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Vol. 34 No. 3 (2022): Vol 34 Issue 3, 2022

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Synthesis of Gold Nanoparticles using Endophytic streptomyces and its Bactericidal Activities against Enteric Bacterial Pathogens

https://doi.org/10.14233/ajchem.2022.23587
S. Farook Basha
P.G. & Research Department of Chemistry, Jamal Mohamed College (Autonomous) (Affiliated to Bharathidasan University), Tiruchirappalli-620020, Ind
P. Sivalingam
P.G. & Research Department of of Microbiology, Jamal Mohamed College (Autonomous) (Affiliated to Bharathidasan University), Tiruchirappalli-620020, India
Manickam Muthuselvam
Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirapppalli-620024, India

Corresponding Author(s) : Manickam Muthuselvam

muthuphd@gmail.com

Asian Journal of Chemistry, Vol. 34 No. 3 (2022): Vol 34 Issue 3, 2022

Abstract

The present study aimed to report the rapid and one-step synthesis of gold nanoparticles (AuNPs) using the cell-free supernatant (CFS) of an endophytic Streptomyces sp. BDUSMP S05 isolated from Solanum trilobatum. The synthesized AuNPs were characterized by using UV-visible spectroscopy (UV-vis), Fourier-transform infrared spectroscopy (FTIR), energy dispersive X-ray (EDAX) spectroscopy and X-ray diffraction (XRD). The UV-visible spectroscopy (UV-vis) absorption of colloidal AuNPs displayed a surface plasma resonance (SPR) centred at 533 nm. FESEM micrograph analysis showed that synthesized particles were predominantly spherical with a 20-30 nm size range. Dynamic light scattering (DLS) analysis showed that the average particle size varied between 2-28 nm. Zeta potential analysis indicated the negative value of -35.2 mV characteristic for stable AuNPs. The synthesized AuNPs displayed significant antimicrobial activity against Gram-negative bacteria, including Eescherichia coli, Salmonella typhi and Vibrio cholerae compared to CFS alone. The results revealed that endophytic Streptomyces sp. potential to be used for the synthesis of antibacterial gold nanobased materials.

Keywords

Endophytic Streptomyces Extracellular biosynthesis Gold nanoparticles Antibacterial activity
Farook Basha, S., Sivalingam, P., & Muthuselvam, M. (2022). Synthesis of Gold Nanoparticles using Endophytic streptomyces and its Bactericidal Activities against Enteric Bacterial Pathogens. Asian Journal of Chemistry, 34(3), 607–613. https://doi.org/10.14233/ajchem.2022.23587
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