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Magnetic Dispersion Extraction of Tetracyclines Residues from Milk by Chlortetracycline-Imprinted Magnetic Composite Microspheres
Corresponding Author(s) : Yun-Kai Lv
Asian Journal of Chemistry,
Vol. 26 No. 12 (2014): Vol 26 Issue 12
Abstract
A magnetic dispersion extraction method was developed based on a molecularly imprinted magnetic microsphere for fast separation and analysis of tetracyclines residues in milk samples. The molecularly imprinted magnetic microsphere was prepared by inverse emulsion suspension polymerization and synthesis and extraction conditions were optimized for obtaining excellent affinity and high selectivity. The magnetism, covering amount, clean-up of the magnetic microspheres were characterized by vibrating sample magnetometer, thermogravimetric analysis and HPLC. The molecularly imprinted magnetic microspheres were applied to separate tetracycline antibiotics from milk by magnetic dispersion extraction with a good sample clean-up effect. The average recoveries of four tetracycline antibiotics were obtained in the range of 76.4-95.84 % with precision 2.72-6.81 %. The limits of detection and quantitation of the proposed method were in the range of 5.71-11.18 and 19.02-37.28 μg/kg, respectively. The results indicated that magnetic dispersion extraction of the molecularly imprinted magnetic microspheres was powerful for the food sample pretreatment with high selectivity and simplified procedure.
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- R.J. Ansell and K. Mosbach, Analyst, 123, 1611 (1998); doi:10.1039/a801903g.
- Y. Zhang, R.J. Liu, Y.L. Hu and G.K. Li, Anal. Chem., 81, 967 (2009); doi:10.1021/ac8018262.
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- X.W. Kan, Z.R. Geng, Y. Zhao, Z.L. Wang and J.J. Zhu, Nanotechnology, 20, 165601 (2009); doi:10.1088/0957-4484/20/16/165601.
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- C. Gonzato, M. Courty, P. Pasetto and K. Haupt, Adv. Funct. Mater., 21, 3947 (2011); doi:10.1002/adfm.201100466.
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- Standardization Administration of China, GB/T 22990-2008, Determi-nation of Oxytetracycline, Tetracycline, Chlortetracycline, Doxycycline Residues in Milk and Milk Powder-HPLC-UV Method.
- B. Sellergren, M. Lepistoe and K. Mosbach, J. Am. Chem. Soc., 110, 5853 (1988); doi:10.1021/ja00225a041.
- Y.K. Lv, L.M. Wang, L. Yang, C.X. Zhao and H.W. Sun, J. Chromatogr. A, 1227, 48 (2012); doi:10.1016/j.chroma.2011.12.108.
References
R.J. Ansell and K. Mosbach, Analyst, 123, 1611 (1998); doi:10.1039/a801903g.
Y. Zhang, R.J. Liu, Y.L. Hu and G.K. Li, Anal. Chem., 81, 967 (2009); doi:10.1021/ac8018262.
S.L. Lu, G.X. Cheng, H.G. Zhang and X.S. Pang, J. Appl. Polym. Sci., 99, 3241 (2006); doi:10.1002/app.22997.
C.J. Tan, H.G. Chua, K.H. Ker and Y.W. Tong, Anal. Chem., 80, 683 (2008); doi:10.1021/ac701824u.
X.W. Kan, Z.R. Geng, Y. Zhao, Z.L. Wang and J.J. Zhu, Nanotechnology, 20, 165601 (2009); doi:10.1088/0957-4484/20/16/165601.
L. Li, X.W. He, L.X. Chen and Y.K. Zhang, Chem. Asian J., 4, 286 (2009); doi:10.1002/asia.200800300.
C. Gonzato, M. Courty, P. Pasetto and K. Haupt, Adv. Funct. Mater., 21, 3947 (2011); doi:10.1002/adfm.201100466.
Q.Q. Gai, F. Qu, Z.J. Liu, R.J. Dai and Y.K. Zhang, J. Chromatogr. A, 1217, 5035 (2010); doi:10.1016/j.chroma.2010.06.001.
Y.L. Liu, Y.Y. Huang, J.Z. Liu, W.Z. Wang, G.Q. Liu and R. Zhao, J. Chromatogr. A, 1246, 15 (2012); doi:10.1016/j.chroma.2012.01.045.
Standardization Administration of China, GB/T 22990-2008, Determi-nation of Oxytetracycline, Tetracycline, Chlortetracycline, Doxycycline Residues in Milk and Milk Powder-HPLC-UV Method.
B. Sellergren, M. Lepistoe and K. Mosbach, J. Am. Chem. Soc., 110, 5853 (1988); doi:10.1021/ja00225a041.
Y.K. Lv, L.M. Wang, L. Yang, C.X. Zhao and H.W. Sun, J. Chromatogr. A, 1227, 48 (2012); doi:10.1016/j.chroma.2011.12.108.