Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis

Renming Yang; Honglun Wang; Gang Li; Yourui Suo; Jinmao You

doi:10.14233/ajchem.2014.16256

Issue

Vol. 26 No. 15 (2014): Vol 26 Issue 15

Issue Published : July 16, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis

https://doi.org/10.14233/ajchem.2014.16256

Renming Yang

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China

Honglun Wang

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China

Gang Li

Life School of Yantai University, Yantai 264005, P.R. China

Yourui Suo

Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China

Jinmao You

College of Chemistry Science, Qufu Normal University, Qufu 273165, Shandong Province, P.R. China

Corresponding Author(s) : Honglun Wang

hlwang@nwipb.cas.cn

Asian Journal of Chemistry, Vol. 26 No. 15 (2014): Vol 26 Issue 15
Article Published : July 16, 2014

Abstract

In this study, a central composite design based on response surface methodology was used to optimize ultrasonic-assistant extraction of flavonoids from Trigonella foenum-graecum L. The optimal conditions to obtain the highest yield of total flavonoids were as follows: ultrasonic time of 80 min, temperature of 48 °C and liquid to solid ratio of 20 g/mL, where 1.03 % yield was predicted. After extraction process optimization, a method for the analysis of flavonoids in Trigonella foenum-graecum L. by HPLC-DAD-MS was developed. The LOD and LOQ of these 4 compounds range from 50.94 to 70.31 ng/mL and 179.63 to 208.85 ng/mL, respectively. The established HPLC-DAD-MS method was proven to be useful for the quantitative and qualitative analysis of flavonoid constituents in fenugreek seed. This is the first report on preparation, identification and determination of flavonoids in fenugreek seed by different method and which would provide a basis for medicinal and edible use of fenugreek.

Keywords

Fenugreek Flavonoids Response surface methodology Ultrasound-assisted extraction Quantitative analysis

Yang, R., Wang, H., Li, G., Suo, Y., & You, J. (2014). Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis. Asian Journal of Chemistry, 26(15), 4830–4834. https://doi.org/10.14233/ajchem.2014.16256

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References

B. Kaufmann, S. Rudaz, S. Cherkaoui, J.L. Veuthey and P. Christen, Phytochem. Anal., 18, 70 (2007); doi:10.1002/pca.954.

S. Rayyan, T. Fossen and Ø.M. Andersen, J. Agric. Food Chem., 58, 7211 (2010); doi:10.1021/jf100848c.

G.R. Wang, W.Z. Tang, Q.Q. Yao, H. Zhong and Y.J. Liu, J. Nat. Med., 64, 358 (2010).

M.A. Pollard, B. Eder, P. Fischer and E.J. Windhab, Carbohydr. Polym., 79, 70 (2010); doi:10.1016/j.carbpol.2009.07.028.

C.L. Gopu, S.S. Gilda, A.R. Paradkar and K.R. Mahadik, Acta. Chromatogr., 20, 709 (2008); doi:10.1556/AChrom.20.2008.4.15.

M.V. Vijayakumar and M.K. Bhat, Phytother. Res., 22, 500 (2008); doi:10.1002/ptr.2351.

P. Sowmya and P. Rajyalakshmi, Plant Foods Hum. Nutr., 53, 359 (1999); doi:10.1023/A:1008021618733.

Y. Han, S. Nishibe, Y. Noguchi and Z. Jin, Phytochemistry, 58, 577 (2001); doi:10.1016/S0031-9422(01)00273-4.

M.Y. Shang, S.Q. Cai, J. Han, J. Li, Y.Y. Zhao and J.H. Zheng, China J. Chin. Mater. Med., 23, 614 (1998).

J.J. Shan, J.W. Ren, C.M. Wu and Y.M. Zhao, Chin. Pharm. J., 43, 1457 (2008).

P. Dixit, S. Ghaskadbi, H. Mohan, L.R. Susan and P. Rajasekaran, Phytother. Res., 19, 977 (2005); doi:10.1002/ptr.1769.

G.W. Zhang, L. He and M.M. Hu, Innov. Food Sci. Emerg. Technol., 12, 18 (2011); doi:10.1016/j.ifset.2010.12.003.

J.F. Song, D.J. Li, C.Q. Liu and Y. Zhang, Innov. Food Sci. Emerg. Technol., 12, 282 (2011); doi:10.1016/j.ifset.2011.03.001.

M.S. Liza, R.A. Rahman, B. Mandana, S. Jinap, A. Rahmat, I.S.M. Zaidul and A. Hamid, Food Bioproducts Process., 88, 319 (2010); doi:10.1016/j.fbp.2009.02.001.

D. Bas and I.H. Boyaci, J. Food Eng., 78, 836 (2007); doi:10.1016/j.jfoodeng.2005.11.024.

W. Xiao, L. Han and B. Shi, Sep. Sci. Technol., 43, 671 (2008); doi:10.1080/01496390701812509.

K.-W. Kong, A.R. Ismail, S.-T. Tan, K.M. Nagendra Prasad and A. Ismail, Int. J. Food Sci. Technol., 45, 1739 (2010); doi:10.1111/j.1365-2621.2010.02335.x.

S.C. Liu, F. Yang, C.H. Zhang, H. W. Ji, P.Z. Hong and C.J. Deng, J. Supercrit. Fluids, 48, 9 (2009); doi:10.1016/j.supflu.2008.09.013.

Issue

Vol. 26 No. 15 (2014): Vol 26 Issue 15

Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis

Corresponding Author(s) : Honglun Wang

Abstract

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