Copyright (c) 2014 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Chemical Standardization and in vitro Cytotoxic Studies on Nellikai lehyam
Corresponding Author(s) : V. Sudha
Asian Journal of Chemistry,
Vol. 26 No. 12 (2014): Vol 26 Issue 12
Abstract
Plants play a major role in all alternate systems of medicines like Siddha, Ayurveda, Unani and Chinese. In the recent years there has been considerable researches going on traditional siddha medicines focusing towards validation and standardization aspects. In the present work, an immuno-modulatory drug-Nellikai lehyam was subjected to physicochemical standardization and in vitro cytotoxic studies. A preliminary phytochemical screening was carried out to identify the nature of phytoconstituents present. The yield of tannins, flavonoids, phenols, vitamin C, total fatty matter, were estimated as per standard procedures. Percentage of iron was also estimated and was found to be 77.49 %. Finger printing profile of flavonoids in Nellikai lehyam was carried out by HPTLC technique using toluene: ethyl acetate:formic acid (5:4:1 v/v/v) as mobile phase. The bio active constituents in lehyam were also identified through GC-MS and LC-MS analysis. In vitro antioxidant activity was carried out using DPPH and reducing assay. This drug possesses strong antioxidant activity which was exposed by its ability to scavenge the stable free radical DPPH. The cytotoxic activity was tested against cells using MTT assay which revealed strong activity. This is the first report on chemical standardization, antioxidant and anticancer activity of this unique siddha formulation Nellikai lehyam.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
- B.V Subbarayappa, Siddha Medicine: An Overview, Med Cult., pp. 1841-1844 (1997).
- V.A Shanmuga, Sridhar's Science of Longevity and Kalpa Medicines of India, pp. 202-213 (1963).
- Traditional Ethanomedicinal Systems and Alternative Therapies, In: HIV and AIDS: Basic Elements and Priorities, Springer, Ch. 17, pp. 251-271 (2007).
- C. Ngamkitidechakul, K. Jaijoy, P. Hansakul, N. Soonthornchareonnon and S. Sireeratawong, Phytother. Res., 24, 1405 (2010); doi:10.1002/ptr.3127.
- A.K Gupta, N. Tandon and M. Sharma, Quality Standards of Indian Medicinal Plants, Indian Council of Medical Research, New Delhi, Vol. 2, p. 275 (2005).
- Indian Pharmacopoeia, The Controller of Publications, Delhi, Vol. 1, pp. 139, 146, 201 (2010).
- J.B. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, p. 279 (1992).
- A.K Gupta, N. Tandon and M. Sharma, Quality Standards of Indian Medicinal Plants, Indian Council of Medical Research, New Delhi, Vol. 1, p. 209 (2003).
- Ayurvedic Pharmacopoeia of India (Formulations), Part II, vol. 1, p. 243 (2007).
- Indian Pharmacopoeia, The Controller of Publications, Delhi, vol. 1, p. 661 (1996).
- I.C.F.R. Ferreira, P. Baptista, M. Vilas-Boas and L. Barros, Food Chem., 100, 1511 (2007); doi:10.1016/j.foodchem.2005.11.043.
- M. Oyaizu, Japan. J. Nutr., 44, 307 (1986).
- T. Mosmann, J. Immunol. Methods, 65, 55 (1983); doi:10.1016/0022-1759(83)90303-4.
- M. Ken-ichi, T. Murayama, T. Hatano, T. Hatano, T. Yoshida and T. Okuda, Basic Life Sci., 66, 643 (2000).
- P.G. Pietta, J. Nat. Prod., 63, 1035 (2000); doi:10.1021/np9904509.
- N. Saeed, M.R. Khan and M. Shabbir, BMC Complement. Altern. Med., 12, 221 (2012); doi:10.1186/1472-6882-12-221.
- Y.-C. Yoo, B.-H. Shin, J.-H. Hong, J. Lee, H.-Y. Chee, K.-S. Song and K.-B. Lee, Arch. Pharm. Res., 30, 361 (2007); doi:10.1007/BF02977619.
- M. Poljak-Blazi, M. Jaganjac, M. Mustapic, N. Pivac and D. Muck-Seler, Immunobiology, 214, 121 (2009); doi:10.1016/j.imbio.2008.07.005.
