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Vol. 26 No. 15 (2014): Vol 26 Issue 15

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Factors Influencing Crude Oil Biodegradation by Pseudomonas sp. DG17

https://doi.org/10.14233/ajchem.2014.16149
Fei Hua
College of Water Sciences, Beijing Normal University, No. 19, XinJieKouWai St., HaiDian District, Beijing 100875, P.R. China
Hong Qi Wang
College of Water Sciences, Beijing Normal University, No. 19, XinJieKouWai St., HaiDian District, Beijing 100875, P.R. China

Corresponding Author(s) : Hong Qi Wang

whongqi310@sohu.com

Asian Journal of Chemistry, Vol. 26 No. 15 (2014): Vol 26 Issue 15

Abstract

Pseudomonas is unique in the soil with the ability to degrade petroleum susbstrate. In the present work, effect of environmental factors including, crude oil concentration, salinity, pH and nutrient on the biodegradation of crude oil as well as the biodegradability of different alkanes by Pseudomonas sp. DG17 were investigated. The results showed that inoculum cell content had positive and crude oil concentration, salinity, pH and biosurfactant had negative effect. Pseudomonas sp. DG17 requires an optimal substrate concentration was 500 mg L-1, the optimum inoculation cell content (OD600) was 1, the optimum salinity was 1 %, the optimum pH range was 6-8 and the optimum biosurfactant additon was 10 mg L-1. In additon, high concentraton of biosurfactant had toxice effect on the biodegradation of crude oil by DG17. Moreover, the biodegradability of C12 to C19 of saturable alkanes and cell growh of Pseudomonas sp. DG17 decreased in order of increasing molecular weight.

Keywords

Pseudomonas Crude oil Biodegradation Factors Alkanes
Hua, F., & Qi Wang, H. (2014). Factors Influencing Crude Oil Biodegradation by Pseudomonas sp. DG17. Asian Journal of Chemistry, 26(15), 4637–4642. https://doi.org/10.14233/ajchem.2014.16149
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