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Vol. 26 No. 9 (2014): Vol 26 Issue 9

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Determination of Total Cholesterol in Serum by Gas Chromatography-Mass Spectrometry

https://doi.org/10.14233/ajchem.2014.15780
Kaoqi Lian
The School of Public Health, Hebei Medical University, Shijiazhuang 050017, P.R. China
Pingping Zhang
Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, P.R. China
Wei Wang
The School of Public Health, Hebei Medical University, Shijiazhuang 050017, P.R. China
Tingting Dai
The School of Public Health, Hebei Medical University, Shijiazhuang 050017, P.R. China
Lei Li
Department of Hygienic Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing 211166, P.R. China

Corresponding Author(s) : Lei Li

drleili@hotmail.com

Asian Journal of Chemistry, Vol. 26 No. 9 (2014): Vol 26 Issue 9

Abstract

In this study, a reliable protocol for determination of total cholesterol in serum using gas chromatography-mass spectrometry was established. The total free cholesterol was extracted from the serum with chloroform and derivatized with bis(trimethylsilyl)trifluoroacetamide, analyzed without the saponification step commonly used. Linear calibration curves of cholesterol were obtained from the concentration 0.1 to 15 mmol L-1. The limit of detection was 0.04 mmol L-1. Finally, the proposed method was to explore the influence of high cholesterol diet on total cholesterol in serum of rats.

Keywords

Cholesterol Gas chromatography-mass spectrometry Serum High cholesterol diet
Lian, K., Zhang, P., Wang, W., Dai, T., & Li, L. (2014). Determination of Total Cholesterol in Serum by Gas Chromatography-Mass Spectrometry. Asian Journal of Chemistry, 26(9), 2646–2648. https://doi.org/10.14233/ajchem.2014.15780
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