- Y.X. Li, Y. Li and Z.J. Qjan, J. Microbiol. Biotechnol., 19, 1319 (2009).
- L. Sui, Y. Dong, Y. Watanabe, F. Yamaquchi, N. Hatano, K. Izumori and M. Tokuda, Anticancer Res., 25, 2639 (2005).
- A.H. Acikelli, S. Gustmann, W. Bardenheuer, J. Klein, U. Dembinski, B. Kohl, K.T. Yip, A. Nazif, R. Stoll, D. Strumberg and D. Díaz-Carballo, Int. J. Clin. Pharmacol. Ther., 51, 51 (2013); doi:10.5414/CPP51051.
References
B.V Subbarayappa, Siddha Medicine: An Overview, Med Cult., pp. 1841-1844 (1997).
V.A Shanmuga, Sridhar's Science of Longevity and Kalpa Medicines of India, pp. 202-213 (1963).
Traditional Ethanomedicinal Systems and Alternative Therapies, In: HIV and AIDS: Basic Elements and Priorities, Springer, Ch. 17, pp. 251-271 (2007).
C. Ngamkitidechakul, K. Jaijoy, P. Hansakul, N. Soonthornchareonnon and S. Sireeratawong, Phytother. Res., 24, 1405 (2010); doi:10.1002/ptr.3127.
A.K Gupta, N. Tandon and M. Sharma, Quality Standards of Indian Medicinal Plants, Indian Council of Medical Research, New Delhi, Vol. 2, p. 275 (2005).
Indian Pharmacopoeia, The Controller of Publications, Delhi, Vol. 1, pp. 139, 146, 201 (2010).
J.B. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, p. 279 (1992).
A.K Gupta, N. Tandon and M. Sharma, Quality Standards of Indian Medicinal Plants, Indian Council of Medical Research, New Delhi, Vol. 1, p. 209 (2003).
Ayurvedic Pharmacopoeia of India (Formulations), Part II, vol. 1, p. 243 (2007).
Indian Pharmacopoeia, The Controller of Publications, Delhi, vol. 1, p. 661 (1996).
I.C.F.R. Ferreira, P. Baptista, M. Vilas-Boas and L. Barros, Food Chem., 100, 1511 (2007); doi:10.1016/j.foodchem.2005.11.043.
M. Oyaizu, Japan. J. Nutr., 44, 307 (1986).
T. Mosmann, J. Immunol. Methods, 65, 55 (1983); doi:10.1016/0022-1759(83)90303-4.
M. Ken-ichi, T. Murayama, T. Hatano, T. Hatano, T. Yoshida and T. Okuda, Basic Life Sci., 66, 643 (2000).
P.G. Pietta, J. Nat. Prod., 63, 1035 (2000); doi:10.1021/np9904509.
N. Saeed, M.R. Khan and M. Shabbir, BMC Complement. Altern. Med., 12, 221 (2012); doi:10.1186/1472-6882-12-221.
Y.-C. Yoo, B.-H. Shin, J.-H. Hong, J. Lee, H.-Y. Chee, K.-S. Song and K.-B. Lee, Arch. Pharm. Res., 30, 361 (2007); doi:10.1007/BF02977619.
M. Poljak-Blazi, M. Jaganjac, M. Mustapic, N. Pivac and D. Muck-Seler, Immunobiology, 214, 121 (2009); doi:10.1016/j.imbio.2008.07.005.
Y.X. Li, Y. Li and Z.J. Qjan, J. Microbiol. Biotechnol., 19, 1319 (2009).
L. Sui, Y. Dong, Y. Watanabe, F. Yamaquchi, N. Hatano, K. Izumori and M. Tokuda, Anticancer Res., 25, 2639 (2005).
A.H. Acikelli, S. Gustmann, W. Bardenheuer, J. Klein, U. Dembinski, B. Kohl, K.T. Yip, A. Nazif, R. Stoll, D. Strumberg and D. Díaz-Carballo, Int. J. Clin. Pharmacol. Ther., 51, 51 (2013); doi:10.5414/CPP51051.