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Studies on Purification Process of Total Saponins in Radix Astragali with Resin and Structural Identification of Compounds
Corresponding Author(s) : Ling-Yan Jian
Asian Journal of Chemistry,
Vol. 26 No. 15 (2014): Vol 26 Issue 15
Abstract
This study reports the process conditions for purification and enrichment of total saponins in Radix Astragali with macroporous adsorption resin. Reflux method was used to obtain Radix Astragali extract; spectrophotometry was used to determine the content of total saponins in Radix Astragali and to study the selection of macroporous adsorption resin types as well as purification conditions. D101 resin had a relatively strong static adsorption-desorption capacity. The enrichment and purification effects of total saponins in Radix Astragali were optimal when the concentration of total saponins of Radix Astragali was selected as 40 mg/mL, sample adsorption rate was selected as 3 BV/h, adsorption column with a diameter height ratio of 1:10 was used and 80 % ethanol was used as the eluent; pH value of drug solution had no effect on the results. Under the experimental conditions, D101 resin is suitable for the extraction, isolation and purification of total saponins in Radix Astragali.
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References
Jiangsu New Medical College, Dictionary of Chinese Medicine, pp. 2036-2040 (1997).
Z.Z. Cao, J.H. Yu, L.X. Gan and Y.Q. Chen, Acta Chim. Sinica, 43, 581 (1985).
M. Hirotani, Y. Zhou, H. Lui and T. Furuya, Phytochemistry, 36, 665 (1994); doi:10.1016/S0031-9422(00)89793-9.
M. Hirotani, Y. Zhou, H. Rut and T. Furuya, Phytochemistry, 37, 1403 (1994); doi:10.1016/S0031-9422(00)90420-5.
Hand book of Flora Medicine Effective Constituents, p. 586 (1986).
W.B. Smith and S. Chiranjeevi, J. Phys. Chem., 70, 3505 (1966); doi:10.1021/j100883a023.
W.-L. Li, Y.-P. Wang, Y.-B. Ji, Y.-Q. Fang and P. Zhao, China J. Chinese Mater. Med., 396 (2007).
S.W. Huang and W.-H. Wu, J. Chinese Med. Mater., 1352 (2006).
C.Q. Song, Z.-R. Zheng, D. Liu, Z.-B. Hu and W.Y. Sheng, Acta Botanica Sinica, 39, 1169 (1997).
O. Ghribi, D.A. DeWitt, M.S. Forbes, A. Arad, M.M. Herman and J. Savory, J. Aizheimers Dis., 3, 387 (2001).
Y.-S. Wang and Y. Wang, China J. Chinese Mater. Med., 961 (2006).
L. Ping, Tianjin Pharm., 9 (2002).
L. Bo-ting and L. Xiao-jin, Chin. Tradit. Herbal Drugs, 42 (1990).
Y.P. Su, Q.-H. Wang, Y.-B. Xun, Z.Z. Bai, G.Q. Zhao and J.J. Zuo, Heilongjiang Med. J., 850 (2012).
L. Tang, Y. Ma, C.-Q. Cai, J.-S. Li, Y. Liu, B. Wang and X. Yao, Acta Chinese Med. Pharmacol., 51 (2011).
P.-H. Xu, Y. Gao, X.-Q. Zhang, K. Xu and L. Zhang, Chinese Tradit. Patent Med., 390 (2009).