Study on the Effect of Dual Solvent Proportions on Composition of Rosa x damascena Concrete Oil Obtained using Soxhlet Extraction Method

Abstract

Concrete oil was extracted from Rosa x damascena using different percentage ratios of solvents (petroleum ether and ethanol) by the Soxhlet extraction method. The extraction was carried out using petroleum ether and ethanol in five different percentage ratios of (v/v) (100:0, 75:25, 50:50, 25:75, 0:100) (petroleum ether:ethanol). The rotary vacuum evaporator was used to separate concrete oil and the solvents. The extracted concrete oil was analyzed using gas chromatography-mass spectrometry (GC-MS) technique. The obtained results show that many new compounds were identified, at two different solvents and its ratios. Phenylethyl alcohol in the percentages of (61.71%), (10.07%) and (25.92%) was obtained as a major compound with the solvent percentages of (100:0), (50:50) and (75:25) (PE:E), respectively. Hexacosane (37.2%) was identified as a major compound when pure ethanol is used as a solvent. The highest number of components were identified (totally 93 components) when an equal percentage (50:50) of petroleum ether and ethanol were mixed. The usual monoterpenes components, e.g. geraniol, nerol, citronellol and linalool, were not found in the present extraction study. This study concludes that the compositions of concrete oil were mainly influenced by the type of solvents and its ratios used for the extraction.