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Vol. 26 No. 11 (2014): Vol 26 Issue 11

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Enhancing the Production of Human-Like Collagen II by Adding L-Methionine During High-Cell-Density Fermentation of Recombinant Escherichia coli

https://doi.org/10.14233/ajchem.2014.17517
Lei Guo
Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, P.R. China ;Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, P.R. China
Yan'e Luo
Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, P.R. China ;Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, P.R. China
Daidi Fan
Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, P.R. China ;Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, P.R. China

Corresponding Author(s) : Yan'e Luo

luoyane@nwu.edu.cn

Asian Journal of Chemistry, Vol. 26 No. 11 (2014): Vol 26 Issue 11

Abstract

Recombinant Escherichia coli BL21 was used to produce human-like collagen II (HLC) in fed-batch cultivation. The effects of feeding strategies of L-methionine on Yp/x (yield coefficient, HLC/DCW, g g-1), OD600 and acetate were investigated. In order to gain the highest Yp/x, L-methionine feeding strategy was optimized. The optimum operation was that 1.2 g/L of L-methionine should be added at the start of thermal induction. When 1.2 g/L of L-methionine was added at the start of thermal induction, Yp/x and cell OD600 could reach 0.2 g g-1 and 137 and they were raised by 25 and 9.6 %, respectively, while the concentration of by-products acetate was reduced to the safety level for cell growth and HLC synthesis.

Keywords

Fed-batch culture Human-like collagen II L-Methionine Recombinant Escherichia coli.
Guo, L., Luo, Y., & Fan, D. (2014). Enhancing the Production of Human-Like Collagen II by Adding L-Methionine During High-Cell-Density Fermentation of Recombinant Escherichia coli. Asian Journal of Chemistry, 26(11), 3315–3319. https://doi.org/10.14233/ajchem.2014.17517
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