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Vol. 26 No. 12 (2014): Vol 26 Issue 12

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Simultaneous Determination of 13 Nucleosides and Nucleobases in Ganoderma lucidum and Related Species by HPLC-DAD

https://doi.org/10.14233/ajchem.2014.15962
J.L. Peng
1College of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha, P.R. China;State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, P.R. China; These authors contributed to this work equally
Q.X. Peng
Biology Department, Hong Kong Baptist University, Hong Kong;These authors contributed to this work equally
L.M. Lin
College of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha, P.R. China
W.W. Dong
State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, P.R. China
T.S. Liu
College of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha, P.R. China
X.H. Xia
College of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha, P.R. China
D.J. Yang
College of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha, P.R. China ;State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, P.R. China

Corresponding Author(s) : D.J. Yang

dajianyang1@gmail.com

Asian Journal of Chemistry, Vol. 26 No. 12 (2014): Vol 26 Issue 12

Abstract

A simple, sensitive and reliable HPLC-DAD method was performed for the simultaneous determination of 13 nucleosides and nucleobases including cytosine, uracil, cytidine, hypoxanthine, uridine, thymine, 2¢-deoxyuridine, adenine, inosine, guanosine, thymidine, adenosine and 2¢-deoxyadenosine in Ganoderma lucidum and its related species. The analysis was conducted on a phenomenex C18 column (250 mm × 4.6 mm i.d., 5 mm) eluting with a gradient of acetonitrile in water at a flow rate of 0.6 mL/min and the detection wavelength was set as 260 nm. The optimized method attained good linear relation (r2 ³ 0.9996 for 13 analytes), satisfactory precision (RSD < 1.52 % ) and good recovery (from 98.10 to 101.27 %). The validated method was successfully applied to the simultaneous determination of 13 analytes in 42 samples from Ganoderma lucidum and its related species collected from different areas, which could be beneficial to control their quality.

Keywords

Ganoderma lucidum HPLC-DAD Nucleobase Nucleoside Quality control
Peng, J., Peng, Q., Lin, L., Dong, W., Liu, T., Xia, X., & Yang, D. (2014). Simultaneous Determination of 13 Nucleosides and Nucleobases in Ganoderma lucidum and Related Species by HPLC-DAD. Asian Journal of Chemistry, 26(12), 3477–3482. https://doi.org/10.14233/ajchem.2014.15962
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