Analysis of Phenolic Compounds and Determination of Loureirin B in Dragon's Blood by LC-MS and High Performance Capillary Electrophoresis

Tian-Ming Yang; Li-Ping Wang; Hai-Yan Fu; Yuan-Bin She; Can Huang; Yan Hu; Hao Zhang

doi:10.14233/ajchem.2014.15922

Issue

Vol. 26 No. 13 (2014): Vol 26 Issue 13

Issue Published : June 23, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Analysis of Phenolic Compounds and Determination of Loureirin B in Dragon's Blood by LC-MS and High Performance Capillary Electrophoresis

https://doi.org/10.14233/ajchem.2014.15922

Tian-Ming Yang

Third Class Laboratory About Ethno-medicine of State Administration of Traditional Chinese Medicine; College of Pharmacy, South-central University for Nationalities, 182 Minyuan Road, Hongshan District, Wuhan 430074, P.R. China

Li-Ping Wang

Hai-Yan Fu

Yuan-Bin She

Key Laboratory of Catalysis and Materials Science of the State Ethnic Affairs Commission & Ministry of Education; College of Chemistry and Materials Science, South-Central University for Nationalities, 182 Minyuan Road, Hongshan District, Wuhan 430074, P.R. China

Can Huang

Yan Hu

Hao Zhang

Corresponding Author(s) : Hai-Yan Fu

fuhaiyan@mail.scuec.edu.cn

Asian Journal of Chemistry, Vol. 26 No. 13 (2014): Vol 26 Issue 13
Article Published : June 26, 2014

Abstract

The high performance liquid chromatography-mass spectrometry (HPLC-MS) and the high performance capillary electrophoresis (HPCE) were applied to study the phenolic compounds of Dragon's blood extracts. Ten compounds were identified from Dragon's blood extracts. Moreover, under the optimized HPCE conditions, linear calibration curve of Loureirin B was obtained for the system, the accuracy of the method was validated by spiked test and the average recoveries arrive at 98.1 %. The precision and repeatability were also investigated by the relative standard deviations (RSDs) of the prepared samples in quintuplicate. The relative standard deviations of the HPCE intensity and the migration time were 3.8 % and 1.3 %, respectively. Successful separation and satisfactory quantitative results for Loureirin B in complicated Dragon's blood were obtained at pH 9.6. The developed method based on HPLC-MS and HPCE for identification of phenolic compounds and quantification of Loureirin B in complex Dragon's blood is simple and effective and the rusults can be applied to the componential analysis and quality control of herbs.

Keywords

Dragon's blood Phenolic compounds Loureirin B LC-MS HPCE

Yang, T.-M., Wang, L.-P., Fu, H.-Y., She, Y.-B., Huang, C., Hu, Y., & Zhang, H. (2014). Analysis of Phenolic Compounds and Determination of Loureirin B in Dragon’s Blood by LC-MS and High Performance Capillary Electrophoresis. Asian Journal of Chemistry, 26(13), 3774–3778. https://doi.org/10.14233/ajchem.2014.15922

