kumar, H. Brahmbhatt, C.R. Reddy, A. Seth and B. Jha, Plant Physiol. Biochem., 49, 1259 (2011); doi:10.1016/j.plaphy.2011.08.004.
  20. H. Chen, X.F. Guo, H.S. Zhang and H. Wang, J. Chromatogr. B, 879, 1802 (2011); doi:10.1016/j.jchromb.2011.05.002.
  21. Z. Shun-Lin, W. Xi-Yao, M. Jun, Y. Ji-Chao and L. Shou-Cheng, Plant Nutr. Fert. Sci., 14, 515 (2008).
  22. L. Qian-Qian, W. Tao, C. Ping, Y. Wei-Dong and L. Shan-Shan, Chinese J. Grassland, 34, 117 (2012).
  23. Z. Qing-Qian, C. Hou-Bin, L. Cai-Hao and L. Jian-Guo, J. Fruit Sci., 23, 145 (2006).
  24. Y. Izumi, A. Okazawa, T. Bamba, A. Kobayashi and E. Fukusaki, Anal. Chim. Acta, 648, 215 (2009); doi:10.1016/j.aca.2009.07.001.
  25. World Health Organization, International Conference on Harmonization Guideline (Validation of Analytical Procedure-Methodology), vol. 14(3), p. 153 (2000).
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