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Determination of Vanillin in Milk Powder by Rapid Column High Performance Liquid Chromatography
Corresponding Author(s) : Chang-Li Zhao
Asian Journal of Chemistry,
Vol. 26 No. 10 (2014): Vol 26 Issue 10
Abstract
A rapid column high performance liquid chromatography method for the determination of vanillin in milk powder was studied. The vanillin was extracted from the sample with 40 % methanol by the ultrasonic extraction and then was purified by solid phase extraction with C18 cartridge. Vanillin was separated on a ZORBAX stable bound (4.6 × 50 mm, 1.8 mm) rapid chromatographic column with water-methonal (65:35) as mobile phase at a flow-rate of 2 mL/min and monitored with the photodiode array detector at 309 nm. The recoveries of the vinillin are 98-103 % and the relative standard deviations are 0.46-0.58 %. This method was applied to the determination of vanillin in milk powder with good results.
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References
M. Baig, G. Kapse and S. Raju, Asian J. Chem., 13, 185 (2001).
Y. Huang, Z. Lu and G. Lu, Chinese J. Health Lab., 12, 566 (2002).
L. Nagpurkar, A. Chaudhari and J. Ekhe, Asian J. Chem., 14, 1387 (2002).
D. Reddy, B. Gurupadayya, Y. Manohara and V. Bhaskar, Asian J. Chem., 19, 2797 (2007).
F. Rind, U. Mughal, A. Memon, F. Almani and M. Laghari, Asian J. Chem., 21, 2849 (2009).
A.J. Aktas, Asian J. Chem., 22, 3719 (2010).
M. Chen, J. Wang, Y. Zhang and K. Meng, Asian J. Chem., 24, 1029 (2012).