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References

Q.Y. Zhang, H. Zhu, H.Y. Chen and Y.Q. Hu, Acta Acad. Med. CPAPF, 13, 69 (2003).
China Pharmacopoeia Committee, Pharmacopoeia of the People’s Republic of China, Chinese Science and Technology Press: Beijing, vol. 1, p.133 (2010).
The Editorial Committee, Materia medica of china, Shanghai Science and Technology Publishing House: Shanghai, vol. 8, p.455 (1999).
A.G. González, F. León and L. Sánchez-Pinto, J.I. Padrón and J. Bermejo, J. Nat. Prod., 63, 1297 (2000); doi:10.1021/np000085h.
J.L. Wang, X.C. Li, D.F. Jiang, P. Ma and C.R. Yang, Acta Botanica Yunnanica, 17, 336 (1995).
Z.H. Zhou, J.L. Wang and C.R. Yang, Acta Pharmacol. Sin., 36, 200 (2001).
W.J. Lu, X.F. Wang, J.Y. Chen, Y. Lv, N. Wu, W.J. Kang and Q.T. Zheng, Acta Pharmacol. Sin., 33, 755 (1998).
U. Himmelreich, M. Masaoud, G. Adam and H. Ripperger, Phytochemistry, 39, 949 (1995); doi:10.1016/0031-9422(95)00044-8.
M. Masaoud, H. Ripperger, U. Himmelreich and G. Adam, Phytochemistry, 38, 751 (1995); doi:10.1016/0031-9422(94)00718-9.
Q.A. Zheng, H.Z. Li, Y.J. Zhang and C.R. Yang, Helv. Chim. Acta, 87, 1167 (2004); doi:10.1002/hlca.200490106.
Z.H. Zhou, J.L. Wang, C.R. Yang, Chinese Tradit. Herbal Drugs, 32, 484 (2001).
F. Liu, R.J. Dai, Y.L. Deng, W.W. Meng and X.Y. Jin, China Pharmacy, 21, 1437 (2010).
D. Gupta and R.K. Gupta, BMC Complement. Altern. Med., 11, 13 (2011); doi:10.1186/1472-6882-11-13.
D. Gupta, B. Bleakley and R.K. Gupta, J. Ethnopharmacol., 115, 361 (2008); doi:10.1016/j.jep.2007.10.018.
N. Xin, Y.J. Li, Y. Li, R.J. Dai, W.W. Meng, Y. Chen, M. Schlappi and Y.-L. Deng, J. Ethnopharmacol., 135, 510 (2011); doi:10.1016/j.jep.2011.03.063.M. Schlappi
T. Yi, H.B. Chen, Z.Z. Zhao, Z.L. Yu and Z.H. Jiang, J. Ethnopharmacol., 133, 796 (2011); doi:10.1016/j.jep.2010.11.008.
J.Y. Deng, Y.M. Li and W.R. Fang, Chin. J. Nat. Med., 4, 373 (2006).
W.R. Fang, Y.N. Li and J.Y. Deng, Chin. J. Clin. Pharmacol. Ther., 10, 1020 (2005).
J.Y. Ren, Q.B. Zhang and W.H. Jiang, ITCM, 23, 31 (2006).
Z.X. Wang, LiShiZhen Med. Mater. Medica Res., 16, 269 (2005).
Y. Li, W. Xiao, J.P. Qin, X. Lin and Z.Z. Wang, Chinese J. Exp. Tradit. Med Formulae, 18, 45 (2012).
Y. Li, W. Xiao, J. Qin, Y. Guo and Q. Zhu, China J. Chinese Mater. Med., 37, 929 (2012).
H. Li and X.L. Sun, Chinese Pharm. Affairs, 24, 696 (2010).
Y.H. Wang, S.Y. Mu, W. Wang and G. Hang, LiShiZhen Med. Mater. Med. Res., 11, 1073 (2000).
Q.L. Song and S.F. Hu, China Pharmacist, 12, 1314 (2009).
D.F. Chen and Q.S. Song, Chin. Tradit. Herbal Drugs, 36, 1545 (2005).
S.L. Sun, H.M. Mi, S.Q. Wu, Q.F. Yao, Y.M. Hu and Y.S. Zheng, Chin. Tradit. Herbal Drugs, 33, 1033 (2002).
W.J. Gong, Y.H. Cao and Y. Wang, Chromatographia, 66, 767 (2007); doi:10.1365/s10337-007-0406-4.
K. Ichikawa, M. Kitaoka, M. Taki, S. Takaishi, Y. Lijima, M. Boriboon and T. Akiyama, Planta Med., 63, 540 (1997); doi:10.1055/s-2006-957759.
G. Nasini, L. Camarda and L. Merlini, Heterocycles, 20, 39 (1983); doi:10.3987/R-1983-01-0039.
Y.Q. Hu, P.F. Tu, R.Y. Li, Z. Wan and R.L. Wang, Chin. Tradit. Herbal Drugs, 32, 104 (2001).
D. Veselá, R. Marek, K. Ubik, K. Lunerová, V. Sklenář and V. Suchý, Phytochemistry, 61, 967 (2002); doi:10.1016/S0031-9422(02)00354-0.

References

Q.Y. Zhang, H. Zhu, H.Y. Chen and Y.Q. Hu, Acta Acad. Med. CPAPF, 13, 69 (2003).

China Pharmacopoeia Committee, Pharmacopoeia of the People’s Republic of China, Chinese Science and Technology Press: Beijing, vol. 1, p.133 (2010).

The Editorial Committee, Materia medica of china, Shanghai Science and Technology Publishing House: Shanghai, vol. 8, p.455 (1999).

A.G. González, F. León and L. Sánchez-Pinto, J.I. Padrón and J. Bermejo, J. Nat. Prod., 63, 1297 (2000); doi:10.1021/np000085h.

J.L. Wang, X.C. Li, D.F. Jiang, P. Ma and C.R. Yang, Acta Botanica Yunnanica, 17, 336 (1995).

Z.H. Zhou, J.L. Wang and C.R. Yang, Acta Pharmacol. Sin., 36, 200 (2001).

