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Effect of Extraction Techniques on Total Phenolics, Flavonoids Contents and Antioxidant Activity of Various Solvent Fractions of Bark of Arjun (Terminalia arjuna)
Corresponding Author(s) : V.K. Madan
Asian Journal of Chemistry,
Vol. 29 No. 3 (2017): Vol 29 Issue 3
Abstract
The objective of the present work was to investigate the effect of extraction techniques viz. cold (mechanical shaking) and hot (Soxhlet) on total phenolics and flavonoids as well as on the antioxidative potential of methanolic extracts of Terminalia arjuna bark and its fractions in solvents of different polarities. Results revealed that extract yield (12.623 g/100 g), total phenolics content (45.377 mg GAE/g), flavonoids content (15.071 mg CE/g) and DPPH free radical scavenging activity (IC50: 134.8 μg/mL) were found to be higher in methanolic extract obtained by hot extraction technique in comparison to cold extraction technique. In various solvent fractions of methanolic extracts of bark of Arjun obtained by both extraction techniques, total phenolics and flavonoids contents were found to be highest in residual aqueous fraction followed by butanol, ethyl acetate, dichloromethane, chloroform and hexane fractions. The methanolic extracts obtained by both extraction techniques, ethyl acetate fraction exhibited the highest antioxidant activity followed by butanol, residual aqueous, chloroform, dichloromethane and hexane fractions.
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- O.K. Chun, D.O. Kim and C.Y. Lee, J. Agric. Food Chem., 51, 8067 (2003).
- L. Yu, S. Haley, J. Perret, M. Harris, J. Wilson and M. Qian, J. Agric. Food Chem., 50, 1619 (2002).
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- P. Li, L. Huo, W. Su, R. Lu, C. Deng, L. Liu, Y. Deng, N. Guo, C. Lu and C. He, J. Serb. Chem. Soc., 76, 709 (2011).
- K.E. Heim, A.R. Tagliaferro and D.J. Bobilya, J. Nutr. Biochem., 13, 572 (2002).
- B.J. Xu and S.K. Chang, J. Food Sci., 72, S159 (2007).
- U.P. Singh, D.P. Singh, S. Maurya, R. Maheshwari, M. Singh, R.S. Dubey and R.B. Singh, J. Herb. Pharmacother., 4, 27 (2004).
- S.R. Anjaneyulu and V.R. Prasad, Indian J. Chem., 21, 530 (1982).
- A. Ghani, Medicinal Plants of Bangladesh, pp. 1-2, 55-57, 402, 500 edn 2 (2003).
- S. Mohammad, A. Sadika, I.H. Md, A.H. Md and A.B. Mohiuddin, J. Med. Plants Res., 6, 5286 (2012).
- V.L. Singleton and J.A. Rossi, J. Enol. Vitic., 16, 144 (1965).
- D. Marinova, F. Ribarova and M. Atanassova, J. Univ. Chem. Technol. Metallur., 40, 255 (2005).
- T. Hatano, H. Kagawa, T. Yasuhara and T. Okuda, Chem. Pharm. Bull. (Tokyo), 36, 2090 (1988).
- R. Scherer and H.T. Godoy, Rev. Brasil. Plantas Med., 16, 41 (2014).
- A.A.R. Sayed, Evid. Based Complement. Alternat. Med., 2012, 1 (2012).
- S. Akhter, M.I. Hossain, M.A. Haque, M. Shahriar and M.A. Bhuyian, Eur. J. Scientific Res., 86, 543 (2012).
- I. Singh, V.K. Madan, S.S. Jangra and S. Singh, Asian J. Chem., 28, 801 (2016).
- N.T.R. Meda, M.J. Bangou, S. Bakasso, J. Millogo-Rasolodimby and O.G. Nacoulma, J. Appl. Pharm. Sci., 3, 36 (2013).
- A. Maimoona, I. Naeem, Z. Saddique, N. Ali, G. Ahmed and I. Shah, J. Med. Plants Res., 5, 2724 (2011).
- R.A. Sai, C.J. Godwin and V. Thankamani, Res. J. Pharm. Technol., 6, 996 (2013).
- S.S. Jangra, V.K. Madan and S. Singh, J. Indian Chem. Soc., 92, 1149 (2015).
- M.A. Hossain, K.A.S. AL-Raqmi, Z.H. AL-Mijizy, A.M. Weli and Q. Al-Riyami, Asian Pac. J. Trop. Biomed., 3, 705 (2013
References
O.K. Chun, D.O. Kim and C.Y. Lee, J. Agric. Food Chem., 51, 8067 (2003).
L. Yu, S. Haley, J. Perret, M. Harris, J. Wilson and M. Qian, J. Agric. Food Chem., 50, 1619 (2002).
E. Vági, E. Rapavi, M. Hadolin, K. Vásárhelyiné Perédi, A. Balázs, A. Blázovics and B. Simándi, J. Agric. Food Chem., 53, 17 (2005).
P. Li, L. Huo, W. Su, R. Lu, C. Deng, L. Liu, Y. Deng, N. Guo, C. Lu and C. He, J. Serb. Chem. Soc., 76, 709 (2011).
K.E. Heim, A.R. Tagliaferro and D.J. Bobilya, J. Nutr. Biochem., 13, 572 (2002).
B.J. Xu and S.K. Chang, J. Food Sci., 72, S159 (2007).
U.P. Singh, D.P. Singh, S. Maurya, R. Maheshwari, M. Singh, R.S. Dubey and R.B. Singh, J. Herb. Pharmacother., 4, 27 (2004).
S.R. Anjaneyulu and V.R. Prasad, Indian J. Chem., 21, 530 (1982).
A. Ghani, Medicinal Plants of Bangladesh, pp. 1-2, 55-57, 402, 500 edn 2 (2003).
S. Mohammad, A. Sadika, I.H. Md, A.H. Md and A.B. Mohiuddin, J. Med. Plants Res., 6, 5286 (2012).
V.L. Singleton and J.A. Rossi, J. Enol. Vitic., 16, 144 (1965).
D. Marinova, F. Ribarova and M. Atanassova, J. Univ. Chem. Technol. Metallur., 40, 255 (2005).
T. Hatano, H. Kagawa, T. Yasuhara and T. Okuda, Chem. Pharm. Bull. (Tokyo), 36, 2090 (1988).
R. Scherer and H.T. Godoy, Rev. Brasil. Plantas Med., 16, 41 (2014).
A.A.R. Sayed, Evid. Based Complement. Alternat. Med., 2012, 1 (2012).
S. Akhter, M.I. Hossain, M.A. Haque, M. Shahriar and M.A. Bhuyian, Eur. J. Scientific Res., 86, 543 (2012).
I. Singh, V.K. Madan, S.S. Jangra and S. Singh, Asian J. Chem., 28, 801 (2016).
N.T.R. Meda, M.J. Bangou, S. Bakasso, J. Millogo-Rasolodimby and O.G. Nacoulma, J. Appl. Pharm. Sci., 3, 36 (2013).
A. Maimoona, I. Naeem, Z. Saddique, N. Ali, G. Ahmed and I. Shah, J. Med. Plants Res., 5, 2724 (2011).
R.A. Sai, C.J. Godwin and V. Thankamani, Res. J. Pharm. Technol., 6, 996 (2013).
S.S. Jangra, V.K. Madan and S. Singh, J. Indian Chem. Soc., 92, 1149 (2015).
M.A. Hossain, K.A.S. AL-Raqmi, Z.H. AL-Mijizy, A.M. Weli and Q. Al-Riyami, Asian Pac. J. Trop. Biomed., 3, 705 (2013