Extractive Spectrophotometric Method for Determination of Anti-Viral Drug Ribavirin Using Eriochrome Black-T

Nawal A. Alarfaj; Reda A. Ammar; Maha F. El-Tohamy

doi:10.14233/ajchem.2014.16316

Issue

Vol. 26 No. 10 (2014): Vol 26 Issue 10

Issue Published : May 10, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Extractive Spectrophotometric Method for Determination of Anti-Viral Drug Ribavirin Using Eriochrome Black-T

https://doi.org/10.14233/ajchem.2014.16316

Nawal A. Alarfaj

Deparartment of Chemistry, College of Science, King Saud University P.O. Box 22452, Riyadh 11495, Saudi Arabia

Reda A. Ammar

Deparartment of Chemistry, College of Science, King Saud University P.O. Box 22452, Riyadh 11495, Saudi Arabia; Deparartment of Chemistry, College of Science, Al-Azhar University, Cairo, Egypt

Maha F. El-Tohamy

Deparartment of Chemistry, College of Science, King Saud University P.O. Box 22452, Riyadh 11495, Saudi Arabia

Corresponding Author(s) : Nawal A. Alarfaj

nalarfaj@hotmail.com

Asian Journal of Chemistry, Vol. 26 No. 10 (2014): Vol 26 Issue 10
Article Published : May 10, 2014

Abstract

A new extractive spectrophotometric method for determination of anti-viral drug ribavirin was developed and validated. The proposed method was based on the formation of red colored complex with Eriochrome black-T in acidic medium. The absorbance of extractive organic layer with chloroform was measured at 576 nm. Beer's law was obeyed revealed that the linear concentration range was 2-120 μg/mL (r = 0.999) with lower detection and quantification limits 0.87 and 1.76 μg/mL, respectively. The proposed method was validated according to (ICH) guidelines and successfully applied for the determination of the drug in bulk powder and pharmaceutical formulations.

Keywords

Ribavirin Eriochrome black-T Spectrophotometric method Ion-pair complexation

A. Alarfaj, N., A. Ammar, R., & F. El-Tohamy, M. (2014). Extractive Spectrophotometric Method for Determination of Anti-Viral Drug Ribavirin Using Eriochrome Black-T. Asian Journal of Chemistry, 26(10), 2992–2996. https://doi.org/10.14233/ajchem.2014.16316

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References

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I.A. Darwish, A.S. Khedr, H.F. Askal and R.M. Mahmoud, IL Farmaco, 60, 555 (2005); doi:10.1016/j.farmac.2005.04.003.
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J. Sulkowska, A. Budych and R. Stroscik, Chem. Anal., 30, 387 (1985).
D.B. Patil and D.M. Chafle, Asian J. Chem., 19, 3253 (2007).
B. Rajitha, S. Prashanthi, K.R. Ramsubha and G.R. Tulja, Int. J. Pharm. Tech. Res., 3, 114 (2011).
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J.C. Miller and J.N. Miller, Statistics for Analytical Chemistry, Ellis Horwood-Prentice Hall, Chichester, edn 3 (1993).

References

L.J. Scott and C.M. Perry, Drugs, 62, 507 (2002); doi:10.2165/00003495-200262030-00009.

M. Homma, A.L. Jayewardene, J. Gambertoglio and F. Aweeka, Antimicrob. Agents Chemother., 43, 2716 (1999).

A. Loregian, M.C. Scarpa, S. Pagni, S.G. Parisi and G. Palù, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 856, 358 (2007); doi:10.1016/j.jchromb.2007.05.039.

L.-T. Yeh, M. Nguyen, S. Dadgostari, W. Bu and C.-C. Lin, J. Pharm. Biomed. Anal., 43, 1057 (2007); doi:10.1016/j.jpba.2006.09.001.

W. Li, S. Luo, S. Li, L. Athill, A. Wu, T. Ray, W. Zhou, J. Ke, H.T. Smith and F.L.S. Tse, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 846, 57 (2007); doi:10.1016/j.jchromb.2006.08.014.

M. Meléndez, O. Rosario, B. Zayas and J.F. Rodríguez, J. Pharm. Biomed. Anal., 49, 1233 (2009); doi:10.1016/j.jpba.2009.02.006.

E. Zironi, T. Gazzotti, B. Lugoboni, A. Barbarossa, A. Scagliarini and G. Pagliuca, J. Pharm. Biomed. Anal., 54, 889 (2011); doi:10.1016/j.jpba.2010.11.021.

I.A. Darwish, H.F. Askal, A.S. Khedr and R.M. Mahmoud, J. Chromatogr. Sci., 46, 4 (2008); doi:10.1093/chromsci/46.1.4.

M.C. Breadmore, R. Theurillat and W. Thormann, Electrophoresis, 25, 1615 (2004); doi:10.1002/elps.200305819.

E.M.K. Sharaf, M.A. El-Brashy, S.Z. Awad and E.M. Rania, J. AOAC Int., 89, 631 (2006).

I.A. Darwish, K.S. Alaa, A.F. Hassan and M.M. Ramadan, J. AOAC Int., 89, 341 (2006).

N.A. Alarfaj and M.F. El-Tohamy, J. Indian Chem. Soc., 90, 1 (2013).

N.A. Alarfaj and M.F. El-Tohamy, Luminescence, 28, 190 (2013); doi:10.1002/bio.2361.

I.A. Darwish, A.S. Khedr, H.F. Askal and R.M. Mahmoud, IL Farmaco, 60, 555 (2005); doi:10.1016/j.farmac.2005.04.003.

D.P. Quan, D.P. Tuyen, T.D. Lam, P.T.N. Tram, N.H. Binh and P.H. Viet, Colloid. Surf. B, 88, 764 (2011); doi:10.1016/j.colsurfb.2011.08.012.

A.E. Harvey, J.M. Komarmy and G.M. Wyatt, Anal. Chem., 25, 498 (1953); doi:10.1021/ac60075a031.

J. Sulkowska, A. Budych and R. Stroscik, Chem. Anal., 30, 387 (1985).

D.B. Patil and D.M. Chafle, Asian J. Chem., 19, 3253 (2007).

B. Rajitha, S. Prashanthi, K.R. Ramsubha and G.R. Tulja, Int. J. Pharm. Tech. Res., 3, 114 (2011).

G.R. Tulja, S.D. Gowri, P. Kadgapathi, R. Suthakaran and B. Satyanarayana, Int. J. Pharm. Tech. Res., 2, 1787 (2010).

A.S. Amin, A.D. Hassan and A.A. Ibrahim, Arab. J. Chem., l, 209 (2008).

D.B. Patil and D.M. Chafle, Asian J. Chem., 19, 3253 (2007).

N. Rahman, N. Ahmad Khan and S.N. Hejaz Azmi, IL Farmaco, 59, 47 (2004); doi:10.1016/j.farmac.2003.10.001.

J. Rose, Advanced Physico-Chemial Experiments, Pitman, London, p. 54 (1964).

ICH Technical Requirements for Registration of Pharmaceuticals for Human Use, Complementary Guidelines on Methodology, Washington, DC, vol. 13 (1996).

J.C. Miller and J.N. Miller, Statistics for Analytical Chemistry, Ellis Horwood-Prentice Hall, Chichester, edn 3 (1993).

Issue

Vol. 26 No. 10 (2014): Vol 26 Issue 10

Extractive Spectrophotometric Method for Determination of Anti-Viral Drug Ribavirin Using Eriochrome Black-T

Corresponding Author(s) : Nawal A. Alarfaj

Abstract

Keywords

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