W.J. Lu, X.F. Wang, J.Y. Chen, Y. Lv, N. Wu, W.J. Kang and Q.T. Zheng, Acta Pharmacol. Sin., 33, 755 (1998).

U. Himmelreich, M. Masaoud, G. Adam and H. Ripperger, Phytochemistry, 39, 949 (1995); doi:10.1016/0031-9422(95)00044-8.

M. Masaoud, H. Ripperger, U. Himmelreich and G. Adam, Phytochemistry, 38, 751 (1995); doi:10.1016/0031-9422(94)00718-9.

Q.A. Zheng, H.Z. Li, Y.J. Zhang and C.R. Yang, Helv. Chim. Acta, 87, 1167 (2004); doi:10.1002/hlca.200490106.

Z.H. Zhou, J.L. Wang, C.R. Yang, Chinese Tradit. Herbal Drugs, 32, 484 (2001).

F. Liu, R.J. Dai, Y.L. Deng, W.W. Meng and X.Y. Jin, China Pharmacy, 21, 1437 (2010).

D. Gupta and R.K. Gupta, BMC Complement. Altern. Med., 11, 13 (2011); doi:10.1186/1472-6882-11-13.

D. Gupta, B. Bleakley and R.K. Gupta, J. Ethnopharmacol., 115, 361 (2008); doi:10.1016/j.jep.2007.10.018.

N. Xin, Y.J. Li, Y. Li, R.J. Dai, W.W. Meng, Y. Chen, M. Schlappi and Y.-L. Deng, J. Ethnopharmacol., 135, 510 (2011); doi:10.1016/j.jep.2011.03.063.M. Schlappi

T. Yi, H.B. Chen, Z.Z. Zhao, Z.L. Yu and Z.H. Jiang, J. Ethnopharmacol., 133, 796 (2011); doi:10.1016/j.jep.2010.11.008.

J.Y. Deng, Y.M. Li and W.R. Fang, Chin. J. Nat. Med., 4, 373 (2006).

W.R. Fang, Y.N. Li and J.Y. Deng, Chin. J. Clin. Pharmacol. Ther., 10, 1020 (2005).

J.Y. Ren, Q.B. Zhang and W.H. Jiang, ITCM, 23, 31 (2006).

Z.X. Wang, LiShiZhen Med. Mater. Medica Res., 16, 269 (2005).

Y. Li, W. Xiao, J.P. Qin, X. Lin and Z.Z. Wang, Chinese J. Exp. Tradit. Med Formulae, 18, 45 (2012).

Y. Li, W. Xiao, J. Qin, Y. Guo and Q. Zhu, China J. Chinese Mater. Med., 37, 929 (2012).

H. Li and X.L. Sun, Chinese Pharm. Affairs, 24, 696 (2010).

Y.H. Wang, S.Y. Mu, W. Wang and G. Hang, LiShiZhen Med. Mater. Med. Res., 11, 1073 (2000).

Q.L. Song and S.F. Hu, China Pharmacist, 12, 1314 (2009).

D.F. Chen and Q.S. Song, Chin. Tradit. Herbal Drugs, 36, 1545 (2005).

S.L. Sun, H.M. Mi, S.Q. Wu, Q.F. Yao, Y.M. Hu and Y.S. Zheng, Chin. Tradit. Herbal Drugs, 33, 1033 (2002).

W.J. Gong, Y.H. Cao and Y. Wang, Chromatographia, 66, 767 (2007); doi:10.1365/s10337-007-0406-4.

K. Ichikawa, M. Kitaoka, M. Taki, S. Takaishi, Y. Lijima, M. Boriboon and T. Akiyama, Planta Med., 63, 540 (1997); doi:10.1055/s-2006-957759.

G. Nasini, L. Camarda and L. Merlini, Heterocycles, 20, 39 (1983); doi:10.3987/R-1983-01-0039.

Y.Q. Hu, P.F. Tu, R.Y. Li, Z. Wan and R.L. Wang, Chin. Tradit. Herbal Drugs, 32, 104 (2001).

D. Veselá, R. Marek, K. Ubik, K. Lunerová, V. Sklenář and V. Suchý, Phytochemistry, 61, 967 (2002); doi:10.1016/S0031-9422(02)00354-0.

Issue

Vol. 26 No. 13 (2014): Vol 26 Issue 13

Analysis of Phenolic Compounds and Determination of Loureirin B in Dragon's Blood by LC-MS and High Performance Capillary Electrophoresis

Corresponding Author(s) : Hai-Yan Fu

Abstract

Keywords

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