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Vol. 26 No. 11 (2014): Vol 26 Issue 11

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Mechanisms of Generation of Biogenic Methane Influenced by Types of Strain and Disodium EDTA

https://doi.org/10.14233/ajchem.2014.17509
Daping Xia
School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, P.R. China ;State Key Laboratory Cultivation Base for Gas Geology and Gas Control, Jiaozuo 454000, P.R. China
Zhen Wang
School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, P.R. China
Xianbo Su
School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, P.R. China ;State Key Laboratory Cultivation Base for Gas Geology and Gas Control, Jiaozuo 454000, P.R. China
Junqiang Ma
School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, P.R. China

Corresponding Author(s) : Daping Xia

569689819@qq.com

Asian Journal of Chemistry, Vol. 26 No. 11 (2014): Vol 26 Issue 11

Abstract

In adoption of plate isolation, anaerobic culture and PCR amplification techniques, a methanogens-culture experiment was conducted for investigating the mechanisms of generation of biogenic methane, during which coals of Shaqu colliery were used as substrates and mine water collected from Zhongtai and Shaqu collieries, respectively. The white-rot fungi preserved in laboratory were utilized as strain sources. Methane production was fully examined with the addition of disodium EDTA under various concentration gradients. The results show that there are methanogens present in mine water of Zhongtai, while the growth of methanogens in mine water of Shaqu is possibly discouraged due to the tested existence of denitrifying bacteria and overhigh pH. Hence, there is no methane being produced from coals of Shaqu as compared to the occurrence of gaseous production from that of Zhongtai under the same conditions. Meanwhile, when disodium EDTA is added at 1 g/L can the CH4 production reach a peak of 10 mL/g after a period of increase and the pH value can also see a notable rise after reaction.

Keywords

Methanogens Biogenic methane Disodium EDTA Gaseous production
Xia, D., Wang, Z., Su, X., & Ma, J. (2014). Mechanisms of Generation of Biogenic Methane Influenced by Types of Strain and Disodium EDTA. Asian Journal of Chemistry, 26(11), 3270–3274. https://doi.org/10.14233/ajchem.2014.17509
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