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Vol. 29 No. 3 (2017): Vol 29 Issue 3

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Variation in Total Phenolics, Flavonoids and Antioxidant Activity Among Various Solvent Fractions of Bark of Babul (Acacia nilotica) Using Different Extraction Techniques

https://doi.org/10.14233/ajchem.2017.20291
Pravesh
Medicinal, Aromatic and Potential Crops Section, Old IATTE Building, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125 004, India
V.K. Madan
Medicinal, Aromatic and Potential Crops Section, Old IATTE Building, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125 004, India
Sushila Singh
Department of Chemistry and Biochemistry, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125 004, India

Corresponding Author(s) : V.K. Madan

vikku60@gmail.com

Asian Journal of Chemistry, Vol. 29 No. 3 (2017): Vol 29 Issue 3

Abstract

In present study, extraction techniques such as cold (mechanical shaking) and hot (Soxhlet) extraction were compared to evaluate their efficiency towards extraction of total phenolics and flavonoids. The bark of babul was extracted with methanol and further fractionated into various solvent fractions (viz. hexane, dichloromethane, chloroform, ethyl acetate and butanol). The methanol extract and its fractions were used for the estimation of total phenolics & flavonoids contents and for the evaluation of antioxidant activity by 2,2’-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity method. The methanol extract obtained by hot extraction technique possessed higher total phenolics and flavonoids contents i.e. 49.380 and 18.895 mg CE/g, respectively and also exhibited higher activity with IC50 value 67.7 μg/mL. Among various solvent fractions using both extraction techniques, ethyl acetate fraction contained highest amount of total phenolics (9.369 and 9.508 mg GAE/g) and flavonoids (3.229 and 3.786 mg CE/g) followed by butanol, residual aqueous, dichloromethane, chloroform and hexane fractions. Similarly, ethyl acetate fraction exhibited highest antioxidant activity (IC50 24.9 and 35.2 μg/mL) followed by butanol, residual aqueous/dichloromethane, chloroform and hexane fractions.

Keywords

Acacia nilotica Extraction techniques Solvent fractions Total phenolics Flavonoids Antioxidant activity
Pravesh, ., Madan, V., & Singh, S. (2016). Variation in Total Phenolics, Flavonoids and Antioxidant Activity Among Various Solvent Fractions of Bark of Babul (Acacia nilotica) Using Different Extraction Techniques. Asian Journal of Chemistry, 29(3), 641–646. https://doi.org/10.14233/ajchem.2017.20291
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