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Vol. 26 No. 12 (2014): Vol 26 Issue 12

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Toxicological Effects of Crude Oil: Integrated Biomarker Responses in the Hepatopancreas of Clam Ruditapes philippinarum

https://doi.org/10.14233/ajchem.2014.16736
Bin Xia
Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, P.R. China
Bijuan Chen
Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, P.R. China
Xuemei Sun
Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, P.R. China
Yi Cui
Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, P.R. China
Jun Zhao
Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, P.R. China
Keming Qu
Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, P.R. China

Corresponding Author(s) : Bin Xia

xiabin@ysfri.ac.cn

Asian Journal of Chemistry, Vol. 26 No. 12 (2014): Vol 26 Issue 12

Abstract

A battery of biomarkers, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferases (GST) and peroxidase (POD) in hepatopancreas, were measured to test the toxicological effects of the water-soluble fraction of crude oil on clam (Ruditapes philippinarum) for period of 1, 2, 4, 8 and 15 days. The results showed that these biomarker responses exhibited a certain dose-effect and time-effect relation. Subsequently, the enzymatic activities were quantified using the integrated biomarker response method. In short-term exposure (1, 2 and 4 days), the superoxide dismutase and catalase (maximum values of integrated biomarker response) were the dominant enzymes. Furthermore, the integrated biomarker response values were well correlated with the concentrations of petroleum in short-term exposure (r2 = 0.521, 0.815 and 0.935 for 1, 2 and 4 days, respectively). These results suggest that the integrated biomarker response approach may be a useful tool to evaluate the sub-lethal effects of petroleum hydrocarbons on marine organism in short-term exposure.

Keywords

Petroleum Integrated biomarker responses Clam Crude oil Ruditapes philippinarum
Xia, B., Chen, B., Sun, X., Cui, Y., Zhao, J., & Qu, K. (2014). Toxicological Effects of Crude Oil: Integrated Biomarker Responses in the Hepatopancreas of Clam Ruditapes philippinarum. Asian Journal of Chemistry, 26(12), 3631–3638. https://doi.org/10.14233/ajchem.2014.16736
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