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Microbial Community Changes of Crude Oil Polluted Soil During Combined Remediation
Corresponding Author(s) : Hong Qi Wang
Asian Journal of Chemistry,
Vol. 26 No. 11 (2014): Vol 26 Issue 11
Abstract
The changes of microbial community structure are important indicators which indicate the effect of remediation of the oily soil. In this study, winter wheat and a high-efficiency degradation strain Pseudomonas sp. DG17 isolated from oil-contaminated soil were joined up to degrade petroleum hydrocarbon. The bacteria were in two forms: immobilized bacteria and bacteria inoculum. After 70 days, the combination of winter wheat and immobilized bacteria DG17 could degrade up to 18.09 % petroleum hydrocarbon, showing great potential in repairing oiled soil. The diversity of rhizosphere microorganisms which included the microorganism function diversity and the genetic diversity was analyzed by some emerging molecular biology methods. It was found that with the degradation of the petroleum hydrocarbon, the carbon source utilization types and dominant bacteria varied a lot in all treatments. During the late period of the experiment, functional bacteria which could degrade petroleum hydrocarbon better appeared.
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- M. Narayanan, L.C. Davis, J.C. Tracy, L.E. Erickson and R.M. Green, J. Hazard. Mater., 41, 229 (1995); doi:10.1016/0304-3894(94)00113-U.
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- J.L. Garland, Soil Biol. Biochem., 28, 213 (1996); doi:10.1016/0038-0717(95)00112-3.
- J.L. Garland and A.L. Mills, Appl. Environ. Microbiol., 57, 2351 (1991).
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- I.M. Head, J.R. Saunders and R.W. Pickup, Microb. Ecol., 35, 1 (1998); doi:10.1007/s002489900056.
- C. Valenzuela-Encinas, I. Neria-González, R.J. Alcántara-Hernández, I. Estrada-Alvarado, F.J. Zavala-Díaz de la Serna, L. Dendooven and R. Marsch, Extremophiles, 13, 609 (2009); doi:10.1007/s00792-009-0244-4.
- K. Tamura, J. Dudley, M. Nei and S. Kumar, Mol. Biol. Evol., 24, 1596 (2007); doi:10.1093/molbev/msm092.
- Y. Kasai, H. Kishira, K. Syutsubo and S. Harayama, Environ. Microbiol., 3, 246 (2001); doi:10.1046/j.1462-2920.2001.00185.x.
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References
M. Narayanan, L.C. Davis, J.C. Tracy, L.E. Erickson and R.M. Green, J. Hazard. Mater., 41, 229 (1995); doi:10.1016/0304-3894(94)00113-U.
K.A. Reilley, M.K. Banks and A.P. Schwab, J. Environ. Qual., 25, 212 (1996); doi:10.2134/jeq1996.00472425002500020002x.
Å. Frostegård and E. Bååth, Biol. Fertil. Soils, 22, 59 (1996); doi:10.1007/BF00384433.
J.L. Garland, Soil Biol. Biochem., 28, 213 (1996); doi:10.1016/0038-0717(95)00112-3.
J.L. Garland and A.L. Mills, Appl. Environ. Microbiol., 57, 2351 (1991).
J.K. Fredrickson, D.L. Balkwill, J.M. Zachara, S.-M. W. Li, F.J. Brockman and M.A. Simmons, Appl. Environ. Microbiol., 57, 402 (1991).
J.B. Guckert, G.J. Carr, T.D. Johnson, B.G. Hamm, D.H. Davidson and Y. Kumagai, J. Microbiol. Methods, 27, 183 (1996); doi:10.1016/S0167-7012(96)00948-7.
J.T. Hollibaugh, Microb. Ecol., 28, 117 (1994); doi:10.1007/BF00166800.
I.M. Head, J.R. Saunders and R.W. Pickup, Microb. Ecol., 35, 1 (1998); doi:10.1007/s002489900056.
C. Valenzuela-Encinas, I. Neria-González, R.J. Alcántara-Hernández, I. Estrada-Alvarado, F.J. Zavala-Díaz de la Serna, L. Dendooven and R. Marsch, Extremophiles, 13, 609 (2009); doi:10.1007/s00792-009-0244-4.
K. Tamura, J. Dudley, M. Nei and S. Kumar, Mol. Biol. Evol., 24, 1596 (2007); doi:10.1093/molbev/msm092.
Y. Kasai, H. Kishira, K. Syutsubo and S. Harayama, Environ. Microbiol., 3, 246 (2001); doi:10.1046/j.1462-2920.2001.00185.x.
W.F. Röling, M.G. Milner, D.M. Jones, K. Lee, F. Daniel, R.J.P. Swannell and I.M. Head, Appl. Environ. Microbiol., 68, 5537 (2002); doi:10.1128/AEM.68.11.5537-5548.2002.
R.M. Abed, N.M. Safi, J. Köster, D. de Beer, Y. El-Nahhal, J. Rullkotter and F. Garcia-Pichel, Appl. Environ. Microbiol., 68, 1674 (2002); doi:10.1128/AEM.68.4.1674-1683.2002.